电离辐射对人肝细胞MXR7基因表达的影响及放射性工作人员外周血MXR7的表达分析

辐射防护 ›› 2018, Vol. 38 ›› Issue (6): 517-521.

电离辐射对人肝细胞MXR7基因表达的影响及放射性工作人员外周血MXR7的表达分析

党旭红^1,2, 王放³, 左雅慧¹, 原雅艺¹, 张景云¹, 董娟聪¹, 张忠新¹, 段志凯¹

1.中国辐射防护研究院,太原 030006;
2.中国原子能科学研究院,北京 102413;
3.长治医学院附属和平医院,山西长治 046000

收稿日期:2018-01-12 出版日期:2018-11-20 发布日期:2020-07-21
通讯作者: 左雅慧。E-mail:yahuiz@163.com
作者简介:党旭红(1983—),男,2009年毕业于山西大学生物化学与分子生物学专业,获理学硕士学位,副研究员。E-mail:dangxuhong005@163.com
基金资助:
山西省应用基础研究项目青年科技研究基金(201601D202056)。

Detection and analysis of MXR7 gene expression in human liver cells induced by radiation and peripheral blood of radiation workers

DANG Xuhong^1,2, ZUO Yahui¹, WANG Fang³, ZHANG Jingyun¹, DONG Juancong¹, ZHANG Zhongxin¹, DUAN Zhikai¹

1. China Institute for Radiation Protection, Taiyuan 030006;
2. China Institute of Atomic Energy, Beijing 102413;
3. Heping Hospital Affiliated to Changzhi Medical College, Shanxi Changzhi 046000

Received:2018-01-12 Online:2018-11-20 Published:2020-07-21

摘要/Abstract

摘要： 为探讨电离辐射对人正常肝细胞及外周血MXR7基因表达的影响,利用⁶⁰Co γ射线对培养的人肝细胞HL-7702进行照射,同时,抽取某工厂放射性工作人员外周血,通过实时荧光定量PCR对照射后的各剂量组肝细胞及放射性工作人员外周血MXR7基因的表达情况进行检测分析。结果表明,电离辐射可诱导人正常肝细胞MXR7基因的差异表达,且基因表达与照射剂量存在一定的剂量相关性;放射性工作人员外周血MXR7基因表达与非放射性工作人员相比显著升高。

关键词: 人正常肝细胞, 放射性工作人员, MXR7基因, 电离辐射, 外周血

Abstract: In order to explore the changes of MXR7 genes expression in human liver cells HL-7702 and peripheral blood induced by radiation,the expression changes of MXR7 gene in human liver cells irradiated by ⁶⁰Co γ-ray were detected by real-time fluorescent quantitative PCR. Furthermore, MXR7 gene expression levels were detected in peripheral blood from radiation workers. Results showed that ionizing radiation can induce the change of MXR7 genes expression in human liver cells, and it is dose-related. The expression of MXR7 gene in peripheral blood from radiation workers was up-regulated to compare with the expression of non-radiation workers.

Key words: human liver cell HL-7702, radiation workers, MXR7 gene, ionizing radiation, peripheral blood

中图分类号:

Q691

党旭红, 王放, 左雅慧, 原雅艺, 张景云, 董娟聪, 张忠新, 段志凯. 电离辐射对人肝细胞MXR7基因表达的影响及放射性工作人员外周血MXR7的表达分析[J]. 辐射防护, 2018, 38(6): 517-521.

DANG Xuhong, ZUO Yahui, WANG Fang, ZHANG Jingyun, DONG Juancong, ZHANG Zhongxin, DUAN Zhikai. Detection and analysis of MXR7 gene expression in human liver cells induced by radiation and peripheral blood of radiation workers[J]. RADIATION PROTECTION, 2018, 38(6): 517-521.