HPRT基因用于γ射线和苯并芘鉴别诊断的初步研究

辐射防护 ›› 2016, Vol. 36 ›› Issue (1): 26-30.

HPRT基因用于γ射线和苯并芘鉴别诊断的初步研究

刘建功, 张忠新, 党旭红, 刘红艳, 王超, 段志凯

中国辐射防护研究院,太原 030006

收稿日期:2015-05-22 出版日期:2016-01-20 发布日期:2021-11-12
通讯作者: 段志凯。E-mail:duanzhikai@cirp.org.cn
作者简介:刘建功(1981—),男,2005毕业于山西医科大学临床医学专业,2008年毕业于山西医科大学生物化学与分子生物学专业,获硕士学位,助理研究员。E-mail:liujiangong2005@126.com

A primary study on deletion of HPRT in differentiated diagnosis between γ ray and benzo-pyrene

Liu Jiangong, Zhang Zhongxin, Dang Xuhong, Liu Hongyan, Wang Chao, Duan Zhikai

China Institute for Radiation Protection, Taiyuan 030006

Received:2015-05-22 Online:2016-01-20 Published:2021-11-12

摘要/Abstract

摘要： 为了解γ射线和苯并芘对细胞次黄嘌呤鸟嘌呤磷酸核糖基转移酶(hypoxanthine guanine phosphoribosyl transferase,HPRT)基因突变的影响,探索一种用于鉴别诊断γ射线和苯并芘引起的细胞损伤的早期检测敏感指标,采集了3名健康人血液,每人16 mL,分为16组,其中,10个组分别进行⁶⁰Co γ射线照射(0～5 Gy),另外6个组分别进行苯并芘染毒(0～10 μg/mL),使用荧光定量聚合酶链式反应(quantitative polymerase chain reaction,qPCR)方法分析γ射线和苯并芘对细胞HPRT基因突变位点频率的变化情况,从而筛选γ射线和苯并芘对细胞损伤的HPRT差异突变位点。结果表明,0～5 Gy剂量范围内,HPRT的外显子2和外显子5突变频率随照射剂量增加而上调,通过拟合建立了突变频率与照射剂量间的剂量-效应直线方程,外显子2:y=0.360 3+0.110 68x,R²=0.99,p<0.01,外显子5:y=0.429 8+0.082 3x,R²=0.93,p<0.01;而0～10 μg/mL苯并芘染毒后,HPRT基因的外显子2和外显子5突变频率随染毒浓度增加没有发生明显改变。γ射线所引起的外显子5突变频率与苯并芘相比有显著性差异(p<0.05),HPRT基因外显子5有望成为一种γ射线和苯并芘的鉴别点。

关键词: γ射线, 苯并芘, 突变频率, 荧光定量PCR, HPRT

Abstract: To investigate the changes of deletion of HPRT exposure to γ ray and benzo-pyrene, and to find a differential diagnosis index for cell injury induced by γ ray and benzo-pyrene human peripheral blood samples from three persons were exposed to ⁶⁰Co γ rays at 10 different doses (0—5 Gy) and benzo-pyrene at 6 different concentrations (0—10 μg/mL), its DNA was isolated from white cell. The changes of deletion of HPRT exposure to γ ray and benzo-pyrene were detected by real time quantitative PCR. The relationship between deletion frequency of HPRT exon and irradiation dose represents certain linear correlation from 0—5 Gy. A linear regression equation has been obtained using statistics regression analysis software: exon-2,y=0.360 3+0.110 68x,R²=0.99,p<0.01;exon-5,y=0.429 8+0.082 3x,R²=0.93,p<0.01 while, the relationship between deletion frequency of HPRT and different concentrations (0—10 μg/mL) represents no statistical significance. There is an obvious influence on deletion frequency of exon 5 of HPRT induced by γ ray,while there is no obvious influence on deletion frequency of exon 5 of HPRT induced by benzo-pyrene,which may be a new differential diagnosis index in the future.

Key words: γ ray, benzo-pyrene, deletion frequency, real time quantitative PCR, HPRT

中图分类号:

R144.1

刘建功, 张忠新, 党旭红, 刘红艳, 王超, 段志凯. HPRT基因用于γ射线和苯并芘鉴别诊断的初步研究[J]. 辐射防护, 2016, 36(1): 26-30.

Liu Jiangong, Zhang Zhongxin, Dang Xuhong, Liu Hongyan, Wang Chao, Duan Zhikai. A primary study on deletion of HPRT in differentiated diagnosis between γ ray and benzo-pyrene[J]. RADIATION PROTECTION, 2016, 36(1): 26-30.

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