海带多糖通过促进Sirt3/MnSOD表达来改善受照射后小鼠的下颌下腺损伤

辐射防护 ›› 2021, Vol. 41 ›› Issue (3): 264-270.

海带多糖通过促进Sirt3/MnSOD表达来改善受照射后小鼠的下颌下腺损伤

张思琴¹, 敖翩¹, 陈少勇¹, 柴溶¹, 王绍军², 韦力³, 徐杨¹, 陈晨¹

1.广西医科大学口腔医学院,南宁 530021;
2.广西壮族自治区妇幼保健院,南宁 530021;
3.广西医科大学人体解剖学教研室,南宁 530021

收稿日期:2020-08-18 出版日期:2021-05-20 发布日期:2021-07-12
通讯作者: 韦力。E-mail:weili@stu.gxmu.edu.cn
作者简介:张思琴(1991—),女,2016年7月毕业于咸宁医学院口腔医学专业,2019年7月毕业于广西医科大学口腔基础医学专业,获硕士学位,现为广西医科大学口腔医学专业博士研究生。E-mail:1144789235@qq.com
基金资助:
国家自然科学基金项目(81460479);广西自然科学基金项目(2018JJA140600);广西教育厅基金(ZD2014031)。

Laminaria Japonica Polysaccharide attenuates radiation-induced submandibular gland injury in mice via promoting Sirt3/MnSOD expression

ZHANG Siqin¹, AO Pian¹, CHEN Shaoyong¹, CHAI Rong¹, WANG Shaojun², WEI Li³, XU Yang¹, CHEN Chen¹

1. College of Stomatology, Guangxi Medical University, Nanning 530021;
2. Materna and Child Health Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021;
3. Department of Human Anatomy, Guangxi Medical University, Nanning 530021

Received:2020-08-18 Online:2021-05-20 Published:2021-07-12

摘要/Abstract

摘要： 为了评估海带多糖(laminaria japonica polysaccharide,LJP)对辐射诱导的唾液腺功能损伤的影响及其作用机制,对8周龄的雌性昆明小鼠给予⁶⁰Co γ射线(一次性单次照射,剂量15 Gy,剂量率1 250 cGy/min)照射和LJP干预,于照射后28 d进行唾液流量检测和苏木精-伊红染色,并通过免疫组化和RT-qPCR等实验方法来分析下颌下腺组织中与抗氧化应激有关的重要基因——沉默信息调节因子3 (Sirtuin3, Sirt3)和锰超氧化物歧化酶(Manganese superoxide dismutase, MnSOD)的表达。实验结果显示,LJP能改善受照射后小鼠的唾液流量和颌下腺结构的损伤,并上调其组织中Sirt3及MnSOD的表达。这表明LJP可能通过激活下颌下腺组织中Sirt3/MnSOD的表达来改善辐射诱导的下颌下腺功能损伤。

关键词: 海带多糖, 下颌下腺, 沉默信息调节因子3, 锰超氧化物歧化酶, 放射性口干

Abstract: The aim of this study is to investigate protective effect and the underlying mechanism of Laminaria Japonica Polysaccharide (LJP) on radiation-induced submandibular gland injury in mice. Eight-week-old female mice were randomly divided into four groups, including control group, LJP group, irradiation group, and irradiation plus LJP group. LJP (100 mg/kg/day) was intraperitoneally injected 3 days before irradiation and for consecutive 7 days (once daily).The mice from irradiation alone and irradiation plus LJP groups were exposed to a single dose of 15 Gy by using the ⁶⁰Co gamma rays. Histopathology and function of the submandibular glands (SMG) were detected on the 28th day post irradiation. The protein and mRNA expression of Sirt3 and MnSOD were respectively examined by real-time qPCR and immunohistochemical staining methods. Results showed that LJP injection significantly increased saliva flow rates in irradiated mice, improved the morphology and structure of SMG, and markedly up-regulated expressions of the Sirt3 and MnSOD. This suggests that LJP treatment could alleviate radiation-induced submandibular gland injury through activation of the Sirt3/MnSOD pathway.

Key words: Laminaria Japonica Polysaccharide, submandibular gland, Sirtuin3, manganese superoxide dismutase, radiation-induced xerostomia

中图分类号:

R142+.4

张思琴, 敖翩, 陈少勇, 柴溶, 王绍军, 韦力, 徐杨, 陈晨. 海带多糖通过促进Sirt3/MnSOD表达来改善受照射后小鼠的下颌下腺损伤[J]. 辐射防护, 2021, 41(3): 264-270.

ZHANG Siqin, AO Pian, CHEN Shaoyong, CHAI Rong, WANG Shaojun, WEI Li, XU Yang, CHEN Chen. Laminaria Japonica Polysaccharide attenuates radiation-induced submandibular gland injury in mice via promoting Sirt3/MnSOD expression[J]. RADIATION PROTECTION, 2021, 41(3): 264-270.

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