低剂量γ辐射对青海弧菌Q67的毒性研究

辐射防护 ›› 2017, Vol. 37 ›› Issue (2): 138-144.

低剂量γ辐射对青海弧菌Q67的毒性研究

赵维超^1,3, 胡南¹, 丁德馨¹, 龙鼎新², 何淑雅², 李乐², 胡淼¹, 冯永富¹

1.南华大学铀矿冶生物技术国防重点学科实验室,湖南衡阳 421001;
2.南华大学公共卫生学院,湖南衡阳 421001;
3.南华大学环境保护与安全工程学院,湖南衡阳 421001

收稿日期:2016-03-14 出版日期:2017-03-20 发布日期:2021-11-11
作者简介:赵维超(1981—),女,2004年毕业于吉首大学,2015年毕业于南华大学公共卫生学院卫生毒理学专业,获硕士学位,现为南华大学环安学院安全工程专业博士研究生。E-mail:59579883@qq.com
基金资助:
国防基础科研重点项目(B3720132001);国家自然科学基金项目(81272993)。

Study on toxicity of low dose γ radiation to Qinghai Vibrio Q67

Zhao Weichao¹, Hu Nan¹, Ding Dexin¹, Long Dingxin², He Shuya², Li Le², Hu Miao¹, Feng Yongfu¹

1. Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hunan Hengyang 421001;
2. School of Public Health, University of South China, Hunan Hengyang 421001

Received:2016-03-14 Online:2017-03-20 Published:2021-11-11

摘要/Abstract

摘要： 分别采用生物辐照仪和模拟铀矿室作为γ辐照源,研究了低剂量和超低剂量γ照射对青海弧菌Q67发光强度和光密度(OD₆₀₀)的影响。实验结果表明,青海弧菌Q67在5.5 cGy/min照射剂量率、不同累积照射剂量γ射线照射下,在一定累积照射剂量范围内,发光强度的抑制率无显著差异,当累积照射剂量超过该范围的累积照射剂量的阈值后,发光强度抑制率与累积照射剂量呈正相关。青海弧菌Q67在320 mGy累积照射剂量,5.5、14和51 cGy/min 3个不同照射剂量率γ射线照射下,发光强度在低照射剂量率、长时间的γ射线照射条件下抑制率更大。青海弧菌Q67在长时间超低γ射线照射剂量率的条件下,菌液的OD₆₀₀值的抑制率在24 h达到最大值,此后逐渐降低,其中30和120 μGy/h两组剂量率照射下,青海弧菌Q67菌体数呈现低剂量兴奋效应。通过测定低剂量和超低剂量γ射线照射对青海弧菌Q67的发光强度和菌液OD₆₀₀值的抑制率,可反映一定剂量范围内的γ射线照射对青海孤菌Q67的综合毒性作用。青海孤菌Q67对γ射线具有较好的敏感性,可用作低剂量γ射线毒性评价的生物材料。

关键词: 青海弧菌Q67, 低剂量率照射, 模拟铀矿室照射, 生物预警

Abstract: The effects of low dose and ultra-low dose γ radiation on luminous intensity and optical density (OD₆₀₀) of Qinghai Vibrio Q67 were studied using biological irradiator and simulated uranium irradiation chamber as γ radiation source. The results showed that the luminous intensity inhibition rates of Qinghai Vibrio Q67 were no significant difference when the cumulative radiation dose varied within a certain range at a dose rate of 5.5 cGy/min. Once over the dose threshold value in this range, there was a positive correlation between the luminous intensity inhibition rate and the cumulative radiation dose. Moreover, Qinhai Vibrio Q67 irradiating with 320 mGy cumulative radiation dose at three different irradiated dose of 5.5, 14 and 51 cGy/min, it was found that its luminous intensity inhibition rate increased on the condition of low dose rate and long time γ ray radiation. Qinhai Vibrio Q67 irradiated with γ rays at ultra low dose rate for long time the inhibition rate of the OD₆₀₀ value reached the maximum at 24 h, and then reduced gradually. The hormesis of Qinghai Vibrio Q67 occurred with γ dose rates both at 30 and at 120 μGy/h. It was feasible to establish biological early warning method by calculating the inhibition rate of luminous intensity and OD₆₀₀ values of Qinghai Vibrio Q67 in low dose and ultra-low dose γ irradiation.

Key words: Qinghai Vibrio Q67, low dose rate exposure, simulated uranium irradiation chamber, biological warning

中图分类号:

X826

赵维超, 胡南, 丁德馨, 龙鼎新, 何淑雅, 李乐, 胡淼, 冯永富. 低剂量γ辐射对青海弧菌Q67的毒性研究[J]. 辐射防护, 2017, 37(2): 138-144.

Zhao Weichao, Hu Nan, Ding Dexin, Long Dingxin, He Shuya, Li Le, Hu Miao, Feng Yongfu. Study on toxicity of low dose γ radiation to Qinghai Vibrio Q67[J]. RADIATION PROTECTION, 2017, 37(2): 138-144.

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