电离辐射对断乳期小鼠脾脏发育的影响研究

辐射防护 ›› 2021, Vol. 41 ›› Issue (5): 467-472.

电离辐射对断乳期小鼠脾脏发育的影响研究

郑宇栋¹, 刘元朵¹, 王长征², 黄江荣¹, 刘莲¹

1.长江大学医学部基础医学院,湖北荆州 434023;
2.长江大学第二临床医学院,湖北荆州 434023

收稿日期:2020-10-21 出版日期:2021-09-20 发布日期:2021-11-26
通讯作者: 刘莲。E-mail:liulian@yangtzeu.edu.cn
作者简介:郑宇栋(1994—),男,2018年毕业于长江大学中西医结合临床专业,2019年至今长江大学基础医学专业硕士生在读。E-mail:zheng.yudong@foxmail.com
基金资助:
湖北省自然科学基金“海马NR2B DNA去甲基化介导孕期尼古丁暴露致子代抑郁症易感”(2018CFB667);2016年长江大学青年科技创新团队基金“神经发育毒性研究”(2016cqt04);湖北省卫生健康委青年人才项目 “低剂量射线暴露所致小鼠海马发育影响及其机制研究” (WJ2021Q015)。

Effect of ionizing radiation on spleen development of mice during weaning age

ZHENG Yudong¹, LIU Yuanduo¹, WANG Changzheng², HUANG Jiangrong¹, LIU Lian¹

1. School of Basic Medicine, Health Science Center, Yangtze University, Hubei Jingzhou 434023;
2. The Second Clinical Medical College, Yangtze University, Hubei Jingzhou 434023

Received:2020-10-21 Online:2021-09-20 Published:2021-11-26

摘要/Abstract

摘要： 为探讨电离辐射对断乳期小鼠脾脏发育及其机制的影响,将14只21日龄C57小鼠随机分为对照组和照射组,每组7只,照射组接受5 Gy的急性X射线全身照射,于照射后7天取血并处死小鼠取材。称取小鼠体重及脾重,生化法检测小鼠外周血象的变化,免疫组织化学和Western blot法检测小鼠脾脏中磷酸化H₂AX蛋白的表达。结果表明, 与对照组相比,照射组小鼠的体重及脾重均明显降低(p < 0.01),外周血中白细胞、血小板、红细胞数量以及血红蛋白的含量均减少(p< 0.05, p < 0.01),脾脏中磷酸化H₂AX蛋白的表达降低(p < 0.05, p < 0.01)。实验结果证明断乳期小鼠受到X射线照射可导致小鼠脾脏发育抑制及其功能异常,这可能与辐射抑制H₂AX磷酸化表达从而降低DNA修复能力相关。

关键词: X射线, 脾脏, H₂AX, DNA损伤

Abstract: This experiment investigates the effects and mechanism of ionizing radiation on spleen development of mice during weaning age. Totally fourteen C57 mice with 21-days-old were randomly divided into two groups (control group and irradiation group, each with seven mice). The mice in the irradiation group have received the whole body X-ray radiation with the dose of 5 Gy. The samples were taken from mouse of each group at seven days after radiation respectively. The body weight and spleens were weighed and calculated spleen indicators. Biochemical method was utilized to detect the changes of peripheral blood of mice. Immunohistochemistry and western blotting were used to detect the protein expression of phospho-H₂AX in spleen. Compared to the control group,the body weights and the spleen weights of mice in the irradiation group were significantly reduced (p<0.01), indicating that radiation can significantly reduce the content of white blood cells, platelets, red blood cells and hemoglobin, as well as the expression of phospho-H₂AX (p<0.05, p<0.01). X-ray exposure could resulted abnormal functions of the spleen in weaning age mice, which may be related to the decreased expression of p-H₂AX, thus reducing the ability of DNA repair.

Key words: X-ray, spleen, H₂AX, DNA damage

中图分类号:

Q691

郑宇栋, 刘元朵, 王长征, 黄江荣, 刘莲. 电离辐射对断乳期小鼠脾脏发育的影响研究[J]. 辐射防护, 2021, 41(5): 467-472.

ZHENG Yudong, LIU Yuanduo, WANG Changzheng, HUANG Jiangrong, LIU Lian. Effect of ionizing radiation on spleen development of mice during weaning age[J]. RADIATION PROTECTION, 2021, 41(5): 467-472.

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