RAN基因沉默对辐射诱发基因组不稳定肝细胞HL-7702凋亡及细胞周期的影响

辐射防护 ›› 2014, Vol. 34 ›› Issue (6): 361-365.

RAN基因沉默对辐射诱发基因组不稳定肝细胞HL-7702凋亡及细胞周期的影响

党旭红, 左雅慧, 原雅艺, 刘红艳, 刘建功, 张睿凤

中国辐射防护研究院, 太原 030006

收稿日期:2013-06-07 出版日期:2014-11-20 发布日期:2025-01-07
通讯作者: 左雅慧。E-mail:yahuiz@163.com
作者简介:党旭红(1983—),男,2006年毕业于山西大学生物科学专业,2009年毕业于山西大学生物化学与分子生物学专业,获硕士学位,助理研究员。E-mail:dangxuhong005@163.com

Effects of RAN Gene Silencing by siRNA on Apoptosis and Cell Cycle in Radiation Induced Genomic Instable Liver Cells

Dang Xuhong, ZuoYahui, Yuan Yayi, Liu Hongyan, Liu Jiangong, Zhang Ruifeng

China Institute for Radiation Protection, Taiyuan 030006

Received:2013-06-07 Online:2014-11-20 Published:2025-01-07

摘要/Abstract

摘要： 为研究RAN基因沉默对基因组不稳定肝细胞凋亡及细胞周期的影响,利用慢病毒介导的RNA干扰(RNAi)技术沉默基因组不稳定肝细胞中RAN基因的表达,通过流式细胞术检测RAN基因沉默后基因组不稳定肝细胞的凋亡率及细胞周期。实时荧光定量PCR检测表明,RAN siRNA能有效沉默RAN基因mRNA的转录(p＜0.01);流式细胞术检测表明,RAN基因沉默诱发基因组不稳定肝细胞G₀/G₁期细胞增加(p＜0.05),发生G₁期阻滞,同时,细胞凋亡率较阴性对照组明显降低(p＜0.01)。说明RAN基因可能通过参与周期调控及促进细胞凋亡进而维持肝细胞基因组的稳定性。

关键词: RAN基因, 基因组不稳定, RNA干扰(RNAi), 细胞周期, 细胞凋亡

Abstract: RNAi was used to inhibit RAN gene’s transcription and expression level of RAN. The apoptosis was detected by Annex in Ⅴ-APC flow cytometry (FCM) and the cell cycle was determined by FCM with single PI staining. Results of real time fluorescence quantitative PCR tests demonstrated that RAN gene can be inhibited efficiently by RNAi. The apoptosis percentage in RNAi group was lower than that in the negative control, the number of cells in G₁ phase were higher than in the negative control. These results indicated that RAN gene may maintain the genomic stability through regulating the cell apoptosis and cell cycles.

Key words: RAN gene, genomic instability, RNA interference, cell cycle, cell apoptosis

中图分类号:

党旭红, 左雅慧, 原雅艺, 刘红艳, 刘建功, 张睿凤. RAN基因沉默对辐射诱发基因组不稳定肝细胞HL-7702凋亡及细胞周期的影响[J]. 辐射防护, 2014, 34(6): 361-365.

Dang Xuhong, ZuoYahui, Yuan Yayi, Liu Hongyan, Liu Jiangong, Zhang Ruifeng. Effects of RAN Gene Silencing by siRNA on Apoptosis and Cell Cycle in Radiation Induced Genomic Instable Liver Cells[J]. RADIATION PROTECTION, 2014, 34(6): 361-365.

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