两种海洋生物多糖对60Co辐射损伤小鼠保护作用的研究

辐射防护 ›› 2019, Vol. 39 ›› Issue (3): 242-248.

两种海洋生物多糖对⁶⁰Co辐射损伤小鼠保护作用的研究

于涛¹, 王蕾¹, 纪建达¹, 杨青川¹, 张飞¹, 许晨², 许建中²

1.自然资源部第三海洋研究所,海洋放射性技术与环境安全评估实验室,福建厦门 361005;
2.自然资源部第三海洋研究所,海洋生物遗传资源重点实验室,福建厦门 361005

收稿日期:2018-11-22 出版日期:2019-05-15 发布日期:2019-10-29
作者简介:于涛(1971—),女,1994年6月毕业于成都理工学院工业分析专业,1997年6月毕业于成都理工学院地球化学专业,获硕士学位,2012年6月毕业于厦门大学海洋与地球学院海岸带综合管理专业,获博士学位,研究员。E-mail:yutao@tio.org.cn
基金资助:
厦门海洋研究开发院共建项目(K151301);国家海洋公益性行业科研专项(201505005)。

The protective effect of two kinds of marine polysaccharides onthe ⁶⁰Co radiation-induced damage to mice

YU Tao¹, WANG Lei¹, JI Jianda¹, YANG Qingchuan¹, ZHANG Fei¹, XU Chen², XU Jianzhong²

1. Third Institute of Oceanography, Ministry of Natural Resources, Laboratory of Marine Isotopic Technologyand Environmental Risk Assessment, Fujian Xiamen 361005;
2. Third Institute of Oceanography, Ministry ofNatural Resources, Key Laboratory of Marine Biogenetic Resources, Fujian Xiamen 361005

Received:2018-11-22 Online:2019-05-15 Published:2019-10-29

摘要/Abstract

摘要： 本研究以雄性昆明小鼠为对象,探讨低聚壳聚糖和岩藻低聚糖对其辐射损伤的保护作用。将小鼠随机分为空白对照组、辐照对照组、低聚壳聚糖低剂量组[每天0.9 mg/kg(体重)]、低聚壳聚糖高剂量组[3 mg/kg(体重)]、岩藻低聚糖低剂量组[0.9 mg/kg(体重)]和岩藻低聚糖高剂量组[3 mg/kg(体重)]6个组,采用灌胃方式,连续灌胃25天。空白对照组和辐照对照组则给予等量去离子水。在第17天除空白对照组外,各组小鼠均接受一次性全身4 Gy的⁶⁰Co γ射线照射。照射后第1天和第7天,对小鼠的免疫、遗传和抗氧化三方面指标进行检测。结果显示,灌胃低聚壳聚糖和岩藻低聚糖的小鼠的胸腺指数和脾指数均显著升高,未表现明显的剂量-效应关系,说明两种多糖可能对辐射损伤小鼠的器官损伤有一定的保护作用;灌胃多糖的小鼠股骨骨髓DNA含量显著增加,低聚壳聚糖高剂量组表现更突出,表明两种多糖能够减轻辐射诱导的遗传损伤;灌胃多糖的小鼠肝脏、心脏和肾脏的谷胱甘肽(GSH)含量显著升高,丙二醛(MDA)含量显著降低,表明两种多糖具有显著的抗氧化作用。本研究的结果表明低聚壳聚糖和岩藻低聚糖对辐射小鼠具有一定的保护作用,其作为保健品开发可能性还需进一步探讨。

关键词: 低聚壳聚糖, 岩藻低聚糖, 昆明小鼠, 辐射损伤, 保护作用

Abstract: With the rapid development and application of nuclear technology in China, the treatment to the potential danger of nuclear radiation has become a hot topic in the fields of medicine, health and society. In this study, we tried to investigate the protective effect of oligomeric chitosan and fucoidan on radiation-induced damage in the male Kunming mice. The mice were randomly divided into 6 groups: normal control group, irradiation control group, oligomeric chitosan low dose group (0.9 mg kg^-1 BW d^-1), oligomeric chitosan high dose group (3 mg kg^-1 BW d^-1), fucoidan low dose group (0.9 mg kg^-1 BW d^-1) and fucoidan high dose group (3 mg kg^-1 BW d^-1). They were given polysaccharides by gavage for 25 days except the mice in normal control group and irradiation control group were given deionized water in the same way. On the 17^th day, the mice in all groups except normal control group were irradiated with 4 Gy ⁶⁰Co γ ray. Immune index, genetic index and antioxidant activity were detected at the 1^th and 7^th day after radiation. The results showed that the thymus index and the spleen index of mice given oligomeric chitosan and fucoidan increased significantly, but no obvious dose-effect relationship was observed. It suggested that the two polysaccharides might have a protective effect on organ damage in irradiated mice. The amount of bone marrow DNA in the femur increased significantly in the groups of polysaccharide, especially the group of high dosage of oligomeric chitosan, indicating that the two kinds of polysaccharides can reduce the genetic damage induced by radiation. The content of GSH increased significantly, and the MDA content reduced significantly of the heart, liver, and kidneys in the groups of polysaccharide, which indicated that the two kinds of polysaccharides had significant antioxidant effects. The results suggested that oligomeric chitosan and fucoidan had protective effects on damage in γ ray irradiated mice. The possibility for the development of health care products in the future needs to be further explored.

Key words: oligomeric chitosan, fucoidan, Kunming mice, radiation damage, protection

中图分类号:

R142+.4

于涛, 王蕾, 纪建达, 杨青川, 张飞, 许晨, 许建中. 两种海洋生物多糖对⁶⁰Co辐射损伤小鼠保护作用的研究[J]. 辐射防护, 2019, 39(3): 242-248.

YU Tao, WANG Lei, JI Jianda, YANG Qingchuan, ZHANG Fei, XU Chen, XU Jianzhong. The protective effect of two kinds of marine polysaccharides onthe ⁶⁰Co radiation-induced damage to mice[J]. RADIATION PROTECTION, 2019, 39(3): 242-248.

参考文献

[1] 赵斌, 张军帅, 刘培勋. 辐射防护剂研究现状及其进展[J]. 核化学与放射化学, 2012, 34(1):8-13.
[2] 楚建杰, 鹿成韬, 王艳华, 等. 多糖对电离辐射防护与修复作用的研究进展[J]. 中国药师, 2015, (6):1 001-1 004.
[3] 黄震, 迟秀文, 束振华, 等. 海藻多糖对衰老小鼠的免疫调节作用[J]. 国际检验医学杂志, 2015, (13):1 801-1 803.
[4] Schepetkin I A, Faulkner C L, Nelson-Overton L K, et al. Macrophage immunomodulatory activity of polysaccharides isolated from Juniperus scopolorum[J]. International Immunopharmacology, 2005, 5(13-14):1 783-1 799.
[5] Oh J Y, Fernando I P S, Jeon Y J. Potential applications of radioprotective phytochemicals from marine algae[J]. Algea, 2016, 31(4):403-414.
[6] Kokabi M, Yousefzadi, Morteza, et al. Antioxidant activity of extracts of selected algae from the Persian Gulf, Iran[J]. Journal of Environmental Chemistry, 2013, 4(3):45-50.
[7] Kaliswari G, Mahendran S, Subalakshmi P, et al. Purification, characterization and antioxidant activity of green seaweed codium sp[J]. Advances in Pharmacology and Pharmacy, 2016, 4(2):16-21.
[8] Mohammed A, Jinkyung C, Min-Sup L, et al. Hypopigmenting effects of brown algae-derived phytochemicals: a review on molecular mechanisms[J]. Marine Drugs, 2017, 15(10):297.
[9] FENG T, SANG M, ZHUANG H, et al. In vitro and in vivo antioxidative and radioprotective capacities of polysaccharide isolated from Mesona Blumes gum[J]. Starch‐Stärke, 2017, 69(11-12): e201700056.
[10] LIU F, WANG Z, LIU J, et al. Radioprotective effect of orally administered beta-D-glucan derived from Saccharomyces cerevisiae[J]. International Journal of Biological Macromolecules, 2018, 115: 572-579.
[11] 高旭, 李丽芬, 刘斌钰. 黄芪多糖对小鼠应激能力和自由基代谢的影响[J]. 中国预防医学杂志, 2010, (2):120-121.
[12] 王俊婷. 姬松茸多糖对辐射损伤大鼠的免疫调节及抗氧化作用研究[D]. 青岛大学, 2015.
[13] 张华. 黑木耳中性多糖片段硫酸酯对辐射诱导氧化应激防护作用[D]. 哈尔滨工业大学, 2013.
[14] 杨铁虹, 贾敏, 梅其炳. 当归多糖组分促进淋巴细胞增殖及对IL-2和IFN-γ的诱生作用[J]. 中药材, 2005, 28(5):405-407.
[15] 武剑. 柴胡多糖对巨噬细胞免疫功能的调节及对TLR4信号通路的影响[D]. 复旦大学, 2012.
[16] 萨仁娜. 海带岩藻聚糖分级纯化及对肉仔鸡巨噬细胞免疫调节的研究[D]. 中国农业科学院, 2007.
[17] 王庆蓉, 马丽, 雷呈祥. 多糖抗辐射作用的研究进展[J]. 海军医学杂志, 2009, 30(3):273-276.
[18] 舒尊鹏, 杨燕妮, 王毅, 等. 枳壳多糖对辐射损伤小鼠的防护作用研究[J]. 中国药理学通报, 2018, 34(5):85-89.
[19] 张桂英, 王宝贵, 赵林伊, 等. 壳糖胺对小鼠脾、胸腺、白细胞辐射防护作用研究[J]. 长春中医药大学学报, 2000, (4):47-48.
[20] 李娜, 沈先荣, 宗杰, 等. 星虫多糖对亚急性辐射损伤小鼠造血系统的保护作用研究[J]. 中国海洋药物, 2017,(6): 53-59.
[21] 詹林盛, 张新生, 吴晓红, 等. 海带多糖的免疫调节作用[J]. 中国生化药物杂志, 2001, 22(3):116-118.
[21] 段慧全, 高子翔, 代培斌, 等. Fucoidan对巨噬细胞RAW264.7的免疫调节作用[J]. 中国微生态学杂志, 2017,(11): 1 249-1 252.
[23] 姜建华, 张学进. 壳聚糖对雄性小鼠骨髓细胞染色体的辐射防护作用[J]. 肿瘤学杂志, 2005, 11,(5):371-372.
[24] Park E, Song M J, Ahn G, et al. Radioprotective effect of fucoidan against hematopoietic and small intestinal stem cells of γ-ray irradiated mice[J]. Korean Journal of Veterinary Research, 2008, 48(4): 393-399.
[25] 郭剑平, 杨仲田, 张华屏, 等. 低聚壳聚糖对⁶⁰Co辐射损伤小鼠骨髓的防护作用研究[J]. 中华放射医学与防护杂志, 2010, 30(4):430-431.
[26] 郭剑平, 杨仲田, 周朝东. 低聚壳聚糖对小鼠造血系统的辐射损伤防护作用研究[J]. 中国辐射卫生, 2009, 18(3):260-261.
[27] 张宸阁, 卢卫红. 孔石莼多糖对辐射诱发小鼠骨髓微核抑制作用研究[J]. 食品科技, 2011, (12):196-198.
[28] 季宇彬, 高世勇, 张秀娟. 羊栖菜多糖诱导肿瘤细胞凋亡的研究[J]. 中国中药杂志, 2004, 29(3):245.
[29] 孟庆勇, 刘志辉, 郑辉, 等. 半叶马尾藻多糖对γ射线氧化损伤小鼠的保护作用[J]. 中国海洋大学学报自然科学版, 2004, 34(4):589-593.
[30] 李解, 吴家乐, 谭晓琴, 等. 雅安藏茶茶多糖对⁶⁰Co-γ射线辐照损伤小鼠抗氧化和造血功能的防护作用[J]. 核农学报, 2017(8): 1 509-1 514.
[31] 陶传云, 周革非, 马伟伟. 海黍子褐藻糖胶的分离及抗氧化与保湿活性[J]. 食品工业, 2018,(4): 225-229.

两种海洋生物多糖对⁶⁰Co辐射损伤小鼠保护作用的研究

The protective effect of two kinds of marine polysaccharides onthe ⁶⁰Co radiation-induced damage to mice

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 1

Metrics

本文评价

推荐阅读 0