川陕花椒根皮提取物对人角质形成细胞辐射损伤的防护作用

辐射防护 ›› 2023, Vol. 43 ›› Issue (6): 657-664.

川陕花椒根皮提取物对人角质形成细胞辐射损伤的防护作用

王成芳¹, 齐雪松¹, 邵帅¹, 杜树山², 苟巧¹

1.中国疾病预防控制中心辐射防护与核安全医学所,辐射防护与核应急中国疾病预防控制中心重点实验室,北京 100088;
2.北京师范大学地理科学学部,北京 100875

收稿日期:2023-03-07 出版日期:2023-11-20 发布日期:2023-12-25
通讯作者: 苟巧。E-mail:gouqiao@nirp.chinacdc.cn
作者简介:王成芳(1983—),女,2007年毕业于辽宁中医药大学,获中药学学士学位,2010年毕业于辽宁中医药大学,获生药学硕士学位,2013年毕业于北京师范大学,获理学博士学位,副研究员。E-mail: wangchengfang@nirp.chinacdc.cn

Protective effects of extract from the root bark of Zanthoxylum piasezkii on radiation injury of human keratinocytes

WANG Chengfang¹, QI Xuesong¹, SHAO Shuai¹, DU Shushan², GOU Qiao¹

1. National Institute for Radiological Protection, Chinese Centre for Disease Control and Prevention;Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC,Beijing 100088, China;
2. Beijing Normal University,Faculty of Geographical Science,Beijing 100875

Received:2023-03-07 Online:2023-11-20 Published:2023-12-25

摘要/Abstract

摘要： 放射性皮肤损伤是接受放射治疗的患者常见的并发症,其发病机制复杂,患者个体化差异较大,临床上防治药物疗效良莠不齐,因而积极开发防治放射性皮肤损伤的药物仍十分必要。花椒是中医临床上用于治疗皮肤疾病的常用药,本文研究川陕花椒根皮提取物(ZPE)对人皮肤角质形成细胞HaCaT的辐射防护作用,并初步探讨其机制。研究结果显示,2 mg/mL ZPE对8.0 Gy γ射线照射引起的HaCaT细胞损伤具有防护作用,可减少细胞凋亡和坏死,其作用机制可能与降低细胞炎症因子IL-1β和IL-6的分泌、细胞内ROS水平以及p38和JNK蛋白磷酸化水平有关。本文的研究可为放射性皮肤损伤防治药物的研发提供理论依据。

关键词: 放射性皮肤损伤, 川陕花椒, 辐射防护, 人角质形成细胞

Abstract: Radiation-induced skin injury is a common complication of patients receiving radiotherapy. Its pathogenesis is complicated and varies greatly with individual patients. Therefore, it is necessary to develop the drugs for prevention and treatment of radiation-induced skin injury. Chinese prickly ash (Zanthoxylum bungeanum) is a common medicine used for skin diseases in traditional Chinese medicine. In this study, the radio-protective effect of the root bark extract of Zanthoxylum piasezkii (ZPE) on HaCaT was investigated and its mechanism was preliminary discussed. The results showed that 2 mg/mL ZPE could protect HaCaT cells from damage induced by 8.0 Gy γ ray irradiation, and could reduce apoptosis and necrosis of HaCaT cells. The mechanism may be related to the reduction of the secretion of IL-1β and IL-6, the level of ROS and the phosphorylation of p38 and JNK proteins. This work provides a theoretical basis for the development of preventive and therapeutic drugs for radiation-induced skin injury.

Key words: Radiation-induced skin injury, Zanthoxylum piasezkii, radiation protection, human keratinocytes

中图分类号:

R818

王成芳, 齐雪松, 邵帅, 杜树山, 苟巧. 川陕花椒根皮提取物对人角质形成细胞辐射损伤的防护作用[J]. 辐射防护, 2023, 43(6): 657-664.

WANG Chengfang, QI Xuesong, SHAO Shuai, DU Shushan, GOU Qiao. Protective effects of extract from the root bark of Zanthoxylum piasezkii on radiation injury of human keratinocytes[J]. RADIATION PROTECTION, 2023, 43(6): 657-664.

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