基于元素分析仪的生物基体碳含量分析

doi:10.27045/j.1000-8187.202603006

辐射防护 ›› 2026, Vol. 46 ›› Issue (3): 234-242.doi: 10.27045/j.1000-8187.202603006

基于元素分析仪的生物基体碳含量分析

黄慧^1,2, 原妮^1,2, 安全^1,2, 罗茂益^1,2,3, 王志鹏¹, 郭媛媛^1,2, 王远飞¹

1.中国辐射防护研究院,太原 030006;
2.核药研发转化与精准防护山西省重点实验室,太原 030006;
3.苏州大学放射医学协同创新中心,江苏苏州 215006

收稿日期:2025-09-24 出版日期:2026-05-20 发布日期:2026-06-18
通讯作者: 罗茂益。E-mail:luomaoyi@cirp.org.cn
作者简介:黄慧(2001—),女,2023年毕业于大连理工大学环境工程专业,获学士学位,现为中国辐射防护研究院环境科学专业在读硕士研究生。E-mail:1224109236@qq.com
基金资助:
山西省重点研发计划项目(202102130501022);中国辐射防护研究院创新团队项目(YC22000702)。

Determination of carbon content in biological samples using elemental analyzer

HUANG Hui^1,2, YUAN Ni^1,2, AN Quan^1,2, LUO Maoyi^1,2,3, WANG Zhipeng¹, GUO Yuanyuan^1,2, WANG Yuanfei¹

1. China Institute for Radiation Protection,Taiyuan 030006;
2. Nuclear Drug R&D Transformation and Precision Protection Key Laboratory of Shanxi Province,Taiyuan 030006;
3. Collaborative Innovation Center of Radiology, Soochow University, Jiangsu Suzhou 215006

Received:2025-09-24 Online:2026-05-20 Published:2026-06-18

摘要/Abstract

摘要： ¹⁴C是人体物质平衡研究中常用的标记核素,可用于新药药代动力学研究。采用高灵敏加速器质谱仪测量生物样品中的¹⁴C/¹²C比值,联合元素分析仪分析样品中的碳含量,可反映¹⁴C标记物在不同生物基体(包括血清、血浆、全血、尿液)中的含量。本文通过研究元素分析仪测量生物样品中碳含量的关键参数,实现了生物样品中碳含量的高精度测量。结果表明,在优化条件下(样品取样量为60~100 mg、通氧量≥75 mL),各类生物样品中碳含量测量的相对标准偏差<5%。可用于不同类型生物样品中碳含量的高精度分析,为生物样品中¹⁴C活度准确计算提供可靠的支撑。

关键词: 碳含量, 生物样品, 元素分析仪

Abstract: ¹⁴C,a long-lived radionuclide, is widely used as a tracer in human mass balance and pharmacokinetic studies. The ¹⁴C/¹²C ratio in biological samples was measured by a high-sensitivity accelerator mass spectrometer, and the carbon content in the samples was analyzed by the elemental analyzer, which can reflect the content of ¹⁴C markers in different biological matrices ( including serum, plasma, whole blood and urine). In this paper, a high-precision measurement program for carbon content in biological samples was established by studying the key parameters of carbon content in biological samples measured by the elemental analyzer. The results show that the relative standard deviation of carbon content measurement in various biological samples is less than 5% when the sample sampling amount is 60~100 mg and the oxygen flow is ≥75 mL. It can be applied to the high-precision analysis of carbon content in different types of biological samples, and provides an effective analytical method for the accurate calculation of ¹⁴C activity in subsequent samples.

Key words: carbon content, biological samples, elemental analyzer

中图分类号:

X830.2

黄慧, 原妮, 安全, 罗茂益, 王志鹏, 郭媛媛, 王远飞. 基于元素分析仪的生物基体碳含量分析[J]. 辐射防护, 2026, 46(3): 234-242.

HUANG Hui, YUAN Ni, AN Quan, LUO Maoyi, WANG Zhipeng, GUO Yuanyuan, WANG Yuanfei. Determination of carbon content in biological samples using elemental analyzer[J]. RADIATION PROTECTION, 2026, 46(3): 234-242.

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基于元素分析仪的生物基体碳含量分析

Determination of carbon content in biological samples using elemental analyzer

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