γ射线照射人肝细胞对胰岛素样生长因子相关基因表达的影响

辐射防护 ›› 2014, Vol. 34 ›› Issue (2): 102-108.

γ射线照射人肝细胞对胰岛素样生长因子相关基因表达的影响

李建国, 袁慧, 尹晶晶, 秦秀军, 张伟, 闻建华

中国辐射防护研究院,太原 030006

收稿日期:2012-09-26 出版日期:2014-03-20 发布日期:2025-01-03
作者简介:李建国(1976—),男,2004年毕业于山西农业大学分子遗传专业,副研究员。E-mail:ljg2547@163.com
基金资助:
“十二五基础科研”资助项目(项目编号:1201110202)。

Study of γ-ray Irradiation with Expressive Influence of IGF Related Genes in Human Hepatocytes

Li Jianguo, Yuan Hui, Yin Jingjing, Qin Xiujun, Zhang Wei, Wen Jianhua

China Institute for Radiation Protection, Taiyuan 030006

Received:2012-09-26 Online:2014-03-20 Published:2025-01-03

摘要/Abstract

摘要： 利用实时荧光定量PCR技术对人肝细胞株受不同剂量(0.1、0.2、0.5、1.0、2.0、4.0 Gy)⁶⁰Co γ射线照射后不同时间点(6、12、24 h)的胰岛素样生长因子相关基因进行剂量和时间效应关系研究。结果表明,胰岛素样生长因子1受体(IGF1R)在不同剂量及照后不同时间点PCR扩增结果均表现为低表达趋势;胰岛素样生长因子结合蛋白1(IGFBP1)在不同剂量照后6 h点PCR扩增结果均表现为高表达趋势,在照后12 h点均表现为低表达趋势,而在照后24 h点除个别剂量点表现为高表达趋势(0.1 Gy和0. 2 Gy点),其余剂量点均表现为低表达趋势;胰岛素样生长因子2结合蛋白3(IGF2BP3)除个别剂量点和时间点PCR扩增结果表现为低表达趋势(0.2 Gy照后6、12、24 h及0.5 Gy照后6 h),其余各剂量点和时间点均表现为高表达趋势;胰岛素样生长因子结合蛋白4(IGFBP4)在不同剂量照后6 h点PCR扩增结果均表现为低表达趋势,而在照后12、24 h点则表现为高表达趋势。

关键词: γ射线, 人肝细胞株, 胰岛素样生长因子, 实时荧光定量PCR

Abstract: The study analyzed the effect-relationship of γ-ray irradiation with different doses (0.1, 0.2, 0.5, 1, 2 and 4 Gy) and different times(6, 12 and 24 h) of IGF related genes in human hepatocytes by real-time fluorescent quantitative PCR. The results indicated that insulin-like growth factor-1 receptor (IGF1R) showed low expression with different dosage and duration; insulin-like growth factor binding protein-1 (IGFBP1) showed high expression after radiation 6 h, 24 h showed low expression, 0.1 and 0.2 Gy showed high expression and 0.5, 1, 2 and 4 Gy low expression after radiation 12 h; IGF2BP3 showed low expression with 0.2 Gy and 6 h of 0.5 Gy, and high expression with other dosage and duration; IGFBP4 showed low expression with 6 h, and high expression with 12 and 24 h.

Key words: gamma-ray, human hepatocytes, insulin-like growth factor (IGF), real-time fluorescent quantitative PCR

中图分类号:

Q343

李建国, 袁慧, 尹晶晶, 秦秀军, 张伟, 闻建华. γ射线照射人肝细胞对胰岛素样生长因子相关基因表达的影响[J]. 辐射防护, 2014, 34(2): 102-108.

Li Jianguo, Yuan Hui, Yin Jingjing, Qin Xiujun, Zhang Wei, Wen Jianhua. Study of γ-ray Irradiation with Expressive Influence of IGF Related Genes in Human Hepatocytes[J]. RADIATION PROTECTION, 2014, 34(2): 102-108.

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