微生物矿化法固结模拟放射性核素锶的研究

辐射防护 ›› 2015, Vol. 35 ›› Issue (2): 65-70.

微生物矿化法固结模拟放射性核素锶的研究

肖湘竹¹, 竹文坤², 张友魁², 牟涛¹, 段涛², 聂小琴³, 罗学刚²

1.中国工程物理研究院,四川绵阳 621000;
2.“核废物与环境安全”国防重点学科实验室, 四川绵阳 621000;
3.四川大学原子核科学技术研究所,成都 610065

收稿日期:2014-04-16 出版日期:2015-03-20 发布日期:2024-10-21
通讯作者: 竹文坤。E-mail: zhuwk0@163.com
作者简介:肖湘竹(1972—),女,1993年7月毕业于西南大学化学专业,2004年12月毕业于西南大学环境工程专业,获硕士学位,副研究员。E-mail: xiao_xz839@126.com
基金资助:
国家国防科技工业局项目(科工二司[2011]1081号);国家自然科学基金资助项目(11075134);核废物与环境安全国防重点学科实验室开放基金资助(12zxnp08);中国工程物理研究院培训中心基金项目(14PX01)。

Study on simulated radionuclide strontium mineralized by bacteria

Xiao Xiangzhu¹, Zhu Wenkun², Zhang Youkui², Mu Tao¹, Duan Tao², Nie Xiaoqin³, Luo Xuegang²

1. China Academy of Engineering Physics,Sichuan Mianyang 621000;
2. Fundamental Science on Nuclear Waste and Environmental Security Laboratory, Sichuan Mianyang 621000;
3. Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610065

Received:2014-04-16 Online:2015-03-20 Published:2024-10-21

摘要/Abstract

摘要： 采用微生物矿化法将游离态Sr²⁺固结为稳定的不可溶的碳酸盐形态,为放射性核素锶的处理提供参考。选取碳酸盐矿化菌进行培养,利用其酶化作用分解尿素,产生CO₃^2-,与溶液中Sr²⁺结合生成不可溶性碳酸盐。通过微生物矿化试验,分析了pH值、温度、接种量、培养时间、Sr²⁺及尿素浓度等因素对Sr²⁺固结率的影响,并采用EDS、XRD、SEM、FT-IR等检测手段对矿化产物的物相和形貌等进行了分析。结果表明:矿化产物为大小不均近球形SrCO₃晶体,相互团聚,且细菌在沉淀生成过程中起到了重要作用;当Sr²⁺浓度为0.02 mol/L、尿素为20 g/L、接种量体积分数为6.0%、pH值为8.0时,在30 ℃培养48 h时,微生物矿化对Sr²⁺固结率可达98.5%左右。

关键词: 微生物, 矿化, 碳酸盐, 放射性核素锶

Abstract: Sr²⁺ was transformed into insoluble state to provide a reference method for dealing with radionuclide strontium. Bacillus pasteurii was cultivated with medium containing urea. Enzyme of urease was produced and urea was discomposed to CO₃^2-, which could put soluble Sr²⁺ into insoluble carbonate. The particle size and morphology of the solidifying product was characterized by energy dispersive spectroscopy (EDS), X-ray diffraction (XRD) and scanning electronic microscope (SEM). Factors such as pH, temperature, inoculum concentration, incubation time, concentration of Sr²⁺and urea on solidifying rate of Sr²⁺was studied. It showed that the solidifying product was composed of aggregative globular SrCO₃crystals in unequal size. The bacillus played an important role in the precipitating process. Cultivating 48 h at 30 ℃, the solidifying rate reached a peak as about 98.5% when the concentration of Sr²⁺and urea, inoculum concentration and pH was 0.02 mol/L, 20 g/L, 6.0% and pH 8.0 respectively.

Key words: microorganism, mineralization, carbonate, radionuclide strontium

中图分类号:

TL941+.19

肖湘竹, 竹文坤, 张友魁, 牟涛, 段涛, 聂小琴, 罗学刚. 微生物矿化法固结模拟放射性核素锶的研究[J]. 辐射防护, 2015, 35(2): 65-70.

Xiao Xiangzhu, Zhu Wenkun, Zhang Youkui, Mu Tao, Duan Tao, Nie Xiaoqin, Luo Xuegang. Study on simulated radionuclide strontium mineralized by bacteria[J]. RADIATION PROTECTION, 2015, 35(2): 65-70.

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