黄芪甲苷对肝细胞放射损伤预防作用的机制研究

辐射防护 ›› 2017, Vol. 37 ›› Issue (4): 309-316.

黄芪甲苷对肝细胞放射损伤预防作用的机制研究

胡雅梦^1,2, 龙颖¹, 陈科良¹, 刘奔波¹, 何淑雅¹, 黄波^1,3

1.南华大学公共卫生学院,湖南衡阳 421001;
2.郴州市疾病预防控制中心,湖南郴州 423000;
3.北京放射与辐射医学研究所放射生物学北京市重点实验室,北京 100850

收稿日期:2016-09-06 出版日期:2017-07-20 发布日期:2021-11-11
通讯作者: 黄波。E-mail:huangbo0930@163.com
作者简介:胡雅梦(1989—),女,2016年毕业于南华大学公共卫生与预防医学专业,获硕士学位,主治医师。E-mail:86066530@qq.com
基金资助:
国家自然科学基金项目(No. 81272994);湖南省自然科学基金项目(2016JJ2115);湖南省卫生计生委科研计划课题项目(C2015-17);南华大学博士启动基金(2015XQD31);放射生物学北京市重点实验室开放课题(BKLR2016-01)。

Mechanism of radioprotection effects of Astragaloside IV on the liver cell

Hu Yameng^1,2, Long Ying¹, Chen Kelian¹, Liu Bengbo¹, He Shuya¹, Huang Bo^1,3

1. School of Public Health University of South China, Hunan Hengyang 421001;
2. Chenzhou Center for Disease Control and Prevention, Hunan Chenzhou 423000;
3. Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Beijing 100850

Received:2016-09-06 Online:2017-07-20 Published:2021-11-11

摘要/Abstract

摘要： 本文研究了黄芪甲苷(Astragaloside IV,ASIV)对肝细胞放射损伤防护的机制。以γ射线照射L-02细胞建立辐射损伤模型,分为对照组、黄芪甲苷组(80 μg/mL)、照射组(4 Gy)、黄芪甲苷+照射组(80 μg/mL +4 Gy),于照射前12 h更换含有黄芪甲苷浓度为80 μg/mL的培养基。采用流式细胞术检测黄芪甲苷对照后L-02细胞早期凋亡率及细胞周期的影响;以罗丹明123荧光探针检测黄芪甲苷对照后L-02细胞线粒体膜电位改变的影响;免疫荧光法观察γH2AX焦点数;通过Western Blot检测细胞中Nrf2、HO-1、NQO1的蛋白表达变化。结果显示:照后细胞凋亡率随着药物浓度的增加而降低,黄芪甲苷能有效的缓解电离辐射引起的细胞G₂期阻滞;黄芪甲苷+照射组细胞线粒体膜电位均高于照射组;黄芪甲苷+照射组γH2AX焦点数明显少于照射组;照射组细胞内Nrf2、HO-1及NQO1表达升高,黄芪甲苷+照射组Nrf2、HO-1及NQO1表达则是随着药物浓度增加而降低。综上可知,黄芪甲苷对L-02细胞具有辐射损伤预防作用,其机制可能是通过Nrf2途径来减轻电离辐射所致的损伤。

关键词: 黄芪甲苷, 凋亡, 细胞周期, γH2AX, Nrf2

Abstract: The radioprotection effects and molecular mechanism of Astragaloside IV (ASIV) on the liver cell L-02 was studied. The experiments were conducted with four groups: the control group, the ASIV group, the irradiation group and the ASIV+irradiation group. The rate of apoptosis and cell cycle were detected by flow cytometry and Rhodamine-123 fluorescent probe was used to detect potential alternation of the mitochondrial membrane in L-02 cell. γH2AX foci were detected by immunofluorescence confocal microscopy while the expressions of Nrf2, HO-2, NQO1 proteins were observed by western blot method. The results showed that the apoptosis rate and the arrested cell cycle G2 decreased with increasing concentrations of Astragaloside IV after irradiation and the membrane potential increased with the rise of Astragaloside IV concentration as observed by spectrophotofluorimetry after Rh123 dyeing. The confocal microscopy results showed that the number of γH2AX focus in Astragaloside+irradiation group was significantly less than in the irradiation group. Western Blot results indicated that the expression of Nrf2, HO 1-and NQO1 increased in the irradiated cells, while the opposite trend showed in Astragaloside + irradiation group with increasing drug concentration.

Key words: Astragaloside IV, apoptosis, cell cycle, γH2AX, Nrf2

中图分类号:

Q691

胡雅梦, 龙颖, 陈科良, 刘奔波, 何淑雅, 黄波. 黄芪甲苷对肝细胞放射损伤预防作用的机制研究[J]. 辐射防护, 2017, 37(4): 309-316.

Hu Yameng, Long Ying, Chen Kelian, Liu Bengbo, He Shuya, Huang Bo. Mechanism of radioprotection effects of Astragaloside IV on the liver cell[J]. RADIATION PROTECTION, 2017, 37(4): 309-316.

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