AuNPs@MPA-PEG纳米粒子对肿瘤细胞辐射敏感性影响

辐射防护 ›› 2016, Vol. 36 ›› Issue (3): 154-159.

AuNPs@MPA-PEG纳米粒子对肿瘤细胞辐射敏感性影响

马洪鸽, 刘瑞芳, 洪承皎, 张保国

苏州大学医学部放射医学与防护学院江苏省高校放射医学协同创新中心,江苏苏州 215123

收稿日期:2015-05-22 出版日期:2016-05-20 发布日期:2021-11-12
通讯作者: 张保国。E-mail:bgzhang@suda.edu.cn
作者简介:马洪鸽(1989—),女,2012年毕业于山西医科大学汾阳学院医学影像学专业,现为苏州大学放射医学专业在读硕士研究生。E-mail:hgma2012@126.com
基金资助:
江苏省高校优势学科建设项目(PAPD)资助。

Effects of AuNPs@MPA-PEG nanoparticles on radiosensitivity of Hela cancer cells

Ma Hongge, Liu Ruifang, Hong Chengjiao, Zhang Baoguo

School of Radiation Medicine and Protection, Medical College of Soochow University, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Jiangsu Suzhou 215123

Received:2015-05-22 Online:2016-05-20 Published:2021-11-12

摘要/Abstract

摘要： 为研究3-巯基丙酸(MPA)-聚乙二醇(PEG)修饰的金纳米粒子(AuNPs@MPA-PEG)对人宫颈癌Hela细胞辐射敏感性的影响,以柠檬酸钠为稳定剂,硼氢化钠为还原剂,还原氯金酸制备5.4 nm的金纳米粒子(AuNPs),并分别用MPA-PEG、PEG修饰金纳米粒子,制备AuNPs@MPA-PEG、AuNPs@PEG。通过电感耦合等离子体发射光谱仪检测Hela细胞及细胞核对AuNPs、AuNPs@MPA-PEG、AuNPs@PEG的吸收量;克隆形成法测定AuNPs、AuNPs@MPA-PEG、AuNPs@PEG对X射线照射后Hela细胞存活率的影响。结果表明,Hela细胞和细胞核对AuNPs、AuNPs@MPA-PEG、AuNPs@PEG的吸收量具有浓度依赖性。MPA-PEG、PEG修饰减少细胞对金纳米粒子的吸收,但是MPA-PEG对金纳米粒子进行修饰,却增加细胞核对金纳米粒子的吸收。对于160 kVp X射线,AuNPs、AuNPs@MPA-PEG、AuNPs@PEG均对Hela细胞有辐射增敏作用,但增敏效果不明显。MPA-PEG、PEG修饰没有增加金纳米粒子的辐射增敏作用。

关键词: 氯金酸, 金纳米粒子, Hela细胞, 辐射敏感性

Abstract: To investigate the effects of AuNPs@MPA-PEG on radiosensitivity of human uterine cervix cancer cell line (Hela), aqueous solutions of AuNPs about 5.4 nm were synthesized by sodium borohydride reduction of tetrachloroauric (III) acid solution in the presence of sodium citrate. Gold nanoparticles (AuNPs) were modified with MPA-PEG or PEG. The intracellular uptake of AuNPs, AuNPs@MPA-PEG and AuNPs@PEG was detected using inductively-coupled plasma ectrometer. Colon forming assay was carried out to study the cells survival rate of Hela cells after exposure to AuNPs,AuNPs@MPA-PEG and AuNPs@PEG combined with radiation. The number of AuNPs, AuNPs@MPA-PEG and AuNPs@PEG in cells and nuclei had dependence on concentration. Gold nanoparticles modified by MPA-PEG or PEG couldn’t promote cells to absorb themselves. But gold nanoparticles modified by MPA-PEG were helpful to the nuclei uptake of gold nanoparticles. At 160 kV X ray energy, AuNPs,AuNPs@MPA-PEG and AuNPs@PEG all could increase the radiation sensitivity of Hela cells. Modification by MPA-PEG or PEG couldn’t increase significantly the radiation sensitivity of Hela cells by comparison with AuNPs.

Key words: gold chloride, gold nanoparticles, Hela cells, radiosensitivity

中图分类号:

R811.5

马洪鸽, 刘瑞芳, 洪承皎, 张保国. AuNPs@MPA-PEG纳米粒子对肿瘤细胞辐射敏感性影响[J]. 辐射防护, 2016, 36(3): 154-159.

Ma Hongge, Liu Ruifang, Hong Chengjiao, Zhang Baoguo. Effects of AuNPs@MPA-PEG nanoparticles on radiosensitivity of Hela cancer cells[J]. RADIATION PROTECTION, 2016, 36(3): 154-159.

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