关键词: 锶离子, 斑马鱼, 富集, 维拉帕米, 金属硫蛋白

Abstract: Radioactive strontium has a strong affinity with bone, therefore strontium transfer parameters in the body are key parameters for evaluating the environmental impact of nuclear facilities. By using Danio rerio as research object, the enrichment and excretion patterns of strontium ions in Danio rerio are explored, and the moderation effect of verapamil and metallothionein were assessed. The results show that when Danio rerio was exposed to strontium solutions of 0, 10, 50, and 250 mg/L, the enrichment rate constants and bioconcentration factors were negatively correlated with the exposure concentration of strontium ions. A two-way kinetic model was used to simulate the theoretical equilibrium state of strontium accumulation in the whole Danio rerio, bones, and liver, which was positively correlated with the exposure concentration of strontium ions and the accumulation time, and the accumulation rate decreased as accumulation time increased. Under the same strontium concentration exposure level, the accumulation amount and rate of strontium in Danio rerio and its tissues from high to low were: bones ＞ liver ＞ whole body, and the excretion rate shows that: whole body ＞ liver ＞ bones. In a strontium environment of 50 mg/L, the accumulation of strontium in the whole body, bones, and liver was significantly reduced in theapamil group, and the strontium content in the liver was significantly reduced in the metallothionein group. This indicates that verapamil has a inhibitory effect on the accumulation of strontium in Danio rerio, and metallothionein has a certain promoting effect on the excretion of strotium from the liver of Danio rerio.

Key words: strontium ions, Danio rerio, enrichment, verapamil, metallothionein