低剂量UVB辐射对HaCaT细胞凋亡与增殖及Bad信号通路的影响

辐射防护 ›› 2014, Vol. 34 ›› Issue (3): 168-171.

低剂量UVB辐射对HaCaT细胞凋亡与增殖及Bad信号通路的影响

薛浙南¹, 黄欣², 曹迪¹, 陈瑾¹, 黄琨¹, 陈爱军¹

1.重庆医科大学附属第一医院皮肤科,重庆 400016;
2.重庆医科大学中医药学院方剂教研室,重庆 400016

收稿日期:2013-12-31 出版日期:2014-05-20 发布日期:2025-01-24
通讯作者: 陈爱军。E-mail:cajhx@aliyun.com
作者简介:薛浙南(1986—),男,现为重庆医科大学皮肤病与性病学专业硕士研究生。E-mail: hrzphd@gmail.com
基金资助:
重庆市科技计划项目(cstc2011jjA0115)。

Apoptosis in HaCaT and Bad Pathways

Xue Zhenan¹, Huang Xin², Cao Di¹, Chen Jin¹, Huang Kun¹, Chen Aijun¹

1. Department of Dermatology, The first affiliated hospital of Chongqing Medical School, Chongqing 400016;
2. Institue of traditional Chinese medicine prescription teaching and research center, The first affiliated hospital of Chongqing Medical School, Chongqing 400016

Received:2013-12-31 Online:2014-05-20 Published:2025-01-24

摘要/Abstract

摘要： 为研究低强度紫外线UVB(Ultraviolet B,280～315 nm)对人永生化细胞(HaCaT细胞)凋亡和增殖的影响及其与Bad通路的潜在机制,首先通过预实验从3、5和7 mJ/cm²强度的UVB中挑选出5 mJ/cm²强度的UVB分别照射HaCaT细胞18、36和72 h,收集细胞进行细胞凋亡和增殖检测;提取照射后HaCaT细胞的总蛋白进行免疫印迹实验检测AKT、Bad和Bad-p153的表达。结果表明,5 mJ/cm²强度的UVB照射HaCaT细胞18、36和72 h,对HaCaT细胞的凋亡没有影响,但是照射72 h可以促进HaCaT细胞的增殖;免疫印迹实验证实UVB照射后Bad的表达量维持正常,而AKT和Bad-p153表达升高。说明长时间5 mJ/cm²强度的UVB照射可能通过Bad信号通路促进细胞增殖,这可能是皮肤肿瘤发生发展的潜在危险因素。

关键词: 细胞凋亡, 细胞增殖, 紫外线

Abstract: To study the mechanism of low-doses of ultraviolet B (UVB, 280—315 nm) on human immortalized cells (HaCaT cells) apoptosis, proliferation and Bad pathway, chose the most suitable 5 mJ/cm² intensity of UVB from 3,5 and 7 mJ/cm²to irradiate the HaCaT cells for 18, 36 and 72 hours, then, collected the cells for the detection of apoptosis and proliferation; The total protein in HaCaT cells was extracted from the irradiated cell for western blot to detect the expression of AKT, Bad and the Bad-p136. The results showed that the apoptotic effects of 5 mJ/cm² intensity irradiation on HaCaT cells have no distinguish difference among different time point. But the proliferation rate after 72 hours irradiation on HaCaT cells was significant higher than control group. Immunoblotting experiments confirmed the expression levels of AKT and Bad-p136 were significant increasing compared to normal expression of Bad protein after UVB irradiation. Therefore, a long time UVB radiation of 5 mJ/cm² may promote cell proliferation signaling pathway by Bad, which may be a potential risk factor for the development of skin tumors.

Key words: cell apoptosis, cell proliferation, ultraviolet UVB

中图分类号:

薛浙南, 黄欣, 曹迪, 陈瑾, 黄琨, 陈爱军. 低剂量UVB辐射对HaCaT细胞凋亡与增殖及Bad信号通路的影响[J]. 辐射防护, 2014, 34(3): 168-171.

Xue Zhenan, Huang Xin, Cao Di, Chen Jin, Huang Kun, Chen Aijun. Apoptosis in HaCaT and Bad Pathways[J]. RADIATION PROTECTION, 2014, 34(3): 168-171.

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