橙皮苷和橙皮素对电离辐射致小鼠心血管损伤的防护作用研究

摘要/Abstract

摘要： 本研究以C57BL/6J小鼠为对象,探讨橙皮苷和橙皮素对其电离辐射致心血管损伤的防护作用。将小鼠随机分为空白对照组(NC组)、照射对照组(IR组)、橙皮苷低剂量组(L组200 mg/kg)、橙皮苷中剂量组(M组400 mg/kg)、橙皮苷高剂量组(H组800 mg/kg)和橙皮素低剂量组(L组50 mg/kg)、橙皮素中剂量组(M组100 mg/kg)、橙皮素高剂量组(H组200 mg/kg)8个组。照前2 h采用灌胃方式,空白对照组和照射对照组给予等量溶媒,除空白对照组外,各组小鼠均接受单次2 Gy的⁶⁰Co γ射线照射。分别于照射后24 h和48 h,对小鼠的免疫、抗氧化和DNA损伤三方面指标进行检测。结果显示,与照射组相比,给予橙皮苷和橙皮素的小鼠的肝脏指数均显著降低,但未表现出明显的剂量-效应关系,说明橙皮苷和橙皮素可能对辐射损伤小鼠的器官具有一定的保护作用;给予橙皮苷和橙皮素的小鼠血清中IL-1β、IL-6等炎性因子均显著降低,且具有剂量-效应关系,高剂量组表现更突出;给予橙皮苷和橙皮素均可显著降低小鼠主动脉丙二醛(MDA)含量和过氧化氢(H₂O₂)含量,表明两种处理具有显著的抗氧化作用;给予橙皮苷和橙皮素能降低照射后小鼠主动脉组织γ-H2AX foci数量,且具有剂量依赖性关系,表明两种处理能减轻照射后小鼠主动脉DNA的损伤。本研究的结果表明橙皮苷和橙皮素对照射致小鼠血管损伤具有一定的保护作用。

关键词: 橙皮苷, 橙皮素, 电离辐射, DNA损伤, 辐射防护

Abstract: This study investigates the protective effects of hesperidin and hesperetin on radiation-induced cardiovascular injury in C57BL/6J mice. The mice were randomly divided into eight groups: a blank control group (NC group), an irradiated control group (IR group), a hesperidin low-dose group (L group, 200 mg/kg), a hesperidin medium-dose group (M group, 400 mg/kg), a hesperidin high-dose group (H group, 800 mg/kg), a hesperetin low-dose group (L group, 50 mg/kg), a hesperetin medium-dose group (M group, 100 mg/kg), and a hesperetin high-dose group (H group, 200 mg/kg). Two hours before irradiation, the blank control group and the irradiated control group were given an equal amount of solvent by gavage method. Except for the blank control group, all groups of mice were exposed to a single dose of 2 Gy of ⁶⁰Co γ-ray irradiation. At 24 h and 48 h after irradiation, indices related to immunity, antioxidant capacity, and DNA damage in the mice were measured. The results showed that compared with the irradiated group, the liver index of the mice given hesperidin and hesperetin was significantly reduced, without a clear dose-effect relationship, indicating that hesperidin and hesperetin may have a certain protective effect on the organs of irradiated mice. The levels of pro-inflammatory factors such as IL-1β and IL-6 in the serum of mice given hesperidin and hesperetin were significantly decreased and showed a dose-effect relationship, with the high-dose groups being more prominent. The administration of hesperidin and hesperetin significantly reduced the content of malondialdehyde (MDA) and hydrogen peroxide (H₂O₂) in the aorta of the mice, indicating that both treatments have significant antioxidant effects. The administration of hesperidin and hesperetin reduced the number of γ-H2AX foci in the aortic tissue after irradiation in a dose-dependent manner, indicating that both treatments can alleviate the DNA damage in the mouse aorta after irradiation. The results of this study suggest that hesperidin and hesperetin have a certain protective effect against radiation-induced vascular damage in mice, and the possibility of their development as health supplements requires further exploration.

Key words: hesperidin, hesperetin, ionizing radiation, DNA damage, radiation protection

中图分类号:

Q691.5

胡伟翔, 赵红玲, 张瑜, 马岚芳, 周平坤, 关华. 橙皮苷和橙皮素对电离辐射致小鼠心血管损伤的防护作用研究[J]. 辐射防护, 2024, 44(3): 304-314.

HU Weixiang, ZHAO Hongling, ZHANG Yu, MA Lanfang, ZHOU Pingkun, GUAN Hua. Study on the protective effect of hesperidin and hesperetin on radiation-induced cardiovascular injury in mice[J]. RADIATION PROTECTION, 2024, 44(3): 304-314.

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