Effects of Sijunzi decoction on body weight and peripheral blood in 60Co γ irradiated mice
Li Chen, Shao Shuai, Tu Xumin, Fan Li, Qi Xuesong, Wang Chunyan,Tong Peng, Li Ning, Gou Qiao, Tian Mei, Qu Gonglin
RADIATION PROTECTION. 2015, 35(6):
345-350.
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CCK-8 Assay Kit was used to detect the survival rate of AHH-1 cells treated by 0, 5, 25, 50, 250, 500 μg/mL Sijunzi decoction and its single components for 48 h, or by 4 Gy γ-ray for 24 h after treatment with 0, 0.4, 2, 10, 50 μg/mL Sijunzi decoction and its single components. A total of 80 BALB/c mice female/male were randomly divided into 5 groups: control group without treatment, irradiation group, Sijunzi low-dose group (0.75 g/kg),Sijunzi middle-dose group (2.25 g/kg),Sijunzi high-dose group (6.75 g/kg). WBC, RBC, HGB, PLT counts post-irradiation 3 days and 7 days were examined by blood cell analyzer. Body weights before and after irradiation were also observed to investigate the effect of Sijunzi decoction on 3.5 Gy 60Co γ radiation-induced acute injury in mice. The results demonstrated that compared to control group cells (0 μg/mL), the survival rate of AHH-1 cells treated by 5, 25 and 50 μg/mL Sijunzi decoction for 48 h was not changed (p>0.05), but the survival rate of post-irradiation AHH-1 cells increased at 50 μg/mL Sijunzi decoction (p<0.05). After 3.5 Gy γ-ray, the WBC count of middle-dose Sijunzi decoction group increased compared to radiation group (p<0.01), moreover, the middle-dose Sijunzi decoction group can slow the PLT decline. Sijunzi groups also have good effect on the body weight regaining in mice after radiation.Sijunzi decoction had radiation-protective effect in post radiation AHH-1 cells. In addition, Sijunzi reduced the injury in irradiated mice through hematopoietic system rebuilt and body weight regain.
