137Cs-γ辐照对拟南芥子一代的影响研究

摘要/Abstract

摘要： 为评价核设施对陆生生态系统的辐射影响,探讨γ辐射对植物种群的辐射效应,分析γ射线辐照引起子一代植物的诱变效应和诱变机制,选取哥伦比亚生态型拟南芥进行不同剂量率¹³⁷Cs-γ辐照,并测定辐照后拟南芥子一代生长参数、生理生化指标和荧光参数。结果表明:不同剂量率γ辐照后,子一代拟南芥生长发育存在差异,株高整体呈现随剂量率的增加先升高后降低的趋势,50 mGy/h辐照后的拟南芥子一代叶片最小,丙二醛含量、过氧化物酶比活力、有效光化学量子产量最低,20 mGy/h的超氧化物歧化酶和过氧化氢酶比活力最低,谷胱甘肽还原酶、脯氨酸表现为辐照后的拟南芥子代植株均显著低于对照组,10 mGy/h、50 mGy/h辐照后叶绿素a和叶绿素b含量显著低于其他处理组,非光淬灭系数整体表现出随剂量率的增加呈先降低后升高的趋势,表明低剂量率辐照未对拟南芥子代光合作用反应中心PS Ⅱ造成不可逆伤害,植株主要通过调控抗氧化酶系统和渗透调节物质,对电离辐射做出应答。

关键词: ¹³⁷Cs-γ辐照, 拟南芥, 子一代, 生理指标, 荧光参数

Abstract: In order to evaluate the radiation impact of nuclear facilities on terrestrial ecosystems, and to explore the effects and biological mechanisms of γ radiation on the offspring of Arabidopsis thaliana, the Col-0 Arabidopsis thaliana was selected and irradiated at different dose rates (0 mGy/h, 10 mGy/h, 20 mGy/h, 50 mGy/h, 100 mGy/h). The plant height, leaf length, leaf width, chlorophyll content, superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), glutathione reductase (GR), malondialdehyde (MDA), proline (PRO), and chlorophyll fluorescence indicators were measured. The results indicated that there were differences in the growth and development of Arabidopsis thaliana progeny after irradiation. The overall plant height showed a trend of increasing first and then decreasing. Plant leaves, MDA, POD, and effective photochemical quantum yield were the minimum points after 50 mGy/h irradiation. The SOD and CAT specific activity were the lowest at 20 mGy/h. GR and PRO showed that the irradiated Arabidopsis thaliana offspring plants were significantly lower than the control group. The content of chlorophyll a and chlorophyll b in 10 mGy/h and 50 mGy/h irradiation was significantly lower than other treatments. The non-photoquenching coefficient tendency indicates that low-dose rate irradiation did not cause irreversible damage to the photosynthetic response center PS Ⅱ in the offspring of Arabidopsis thaliana. Plants mainly respond to ionizing radiation by regulating the antioxidant enzyme system and osmolyte substances.

Key words: γ irradiation, Arabidopsis thaliana, first generation, physiological indicators, fluorescence parameters

中图分类号:

X591

原寒, 乔新燕, 曹少飞, 李建国, 于志翔. ¹³⁷Cs-γ辐照对拟南芥子一代的影响研究[J]. 辐射防护, 2025, 45(4): 419-427.

YUAN Han, QIAO Xinyan, CAO Shaofei, LI Jianguo, YU Zhixiang. Effects of ¹³⁷Cs γ irradiation on Arabidopsis thaliana progeny[J]. RADIATION PROTECTION, 2025, 45(4): 419-427.

参考文献

[1] Lee M H, Moon, R Y, et al. Practical use of chemical probes for reactive oxygen species produced in biological systems by gamma-irradiation[J]. Radiation Physics & Chemistry, 2009, 78(5): 323-327.
[2] Geras'kin S, Evseeva T, Oudalova A. Effects of long-term chronic exposure to radionuclides in plant populations[J]. Journal of Environmental Radioactivity, 2013, 121: 22-32.
[3] Gupta D K, Vuković A, Semenishchev V S, et al. Uranium accumulation and its phytotoxicity symptoms in Pisum sativum L[J]. Environmental Science and Pollution Research International, 2020, 27(3): 3513-3522.
[4] 刘素纯, 萧浪涛, 廖柏寒, 等. 铅胁迫对黄瓜幼苗抗氧化酶活性及同工酶的影响[J]. 应用生态学报, 2006, 17(2): 300-304.
LIU Suchun, XIAO Langtao, LIAO Baihan, et al. Effects of Lead stress on anti-oxidative enzyme activities and isoenzymes in cucumber seedlings[J]. Chinese Journal of Applied Ecology, 2006, 17(2): 300-304.
[5] 张福锁.环境胁迫与植物根际营养[M]. 北京: 中国农业出版社, 1998.
ZHANG Fusuo. Environmental stress and plant rhizosphere nutrition[M]. Beijing:China Agriculture Press,1998.
[6] 王龙, 樊婕, 魏畅, 等. 外源抗坏血酸对铜胁迫菊苣幼苗生长的缓解效应[J]. 草业学报, 2021, 30(4): 150-159.
WANG Long, FAN Jie, WEI Chang, et al. Mitigative effect of exogenous ascorbic acid on the growth of copper-stressed chicory (Cichorium intybus) seedlings[J]. Acta Prataculturae Sinica, 2021, 30(4): 150-159.
[7] 林源秀, 顾欣昕, 汤浩茹. 植物谷胱甘肽还原酶的生物学特性及功能[J]. 中国生物化学与分子生物学报, 2013, 29(6): 534-542.
LIN Yuanxiu, GU Xinxin, TANG Haoru. Characteristics and biological functions of glutathione reductase in plants[J]. Chinese Journal of Biochemistry and Molecular Biology, 2013, 29(6): 534-542.
[8] 张林, 陈翔, 吴宇, 等. 脯氨酸在植物抗逆中的研究进展[J]. 江汉大学学报(自然科学版), 2023, 51(1): 42-51.
ZHANG Lin, CHEN Xiang, WU Yu, et al. Research progress of proline in plant stress resistance[J]. Journal of Jianghan University Natural Science Edition, 2023, 51(1): 42-51.
[9] 吴娟, 施国新, 夏海威, 等. 外源钙对汞胁迫下菹草(Potamogeton crispus L.)叶片抗氧化系统及脯氨酸代谢的调节效应[J]. 生态学杂志, 2014, 33(2): 380-387.
WU Juan, SHI Guoxin, XIA Haiwei, et al. Effects of exogenous calcium on antioxidant system and proline metabolism of Potamogeton crispus L. leaves under mercury stress[J]. Chinese Journal of Ecology, 2014, 33(2): 380-387.
[10] O'Brien J A, Daudi A, Butt V S, et al. Reactive oxygen species and their role in plant defence and cell wall metabolism[J]. Planta, 2012, 236(3): 765-779.
[11] CHEN Qiang, ZHANG Meide, SHEN Shihua. Effect of salt on malondialdehyde and antioxidant enzymes in seedling Roots of Jerusalem artichoke (Helianthus tuberosus L.)[J]. Acta Physiologiae Plantarum, 2011, 33(2): 273-278.
[12] EI B H, Ahmed O K, EI D W. Effect of low dose γ-irradiation on oxidative stress and secondary metabolites production of rosemary(Rosmarinus officinalis L.) callus culture[J]. Radiation Physics & Chemistry, 2011, 80: 968-976.
[13] 雷晓, 强继业, 陈云飞. ⁶⁰Co-γ射线辐射对一品红丙二醛及几种保护酶的影响[J]. 云南农业大学学报, 2006, 21(6): 855-858.
LEI Xiao, QIANG Jiye, CHEN Yunfei. Effects of radiation dosages of ⁶⁰Co-γ ray on some cell protective enzymes and malondialdehyde of the euphorbia pulcherrima[J]. Journal of Yunnan Agricultural Unversity, 2006, 21(6): 855-858.
[14] Bourne-Worster S, Feighan O, Manby F R. Charge transfer as a mechanism for chlorophyll fluorescence concentration quenching[J]. Proceedings of the National Academy of Sciences of the United States of America, 2023, 120(5): e2210811120.
[15] 郭少玲, 高健, 徐有明, 等. ⁶⁰Co γ辐射对毛竹种子萌发过程中内源激素含量的影响[J]. 中国农学通报, 2013, 29(25): 26-31.
GUO Shaoling, GAO Jian, XU Youming, et al. Effect of ⁶⁰Co γ rays radiation on Endogenous Hormone during seed germination[J]. Chinese Agricultural Science Bulletin, 2013, 29(25): 26-31.
[16] 许银莲, 强继业. ⁶⁰Co-γ射线辐射对粉掌铁兰某些生理指标的影响[J]. 种子学报, 2005, 24(4): 26-27, 30.
XU Yinlian, QIANG Jiye. Effects of ⁶⁰Co-gamma-ray radiation on some physiological indexes of C. palmata[J]. Journal of Seed Science, 2005, 24(4): 26-27, 30.
[17] 高璐, 李晓锋, 董言笑, 等. 不结球白菜光合作用及叶绿素荧光参数分析[J/OL]. 分子植物育种, 1-8[2025-01-22]. http://kns.cnki.net/kcms/detail/46.1068.S.20230331.1126.010.html.
[18] 闵锐. 低剂量/低剂量率电离辐射生物效应[J]. 辐射研究与辐射工艺学报, 2014, 32(6): 1-9.
MIN Rui. Biological effects induced by low dose/low dose rate ionizing radiation[J]. Journal of Radiation Research and Radiation Processing, 2014, 32(6): 1-9.
[19] MIAO Renhui, MIAO Yuan, LIU Yuli, et al. Late-season drought exerts more negative effects on plant diversity and cover than early-season drought through changing soil moisture in a semi-arid grassland[J]. Journal of Vegetation Science, 2022, 33(5): e13151.
[20] Gruber B D, Giehl R F H, Friedel S, et al. Plasticity of the Arabidopsis root system under nutrient deficiencies[J]. Plant Physiology, 2013, 163(1): 161-179.
[21] Singh B, Datta P S. Effect of low dose gamma irradiation on plant and grain nutrition of wheat[J]. Radiation Physics and Chemistry, 2010, 79(8): 819-825.
[22] LIU Yonglin, LIU Yingpin, CHEN Yu, et al. Sulfur fertiliser enhancement of Erigeron breviscapus (Asteraceae) quality by improving plant physiological responses and reducing soil cadmium bioavailability[J]. Environmental Science and Pollution Research International, 2022, 29(46): 70508-70519.
[23] CHEN Siting, QIU Guanglong. Overexpression of the intertidal seagrass J protein ZjDjB1 enhances tolerance to chilling injury[J]. Plant Biotechnology Reports, 2022, 16(4): 419-435.
[24] CHEN Zhanyu, YANG Xiaoqin, TANG Minghao, et al. Molecular characterization and drought resistance of GmNAC3 transcription factor in Glycine max (L.) Merr[J]. International Journal of Molecular Sciences, 2022, 23(20): 12378.
[25] ICRP. Environmental protection: the concept and use of reference animals and plants[R]. ICRP Report 108.Oxford: Pergamon Press, 2008.
[26] 章铁, 刘秀清, 张金良, 等. 不同剂量率⁶⁰Co-γ射线低剂量辐射对小麦农艺性状的影响[J]. 中国农学通报, 2008, 24(1): 220-223.
ZHANG Tie, LIU Xiuqing, ZHANG Jinliang, et al. Effect of ⁶⁰Co -γ ray low dose radiation on agronomic traits of wheat with different dose-rate[J]. Chinese Agricultural Science Bulletin, 2008, 24(1): 220-223.
[27] Goh E J, Kim J B, Kim W J, et al. Physiological changes and anti-oxidative responses of Arabidopsis plants after acute and chronic γ-irradiation[J]. Radiation and Environmental Biophysics, 2014, 53(4): 677-693.
[28] 李波, 李祥莉, 赵宇佳, 等. ⁶⁰Co-γ射线对无芒雀麦种子的辐照效应[J]. 云南农业大学学报:自然科学, 2017, 32(5): 756-761.
LI Bo, LI Xiangli, ZHAO Yujia, et al. Effect of ⁶⁰Co-γ ray irradiation on brome seed[J]. Journal of Yunnan Agricultural University, 2017, 32(5): 756-761.
[29] 王振搏, 刘红恩, 许嘉阳, 等. 镉胁迫下施硒对冬小麦幼苗镉吸收、抗氧化能力及植株超微结构的影响[J]. 植物营养与肥料学报, 2023, 29(3): 530-543.
WANG Zhenbo, LIU Hongen, XU Jiayang, et al. Effects of selenium application on cadmium uptake, antioxidant activity, and ultrastructure of winter wheat seedlings under cadmium stress[J]. Plant Nutrition and Fertilizer Science, 2023, 29(3): 530-543.
[30] 曲丹阳, 顾万荣, 李丽杰, 等. 壳聚糖对镉胁迫下玉米幼苗叶片AsA-GSH循环的调控效应[J]. 植物科学学报, 2018, 36(2): 291-299.
QU Danyang, GU Wanrong, LI Lijie, et al. Regulation of chitosan on the ascorbate-glutathione cycle in Zea mays seedling leaves under cadmium stress[J]. Plant Sclence Journal, 2018, 36(2): 291-299.
[31] 罗维纲, 张晋龙, 刘云根. 砷在不同物候期香蒲中的分布及其生理影响[J]. 中国环境监测, 2023, 39(6): 133-141.
LUO Weigang, ZHANG Jinlong, LIU Yungen. Subcellular distribution and physiological response of arsenic in typha angustifolius L.at different phenological stages[J]. Environmental Monitoring in China, 2023, 39(6): 133-141.
[32] 高志民, 刘颖丽, 彭镇华. 毛竹PSⅡ大量捕光天线蛋白基因克隆及其表达分析[J]. 植物科学学报, 2012, 30(1): 64-71.
GAO Zhimin, LIU Yingli, PENG Zhenhua. Cloning and molecular characterization of the major light harvesting antenna protein genes of PS Ⅱ in Phyllostachys edulis[J]. Journal of Wuhan Botanical Research, 2012, 30(1): 64-71.
[33] 牛贺雨, 刘丹, 彭梦婕, 等. ⁶⁰Co-γ辐射对大花紫薇叶绿素荧光特性的影响[J]. 东北林业大学学报, 2020, 48(1): 23-28.
NIU Heyu, LIU Dan, PENG Mengjie, et al. Effect of ⁶⁰Co-γ radiation on chlorophyll fluorescence characteristics of lagerstroemia speciosa Pers[J]. Journal of Northeast Forestry University, 2020, 48(1): 23-28.
[34] 李玉明, 赵春生, 乔蓬蕾, 等. 60Co-γ射线辐射西瓜种子对叶片光合色素含量和气体交换参数的影响[J]. 中南农业科技, 2023, 44(8): 17-20.
LI Yuming, ZHAO Chunsheng, QIAO Penglei, et al. Effects of 60co-gamma radiation on photosynthetic pigment content and gas exchange parameters in watermelon seeds[J]. South-Central Agricultural Science and Technology, 2023, 44(8): 17-20.
[35] 桂仁意, 刘亚迪, 郭小勤, 等. 不同剂量¹³⁷Cs-γ射对毛竹幼苗叶片叶绿素荧光参数的影响[J]. 植物学报, 2010, 45(1): 66-72.
[36] 鲁松. 叶绿素荧光动力学在植物抗逆性研究中的应用[J]. 四川林业科技, 2013, 34(4): 69-71.
LU Song. Application of chlorophyll fluorescence kinetics in researches on plant stress resistance[J]. Journal of Sichuan Forestry Science and Technology, 2013, 34(4): 69-71.
[37] 范菁, 黄建中, 徐洁, 等. ⁶⁰Co-γ辐射对茭白叶片光合特性和荧光参数的影响[J]. 长江蔬菜, 2011(16): 36-39.
FAN Jing, HUANG Jianzhong, XU Jie, et al. Effects of ⁶⁰Co-γ ray irradiation on photosynthetic characteristics and chlorophyll fluorescence parameters in leaves of Zizania latifolia[J]. Journal of Changjiang Vegetables, 2011(16): 36-39.

¹³⁷Cs-γ辐照对拟南芥子一代的影响研究

Effects of ¹³⁷Cs γ irradiation on Arabidopsis thaliana progeny

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 10

[1]	陈佳辰, 王彦, 廉冰, 杨洁, 岳琪, 蒙滨驰. 放射性粉末物料洒落事故气溶胶分布特性CFD研究[J]. 辐射防护, 2024, 44(5): 562-572.
[2]	邱继林, 李毅, 俞杰, 田林涛, 张渊, 王佳, 韩明, 张治权, 沈大鹏, 王瑞云. 椭圆型碘吸附器设计与性能分析[J]. 辐射防护, 2024, 44(5): 524-529.
[3]	陈佳辰, 王彦, 廉冰, 杨洁, 岳琪, 武翡翡, 蒙滨驰. 简述安全报告系列第19号(SRS 19)修订版中筛选系数的变化[J]. 辐射防护, 2023, 43(1): 72-76.
[4]	汪弘, 何润程, 刘永, 洪昌寿, 李向阳, 罗梦柯, 王建敏. 预制裂隙下铀尾矿库土质覆盖层氡析出实验研究[J]. 辐射防护, 2022, 42(6): 603-610.
[5]	梁国帅, 陈柏迪, 陈志东, 邓飞. 土壤中²³⁸U、²²⁶Ra、²¹⁰Pb、²¹⁰Po在3种蔬菜中的转移及食用后剂量估算[J]. 辐射防护, 2021, 41(3): 229-236.
[6]	孙祁, 盖文佳, 江灏, 闫洋洋, 黄欣杰, 梁云, 祝娇, 苏默龙. 地下工程降氡技术的应用与现状[J]. 辐射防护, 2020, 40(5): 462-469.
[7]	肖建华, 张晓文, 杨蓉, 杜仁铠, 张德. 铀浓缩厂放射性废物管理与最小化实践[J]. 辐射防护, 2019, 39(1): 61-66.
[8]	范红艳, 王艳春, 任旷, 李曼, 徐玉良, 钟秀宏, 刘师兵, 吕士杰. 鹿茸多肽对微波辐射后小鼠学习记忆的影响研究[J]. 辐射防护, 2018, 38(1): 51-57.
[9]	叶勇军, 代鑫涛, 丁德馨, 江俊廷, 李志. 含氡放射性水体氡析出规律的理论研究[J]. 辐射防护, 2017, 37(1): 56-61.
[10]	段中山, 袁涛, 冯孝杰, 秦兵, 曾晨浩. 地铁站内脏弹爆炸烟团扩散的仿真研究[J]. 辐射防护, 2016, 36(5): 279-284.
[11]	李洋, 杨洁, 廉冰, 赵杨军, 陈海龙, 劳业程. 我国含铀氟化钙渣用于水泥生产的辐射环境影响研究[J]. 辐射防护, 2015, 35(S1): 48-52.
[12]	杨洁, 李洋, 廉冰, 赵杨军, 陈海龙. ¹⁴C辐射剂量评价模式中稳定碳份额研究[J]. 辐射防护, 2015, 35(S1): 58-62.
[13]	陈琪萍, 何佳恒, 马宗平, 王关全, 刘国平. 富勒烯衍生物对X射线所致小鼠肝线粒体损伤的保护作用[J]. 辐射防护, 2015, 35(4): 214-220.
[14]	岳琪, 廉冰, 王彦, 杨洁, 陈佳辰, 蒙滨驰, 武翡翡. 核电厂C-14液态流出物排放所致公众剂量筛选评价研究[J]. 辐射防护, 2024, 44(6): 601-611.
[15]	凌辉, 夏子通, 赵伟, 马明清, 吴鹏. ²³⁸U、²³²Th、²²⁶Ra、¹³⁷Cs在北山合头草中的转移系数研究[J]. 辐射防护, 2025, 45(4): 410-418.