富氢水对60Co照射比格犬急性血液系统损伤的保护作用研究

辐射防护 ›› 2018, Vol. 38 ›› Issue (5): 428-432.

富氢水对⁶⁰Co照射比格犬急性血液系统损伤的保护作用研究

秦秀军, 孙鸽, 岳娟, 黄立群, 李炜宾, 尹晶晶, 李建国

中国辐射防护研究院药物毒理与放射损伤药物山西省重点实验室,太原 030006

收稿日期:2017-04-11 出版日期:2018-09-20 发布日期:2020-07-21
作者简介:秦秀军(1979—),男,2006年毕业于山西医科大学卫生毒理专业,副研究员。E-mail:qxj19982000@163.com
基金资助:
山西省实验动物专项资金项目(2013k08,2014k09)。

Protective effect of hydrogen rich water on acute hematological damage in Beagle dogs exposed to ⁶⁰Co

QIN Xiujun, SUN Ge, YUE Juan, HUANG Liqun, LI Weibin, YIN Jingjing, LI Jianguo

China Institute for Radiation Protection, Shanxi Key Laboratory of Drug Toxicology and Drug for Radiation Injury, Taiyuan 030006

Received:2017-04-11 Online:2018-09-20 Published:2020-07-21

摘要/Abstract

摘要： 本文研究了富氢水对⁶⁰Co γ 射线照射后Beagle犬急性血液系统损伤的保护作用。雄性Beagle犬随机分为对照组、单纯照射组和富氢水干预组。动物经单次2.0 Gy γ射线全身照射后,分别于第2天、第7天,采集动物外周血,检测血液学指标、抗氧化指标、淋巴细胞凋亡率及8-羟基脱氧鸟苷酸(8-OHdG)等。结果表明,在照后第7天,富氢水组红细胞计数(RBC)、血红蛋白含量(HGB)、红细胞压积(HCT)均高于单纯照射组(p＜0.01、p＜0.05、p＜0.05);在照后第2天、第7天,富氢水组血清中总抗氧化能力(T-AOC)均高于单纯照射组(p＜0.01,p＜0.05),丙二醛(MDA)均低于单纯照射组(p＜0.01,p＜0.05);在照后第2天、第7天,富氢水组淋巴细胞凋亡率均低于单纯照射组(p＜0.05,p＜0.01);在照后第7天,富氢水组8-OHdG浓度低于单纯照射组(p＜0.05)。以上结果说明,富氢水可通过抗氧化应激、抗凋亡机制改善照后Beagle犬血液系统损伤。

关键词: 富氢水, Beagle犬, γ射线, 血液系统, 辐射防护

Abstract: The protective effect of hydrogen rich water on the acute hematological damage of Beagle dogs exposed to ⁶⁰Co γ ray irradiation was studied. Male Beagle dogs were randomly divided into control group, irradiation group and hydrogen rich water intervention group. After a single irradiation of 2.0 Gy gamma ray, the peripheral blood of the animals was collected on the 2^nd and the 7^th day. The indexes of hematology, antioxidation, lymphocyte apoptosis rate and 8-OHdG were detected. The results showed that on the 7^th day after irradiation, RBC, HGB and HCT of the hydrogen rich water group were higher than those of the irradiation group (p<0.01, p<0.05, p<0.05); on the 2^nd day and the 7^th day after irradiation, T-AOC of the hydrogen rich water group was higher than that of the irradiated group (p<0.01, p<0.05), and MDA was lower than that of the irradiation group (p<0.01, p<0.05); on the 2^nd day and the 7^th day after irradiation, lymphocyte apoptosis rate of the hydrogen rich water group was lower than that of the irradiation group (p<0.05, p<0.01); on the 7^th day after exposure, the concentration of 8-OHdG in hydrogen rich water group was lower than that of the irradiation group (p<0.05). These results indicate that hydrogen rich water can improve the blood index of Beagle dogs by the mechanism of anti oxidative stress and anti apoptosis.

Key words: hydrogen rich water, Beagle dog, gamma ray, hematological parameter, radiation protection

中图分类号:

Q691

秦秀军, 孙鸽, 岳娟, 黄立群, 李炜宾, 尹晶晶, 李建国. 富氢水对⁶⁰Co照射比格犬急性血液系统损伤的保护作用研究[J]. 辐射防护, 2018, 38(5): 428-432.

QIN Xiujun, SUN Ge, YUE Juan, HUANG Liqun, LI Weibin, YIN Jingjing, LI Jianguo. Protective effect of hydrogen rich water on acute hematological damage in Beagle dogs exposed to ⁶⁰Co[J]. RADIATION PROTECTION, 2018, 38(5): 428-432.

参考文献

[1] Vijayalaxmi, Reiter R J, Tan D X, et al. Melatonin as a radioprotective agent: A review [J]. Int J Radiat Oncol Biol Phys, 2004, 59(3): 639-653.
[2] Ohsawa I, Ishikawa M, Takahashi K, et al. Hydrogen acts as a therapeutic antioxidam by selectively reducing cytotoxic oxygen radicals [J]. Nat Med, 2007, l3: 688-694.
[3] Ohta S, Nakao A, Ohno K. The 2011 medical molecular hydrogen symposium: An inaugural symposium of the journal medical gas research [J]. Med Gas Res, 2011, 1(1): 10-16.
[4] Yasuhiro Terasaki, Ikuroh Ohsawa, Mika Terasaki, et al, Hydrogen therapy attenuates irradiation-induced lung damage by reducing oxidative stress [J]. Am J Physiol Lung Cell Mol Physiol, 2011, 301(4): 415-426.
[5] QIAN L, CAO F, CUI J, et al. The potential cardioprotective effects of hydrogenin irradiated mice [J]. J Radiat Res (Tokyo), 2010, 51(6): 741-747.
[6] 秦秀军, 尹晶晶, 许文黎, 等. 不同剂量富氢水对小鼠辐射损伤保护作用的研究 [J]. 中华放射医学与防护杂志, 2014, 34(8): 578-582.
QIN Xiujun, YIN Jingjing, XU Wenli, et al. Protective effects of different dose of hydrogen-rich water on radiation injury in mice [J]. Chin J Radiol Med Prot, 2014, 34(8): 578-582.
[7] ZHANG Junling, XUE Xiaolei, HAN Xiaodan, et al. Hydrogen-rich water ameliorates total body irradiation-induced hematopoietic stem cell injury by reducing hydroxyl radical [J]. Oxid Med Cell Longev, 2017, 3(1): 1-16.
[8] 秦秀军, 田宇, 安全, 等. 富氢水对小鼠辐射损伤的保护作用 [J]. 辐射防护通讯, 2013, 33(4): 28-33.
QIN Xiujun, TIAN Yu, AN Quan, et al. Study on the protective effects of hydrogen-rich water given against radiation injury in mice [J]. Radiation Protection Bulletin, 2013, 33(4): 28-33.
[9] QIAN L R, LI B L, CAO F, et al. Hydrogen-rich PBS protects cultured human cells from ionizing radiation-induced cellular damage [J]. Nuclear Technology & Radiation Protection, 2010, 25(1): 23-29.
[10] Tomoko N, Tomomi K, Yukio M, et al. Maternal molecular hydrogen administration on lipopolysaccharide-induced mouse fetal brain injury [J]. J Clin Biochem Nutr, 2015, 57(3): 15-90.
[11] Ryosuke Kurokawa, Tomoki Seo, Bunpei Sato, et al. Convenient methods for ingestion of molecular hydrogen: drinking, injection, and inhalation [J]. Med Gas Res, 2015, 5(5): 13-20.

富氢水对⁶⁰Co照射比格犬急性血液系统损伤的保护作用研究

Protective effect of hydrogen rich water on acute hematological damage in Beagle dogs exposed to ⁶⁰Co

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