γ射线照射人肝细胞对BMP信号通路相关基因表达的影响

辐射防护 ›› 2016, Vol. 36 ›› Issue (4): 211-217.

γ射线照射人肝细胞对BMP信号通路相关基因表达的影响

尹晶晶, 李建国, 秦秀军, 李钢, 黄立群, 袁慧, 闻建华

中国辐射防护研究院药物毒理与放射损伤药物山西省重点实验室,太原 030006

收稿日期:2015-05-22 出版日期:2016-07-20 发布日期:2021-11-15
通讯作者: 闻建华。E-mail: jianhua570621@163.com
作者简介:尹晶晶(1982—),女,2010年毕业于山西大学细胞生物学专业,助理研究员。E-mail: yjingjing1982@163.com
基金资助:
山西省青年科技研究基金项目(项目编号:2013021033-4)。

Effects of gamma ray on the expression of BMP pathway related genes in human hepatocytes

Yin Jingjing, Li Jianguo, Qin Xiujun, Li Gang, Huang Liqun, Yuan Hui, Wen Jianhua

China Institute for Radiation Protection,Shanxi Key Laboratory of Drug Toxicology and Drug for Radiation Injury, Taiyuan 030006

Received:2015-05-22 Online:2016-07-20 Published:2021-11-15

摘要/Abstract

摘要： 利用实时荧光定量PCR技术对人肝细胞株受不同剂量(0.1、0.2、0.5、1.0、2.0、4.0 Gy)⁶⁰Co γ射线照后不同时间点(6、12、24 h)的骨形成蛋白(BMP)信号通路相关基因表达的剂量和时间效应关系进行研究。结果显示,不同剂量照后不同时间点,BMP信号通路相关基因骨形成蛋白2(BMP2)、骨形成蛋白7(BMP7)、骨形成蛋白受体1A(BMPR1A)、骨形成蛋白受体1B(BMPR1B)、骨形成蛋白受体2(BMPR2)的溶解曲线均为单峰,均为特异性扩增产物。在不同照射剂量点BMP2的表达水平与照后时间呈正相关;BMP7在0.1、0.2、0.5 Gy的表达水平与照后时间呈负相关,在照后12和24 h的表达水平与照射剂量呈正相关;BMPR1A在0.1、0.2、1.0 Gy的表达水平与照后时间呈正相关;BMPR1B在0.2、0.5、1.0 Gy的表达水平与照后时间呈正相关,在4.0 Gy的表达水平与照后时间呈负相关;BMPR2在照后12 h的表达水平与照射剂量呈负相关。

关键词: 人肝细胞株, γ射线, BMP信号通路, 基因表达

Abstract: To study the irradiation effects at different doses (0.1, 0.2, 0.5,1.0,2.0,4.0 Gy) and different time (6,12,24 h) on the expression of BMP pathway related genes in human hepatocytes by real-time quantitative PCR. Results Solution curves of β-actin, BMP2, BMP7, BMPR1A, BMPR1B, and BMPR2 with different doses and time were single peak, showed the specific amplification. The expression levels of BMP2 were positively correlated with time; the expression levels of BMP7 were negatively correlated with time at doses of 0.1, 0.2, and 0.5 Gy, and positively correlated with dose at 12 and 24 h after irradiation; the expression levels of BMPR1A were positively correlated with time at doses of 0.1, 0.2, and 1.0 Gy; the expression levels of BMPR1B were positively correlated with time at doses of 0.2, 0.5, and 1.0 Gy, and negatively correlated with time at dose of 4.0 Gy; the expression levels of BMPR2 were negatively correlated with dose at 12 h after irradiation.

Key words: human hepatocytes, gamma ray, BMP pathway, gene expression

中图分类号:

Q344

尹晶晶, 李建国, 秦秀军, 李钢, 黄立群, 袁慧, 闻建华. γ射线照射人肝细胞对BMP信号通路相关基因表达的影响[J]. 辐射防护, 2016, 36(4): 211-217.

Yin Jingjing, Li Jianguo, Qin Xiujun, Li Gang, Huang Liqun, Yuan Hui, Wen Jianhua. Effects of gamma ray on the expression of BMP pathway related genes in human hepatocytes[J]. RADIATION PROTECTION, 2016, 36(4): 211-217.

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