137Cs γ 射线累积照射SD大鼠后肝脏蛋白质组的分析

摘要/Abstract

摘要： 采用相对和绝对定量的同位素标签技术(Isobaric tags for relative and absolute quantitation,iTRAQ)分析累积受¹³⁷Cs γ射线照射大鼠肝脏组织的蛋白质表达,筛选出差异蛋白质,对差异表达的蛋白质进行GO注释分析及相互作用网络分析,探讨¹³⁷Cs γ 射线累积照射对SD大鼠肝脏蛋白质的影响。结果显示,质谱数据经蛋白质数据库搜索,在3组样品中有定量信息的蛋白质有2 220个;0.2 Gy照射组与对照组相比,差异蛋白有149种,52种蛋白上调,97种蛋白下调;2 Gy照射组有243种差异蛋白,123种上调,120种下调;两组共同表达差异的蛋白有122种,其中,52种蛋白表达上调,70种蛋白表达下调;利用GO注释对122个共同差异蛋白质功能进行聚类分析,结果表明:根据分子功能注释,差异蛋白以酶催化、蛋白质结合、核酸结合等功能为主,另外少数蛋白还有翻译调控活性;根据细胞组成注释,多数蛋白参与细胞胞浆、细胞核、细胞膜的组成;根据生物学过程注释,多数蛋白参与代谢调节和生物过程调节。利用PAJEK软件对两个剂量组共同差异表达的蛋白进行相互作用网络分析。结果显示SF3B1、ATP6V1C1是蛋白相互作用网络的重要节点,这两个蛋白直接或间接地与其它差异蛋白存在相互作用。上述研究结果表明,¹³⁷Cs γ射线累积照射可诱导SD大鼠肝脏组织蛋白质组的改变,SF3B1、ATP6V1C1蛋白可能在累积照射的肝脏损伤机制中发挥作用。

关键词: 累积照射, 蛋白质组, 同位素标记的相对和绝对定量技术, 相互作用网络

Abstract: This study was designed to investigate the changes of proteomics in liver of Sprague-Dawley(SD)rats after accumulated irradiation by ¹³⁷Cs γ ray. Isobaric tags for relative and absolute quantitation (iTRAQ) was used to analyze the different protein expression in liver of irradiated rats. Thirty mature SD rats were randomly divided into three groups: 0.2 Gy group, 2 Gy group and healthy control group. Rats were irradiated at dose rate of 0.336 mGy/min for 10 days and 20 days continuously. Gene Ontology and protein-protein interaction network analysis were conducted using softwares. The results demonstrated that 2 220 protein spots were identified. 122 proteins were differentially expressed both in two groups compared with control, of which 52 were up-regulated and 70 down-regulated. Based on the information of GO categories,these differentially expressed proteins mainly located in the cytoplasm and membrane,involving in the function of protein binding and catalytic activity. Analysis with the PAJEK software demonstrated that those 16 differentially expressed proteins could form a complicated interaction network where SF3B1, ATP6V1C1 might be key nodes.

Key words: accumulated irradiatin, proteomics, isobaric tags for relative and absolute quantitation (iTRAQ), interaction network

中图分类号:

Q691

左雅慧, 党旭红, 原雅艺, 王超, 张忠新, 刘建功, 张睿凤, 段志凯. ¹³⁷Cs γ 射线累积照射SD大鼠后肝脏蛋白质组的分析[J]. 辐射防护, 2015, 35(2): 87-92.

Zuo Yahui, Dang Xuhong, Yuan Yayi, Wang Chao, Zhang Zhongxin, Liu Jiangong, Zhang Ruifeng, Duan Zhikai. Study on liver proteomics of rats accumulated ¹³⁷Cs γ ray irradiation[J]. RADIATION PROTECTION, 2015, 35(2): 87-92.

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¹³⁷Cs γ 射线累积照射SD大鼠后肝脏蛋白质组的分析

Study on liver proteomics of rats accumulated ¹³⁷Cs γ ray irradiation

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