枸杞多糖对受X射线照射小鼠外周血象及骨髓单个核细胞的影响

辐射防护 ›› 2016, Vol. 36 ›› Issue (4): 218-223.

枸杞多糖对受X射线照射小鼠外周血象及骨髓单个核细胞的影响

李文波¹, 庞华¹, 周静¹, 吴宏², 姜蓉²

1.重庆医科大学附属第一医院核医学科,重庆,400016;
2.重庆医科大学基础医学院组织与胚胎学教研室,重庆,400016

收稿日期:2015-11-19 出版日期:2016-07-20 发布日期:2021-11-15
通讯作者: 庞华。E-mail:phua1973@163.com
作者简介:李文波(1987—),男,2012年毕业于湘南学院临床医学系,现为重庆医科大学影像与核医学硕士研究生。E-mail:bobosatm@163.com
基金资助:
重庆市自然科学基金(cstc2013jcyjA10072)。

Effects of lyceum barbarum polysaccharide (LBP) on peripheral blood and bone marrow mononuclear cells of X ray irradiated mice

Li Wenbo¹, Pang Hua¹, Zhou Jing¹, Wu Hong², Jiang Rong²

1. Department of Nuclear Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016;
2. Department of Histology and Embryology, College of Basic Medicine, Chongqing Medical University, Chongqing 400016

Received:2015-11-19 Online:2016-07-20 Published:2021-11-15

摘要/Abstract

摘要： 利用直线加速器全身均匀一次性照射昆明小鼠,建立放射损伤模型,经腹腔分别注射生理盐水(NS)、枸杞多糖(LBP)50、100和200 mg/kg,连续注射观察14天,计数小鼠外周血WBC、RBC、PLT及骨髓单个核细胞(BMNCs),检测BMNCs的细胞周期、凋亡率及BMNCs表面黏附分子CD49d和CD44的表达水平。结果表明,照射后小鼠表现为饮水、进食减少,行动缓慢、嗜睡,毛发凌乱,光泽性差,说明放射损伤模型建立成功;在相同时间点下,NS组与正常对照组相比,外周血WBC、RBC、PLT及BMNCs细胞计数明显减少(p<0.05)、BMNCs的细胞增殖能力明显减弱(p<0.05)、S期细胞比例显著减少(p<0.05)、G₀/G₁期细胞比例显著增加、BMNCs的细胞凋亡率明显增多(p<0.05)、黏附分子CD49d和CD44表达明显减少(p<0.05);而各LBP组与NS组相比,外周血各项指标、BMNCs细胞计数明显增多(p<0.05)、BMNCs细胞的增殖能力明显增强(p<0.05)、S期细胞比例显著增多(p<0.05)、G₀ /G₁期细胞比例显著减少、细胞凋亡率明显减少 (p<0.05)、黏附分子CD49d、CD44表达明显增加(p<0.05)。以上结果说明,LBP可能通过加速BMNCs细胞周期由G₀ /G₁期向S期转换、降低BMNCs细胞凋亡率,提高放射损伤小鼠BMNCs细胞的增殖能力,进而上调细胞表面黏附分子CD49d和CD44的表达,来促进辐射损伤小鼠造血功能的恢复。

关键词: 枸杞多糖, 放射损伤, 骨髓单核细胞, 细胞周期, 细胞凋亡

Abstract: Lycium barbarum polysaccharide (LBP) has anti radiation damage effect. Animal models of radiation injury were established by exposing Kunming mice to the linear accelerator X ray once. The irradiated mice were given intra-peritoneal injection with NS, LBP 50 mg/kg, LBP 100 mg/kg and LBP 200 mg/kg for 14 days respectively. The counts of WBC, RBC, PLT in peripheral blood and BMNCs were recorded BMNCs cell cycle cell apoptotic ratio and BMNCs surface adhesion molecule CD49d and CD44 were detected to illuminate the molecular mechanism of LBP in sheltering radiation injured haematogenesis. Experimental results show that: After irradiation, the mice were observed drank and ate less than before, slow of movement, lethargy, messy hair and poor of glossiness, demonstrating the success of radiation damage model establishment; At the same time, NS group compared with the normal control group, Peripheral blood WBC, RBC, PLT and BMNCs count decreased significantly (p<0.05), the cell proliferation of BMNCs was significantly decreased (p<0.05), the proportion of cells in S phase significantly decreased (p< 0.05), G₀/G₁phase cell percentage was significantly increased, the apoptotic rate of BMNCs cells increased significantly (p< 0.05), cell adhesion molecule CD49d and CD44 expression was significantly reduced (p< 0.05); At the same time, compared with the NS groups, the results of LBP groups showed that the numbers of peripheral blood cells and BMNCs significant increased (p<0.05), the cell proliferation of BMNCs was significantly increased (p< 0.05), the proportion of cells in S phase increased significantly (p<0.05), G₀/G₁phase cell percentage was significantly decreased, the apoptotic rate was significantly lower (p< 0.05), adhesion molecule CD49d and CD44 expression was significantly higher (p< 0.05). In summary for BMNCs, LBP may enhance cell proliferation, accelerate cell cycle transition from G₀/G₁ phase to S phase, reduce apoptotic ratio and up-regulate the expression of cell surface adhesion molecules CD49d and CD44, and finally promote the recovery of hematopoietic function in mice after radiation injury.

Key words: lycium barbarum polysaccharide (LBP), radiation injury, bone marrow mononuclear (BMNC), cell cycle, apoptosis

中图分类号:

Q691

李文波, 庞华, 周静, 吴宏, 姜蓉. 枸杞多糖对受X射线照射小鼠外周血象及骨髓单个核细胞的影响[J]. 辐射防护, 2016, 36(4): 218-223.

Li Wenbo, Pang Hua, Zhou Jing, Wu Hong, Jiang Rong. Effects of lyceum barbarum polysaccharide (LBP) on peripheral blood and bone marrow mononuclear cells of X ray irradiated mice[J]. RADIATION PROTECTION, 2016, 36(4): 218-223.

参考文献

[1] 胡妮. 枸杞多糖单用及联合CXCL10抗肝癌作用免疫机制研究[J]. 广州中医药大学学报, 2015, 12(2):212-216.
[2] 刘文达,衣服新,惠青山. 枸杞多糖联合卡铂对人胶质瘤U251细胞生长抑制和凋亡作用[J]. 解放军医学院学报, 2013, 34(2): 179-180, 188.
[3] Luo Q, Li J, Cui X,et al.The effect of lycium barbarum polysaccharides on the male rats reproductive system and spermatogenic cell apoptosis exposed to low-dose ionizing irradiation[J]. Ethnopharmacol, 2014,154(1):249-258.
[4] Yi R, Liu X, Dong Q. A study of lycium barbarum polysaccharides (LBP) extraction technology and its anti-aging effect[J]. African Journal of Traditional, Complementary and Alternative Medicines, 2013,10(4):193-198.
[5] 楚建杰,鹿成韬,王艳华.多糖对电离辐射防护与修复作用的研究进展[J]. 中国药师, 2015,18(6):1 001-1 004.
[6] Gong H, Shen P, Jin L, et al. Therapeutic effects of lycium barbarum polysaccharide (LBP) on irradiation or chemotherapy-induced myelosuppres-sive mice[J]. Cancer Biotherapy & Radiopharmaceuticals, 2005, 20(2): 155-162.
[7] 中华人民共和国科学技术部.关于善待实验动物的指导性意见[EB/OL]. ( 2006-09-13)[2011-09-20].http://www. busy.edu.cn/dwsyzx/zc/gjzcfg/30367.shtml.
[8] Chen BJ, Deoliveira D, Spasojevic I, et al. Growth hormone mitigates against lethal irradiation and enhances hematologic and immune recovery in mice and nonhuman primates[J]. Plos One, 2010, 5(6): 11 056-11 062.
[9] Chen M, Chen W, Lai C, et al. Predictive factors of radiation-induced skin toxicity in breast cancer patients[J]. Bmc Cancer, 2010, 10(1): 508-514.
[10] 关雪晶,吴宏,何晓莉,等. 当归多糖对辐射损伤小鼠骨髓基质细胞的影响[J]. 第三军医大学学报, 2013, 35(8): 779-783.
[11] Jaklevic B, Uyetake L, Lemstra W, et al. Contribution of growth and cell cycle checkpoints to radiation survival in drosophila[J]. Genetics, 2006, 174(4): 1 963-1 972.
[12] Wong SH, Lowes KN, Bertoncello I, et al. Evaluation of Sca-1 and C-kit as selective markers for muscle remodelling by nonhemopoietic bone marrow cells[J]. Stem Cells, 2007, 25(6): 1 364-1 374.
[13] Ulia Christine Gutjahr1,Richard Greil,Tanja Nicole Hartmann.The role of CD44 in the pathophysiology of chronic lymphocytic leukemia [J].Frontiers in Immunology,2015,6(177):1-7.
[14] Anna S-K,Janusz S,Katarzyna H,et al.Polymorphism of CD44 influences the efficacy of CD34 cells mobilization in patients with hematological malignancies[J]. Biol Blood Marrow Transplant, 2014, 20: 986-991.
[15] Sara Massena, Gustaf Christoffersson, Evelina Vagesjö, et al.Identification and characterization of VEGF-A–responsive neutrophils expressing CD49d, VEGFR1, and CXCR4 in mice and humans[J]. Blood, 2015, 126(17):2 016-2 026.
[16] Vermeulen M, Le pesteur F, Gagnerault M, et al. Role of adhesion molecules in the homing and mobilization of murine hematopoietic stem and progenitor cells[J]. Blood, 1998, 92(3): 894-900.