辐射防护 ›› 2021, Vol. 41 ›› Issue (4): 315-320.

• 辐射生物效应 • 上一篇    下一篇

低剂量辐射对小鼠肠道损伤的影响

胡昌坤1,2, 张雪梅2,3, 马增春2, 高月2   

  1. 1.安徽医科大学,合肥 230032;
    2.军事科学院军事医学研究院辐射医学研究所,北京 100850;
    3.广东药科大学,广州 510006
  • 收稿日期:2020-12-24 出版日期:2021-07-20 发布日期:2021-09-23
  • 通讯作者: 高月。E-mail:gaoyue@bmi.ac.cn
  • 作者简介:胡昌坤(1995—),男,2018年毕业于安徽医科大学临床医学院药学专业,现为安徽医科大学基础医学院药学专业在读硕士研究生。E-mail:changkunhu@126.com
  • 基金资助:
    国家自然科学基金项目(81873063)。

Effects of low dose radiation on intestinal injury in mice

HU Changkun1,2, ZHANG Xuemei2,3, MA Zenchun2, GAO Yue2   

  1. 1. Anhui Medical University,Hefei 23003;
    2. Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of Military Sciences,Beijing 100850;
    3. Guangdong Pharmaceutical University, Guangzhou 510006
  • Received:2020-12-24 Online:2021-07-20 Published:2021-09-23

摘要: 采用3种不同低剂量(L组0.04 Gy/d、M组0.12 Gy/d、H组0.2 Gy/d)辐射对3组Balb/c小鼠进行连续5天的照射(分别累积照射0.2、0.6、1.0 Gy),设立对照组(NC),照射结束后测定小鼠体重、外周血象、脏器指数、小肠组织中丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、免疫细胞因子(IL-1β、IL-2、IL-6、TNF-α)、DNA损伤、细胞凋亡等指标的变化。通过比较不同低剂量辐射下小鼠肠道损伤指标的变化,以探讨低剂量辐射小鼠肠道损伤最佳照射剂量,为低剂量辐射小鼠肠道损伤模型的建立提供科学依据。结果表明,连续照射5天后,各照射组相比于对照组,小鼠体重、脏器指数均有不同程度下降(脾脏指数L组p<0.05、M、H组p<0.01);小肠组织中MDA含量明显升高(L组p<0.05、M、H组p<0.01);SOD含量有不同程度下降;TNF-α、IL-2、IL-1β和IL-6作为代表性促炎因子呈剂量依赖性升高(IL-1β:M、H组p<0.05,IL-2:M组p<0.05、H组p<0.01,TNF-α:M、H组p<0.05);M、H组有明显DNA损伤及细胞凋亡。通过以上结果得出本次低剂量辐射小鼠肠道损伤模型的最佳照射剂量及方法为0.12 Gy/d连续照射5天累积0.6 Gy。

关键词: 低剂量辐射, 模型, 小鼠, 肠道

Abstract: Objective: To compare the changes of intestinal injury indexes in mice exposed to different low-dose radiation, so as to provide scientific basis for the establishment of intestinal injury model in mice exposed to low-dose radiation.Methods: Balb/c mice in three groups were irradiated with three different low dose rates (0.04 Gy/d, 0.12 Gy/d, and 0.2 Gy/d) for five consecutive days (cumulative exposure of 0.2, 0.6, and 1.0 Gy, respectively), and a control group (NC) was established. After the irradiation, the body weight, peripheral blood picture, organ index, malondialdehyde (MDA) content in small intestinal tissue, superoxide dismutase (SOD) activity, immune cytokine (IL-1β, IL-2, IL-6, TNF-α), DNA damage were determined, to explore the optimal dose of low dose radiation for intestinal injury in mice.Results: After five days of continuous irradiation, compared with the control group, the body weight and organ index of mice in each irradiation group were decreased to different extents (p< 0.05 in the spleen index L group, p< 0.01 in the M and H groups). MDA content in small intestinal tissue was significantly increased (p< 0.05 in Group L, and p< 0.01 in Group M and H); SOD content was decreased to different degrees. TNF-α, IL-2, IL-1β and IL-6, the typical proinflammatory factors, showed dose-dependent increases (IL-1β: M, group H p< 0.05, IL-2: M group p< 0.05, group H p< 0.01, TNF-α: M, group H p< 0.05). Group M and H had significant DNA damage and apoptosis.Conclusion: The optimal dose and method for low dose rate radiation induced intestinal injury in mice is 0.12 Gy/d continuous irradiation and 0.6 Gy accumulation for 5 days.

Key words: low dose radiation, model, mouse, intestinal

中图分类号: 

  • R818