60Co γ射线照射人肝癌细胞诱导PIF1基因mRNA变化

辐射防护 ›› 2016, Vol. 36 ›› Issue (3): 149-153.

⁶⁰Co γ射线照射人肝癌细胞诱导PIF1基因mRNA变化

王彬^1,3, 张莹^2,3, 赵德根⁴, 李忠秋^2,3, 杨洋^2,3, 李超^1,3, 朱茂祥³, 顾永清^2,3

1.石河子大学生命科学学院, 新疆石河子 832003;
2.石河子大学医学院, 新疆石河子 832003;
3.军事医学科学院放射与辐射医学研究所, 北京 100850;
4.蚌埠市第三人民医院,安徽蚌埠 233000

收稿日期:2016-01-11 出版日期:2016-05-20 发布日期:2021-11-12
通讯作者: 顾永清。E-mail:yqgu96@163.com
作者简介:王彬(1989—),男,2013年6月毕业于塔里木大学生物技术专业,现为石河子大学生物化学与分子生物学专业硕士研究生。E-mail:390361566@qq.com
基金资助:
国家自然科学基金资助项目(No.30960093;No. 31160183; No. 31270894; No.31470827)。

Changes of PIF1 gene expression induced by ⁶⁰Co γ ray in HepG2

Wang Bin^1,3, Zhang Ying^2,3, Zhao Degen⁴, Li Zhongqiu^2,3, Yang Yang^2,3, Li Chao^1,3, Zhu Maoxiang³, Gu Yongqing³

1. College of Life Sciences Shihezi University,Xinjiang Shihezi 832003;
2. College of Medicine Shihezi University, Xinjiang Shihezi 832003;
3. Institute of Radiology and Radiation Medicine,the Chinese Academy of Military Medicine, Beijing 100850;
4. Bengbu Third People's Hospital,Anhui Bengbu 233000

Received:2016-01-11 Online:2016-05-20 Published:2021-11-12

摘要/Abstract

摘要： 分别用2 Gy和4 Gy ⁶⁰Co γ射线照射人肝细胞HepG2,利用real-time PCR方法检测PIF1基因mRNA表达量的变化,以探讨PIF1基因是否参与电离辐射致DNA损伤应答。结果表明,不同剂量的γ射线照射HepG2细胞后,PIF1 mRNA表现出一致的变化规律:即照后先轻微降低随后一直高表达,高表达至少持续到照射后12 h。提示电离辐射能够诱导PIF1表达,表达水平与剂量、时间相关。

关键词: PIF1解螺旋酶, 电离辐射, DNA损伤修复, 实时荧光定量PCR

Abstract: HepG2 cells were irradiated by 2 Gy and 4 Gy ⁶⁰Co γ ray, the mRNA was extracted and real-time PCR was used to detect the changes of PIF1 mRNA, to explore whether PIF1 involved in DNA damaged respond after irradiation. The results showed that PIF1 gene expression level in HepG2 decreased slightly at first and then increased after different doses of ⁶⁰Co γ ray, and the high expression continued about 12 h. PIF1 gene expression can be induced by ionizing radiation indicating its roles in DNA damage response, and the expression level is related to radiation doses and time.

Key words: PIF1 helicase, ionizing radiation, DNA damage and repair, real-time PCR

中图分类号:

Q344

王彬, 张莹, 赵德根, 李忠秋, 杨洋, 李超, 朱茂祥, 顾永清. ⁶⁰Co γ射线照射人肝癌细胞诱导PIF1基因mRNA变化[J]. 辐射防护, 2016, 36(3): 149-153.

Wang Bin, Zhang Ying, Zhao Degen, Li Zhongqiu, Yang Yang, Li Chao, Zhu Maoxiang, Gu Yongqing. Changes of PIF1 gene expression induced by ⁶⁰Co γ ray in HepG2[J]. RADIATION PROTECTION, 2016, 36(3): 149-153.

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⁶⁰Co γ射线照射人肝癌细胞诱导PIF1基因mRNA变化

Changes of PIF1 gene expression induced by ⁶⁰Co γ ray in HepG2

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