藤茶提取液对电离辐射损伤造血系统的防护作用研究

摘要/Abstract

摘要： 采用1,1-二苯基-2-苦基肼自由基(DPPH)和总抗氧化能力检测(ABTS)两种方法体外检测藤茶提取液的抗氧化能力;然后进行生存率实验,将40只雄性C57BL/6J小鼠随机分为照射组、照射+藤茶提取液低、中、高剂量组(0.8 g/kg、1.6 g/kg、2.4 g/kg),所有小鼠均进行致死剂量7.2 Gy照射,检测藤茶提取液对照射后小鼠的生存影响;随后在电离辐射损伤造血系统实验中将50只雄性C57BL/6J小鼠随机分为5组:空白对照组、照射组、照射+藤茶提取液低、中、高剂量组,经4 Gy全身照射后,观察藤茶提取液对电离辐射后小鼠脏器指数、造血指标等的影响,并测定肝脏中总超氧化物歧化酶(T-SOD)和谷胱甘肽(GSH)的变化。实验结果表明,藤茶提取液对DPPH自由基清除率为74.35%,对ABTS自由基清除率为93.14%,能够提高7.2 Gy照射下小鼠的生存率(照射组生存率为30%,低剂量组生存率为100%,中剂量组生存率为90%,高剂量组生存率为90%),并改善4 Gy全身照射所导致造血系统损伤小鼠的脏器指数和造血指标,还能升高照射小鼠肝脏中T-SOD活力以及GSH的含量。藤茶提取液具有较强的抗氧化能力,能提高电离辐射后小鼠的生存率,并改善其引起的造血系统损伤,有望成为电离辐射导致造血系统损伤的中药防护剂。

关键词: 藤茶提取液, 抗氧化能力, 造血损伤, 电离辐射防护

Abstract: To study the protective effect of Ampelopsis grossedentata extract on the function of mice hematopoietic system irradiated by ionizing radiation, two methods, DPPH and ABTS, were used to detect the antioxidant ability of Ampelopsis grossedentata extract. In the survival experiment, 40 male C57BL/6 mice were randomly divided into irradiation group, irradiation+Ampelopsis grossedentata extract low dose, medium dose and high dose groups (0.8 g/kg, 1.6 g/kg, 2.4 g/kg). All mice were irradiated with a lethal dose of 7.2 Gy to test the survival effect of Ampelopsis grossedentata extract on mice. In the experiment of radiation damage to hematopoietic system, 50 male C57BL/6 mice were randomly divided into 5 groups: blank control group, irradiation group, irradiation+ Ampelopsis grossedentata extract low dose, medium dose and high dose groups. After 4 Gy total body irradiation, the effects of Ampelopsis grossedentata extract on organ indices and hematopoietic indexes of irradiated mice were observed, and the changes of T-SOD and GSH in the liver were measured. The scavenging rate of DPPH radicals by Ampelopsis grossedentata extract was 74.35% and that of ABTS radicals was 93.14%. Ampelopsis grossedentata extract improved the survival rate of mice irradiated with 7.2 Gy (30% in the irradiated group, 100% in the low dose group, 90% in the medium dose group, and 90% in the high dose group), and improved the organ index and hematopoietic index of mice with hematopoietic system damage caused by 4 Gy irradiation. Ampelopsis grossedentata extract has antioxidant ability, which can improve the survival rate of irradiated mice and can also improve the hematopoietic system damage induced by radiation, and has certain protective effects.

Key words: Ampelopsis grossedentata extract, anti-oxidation ability, hematopoietic damage, protection against ionizing radiation

中图分类号:

R818

王志允, 勾文峰, 郭江红, 许飞飞, 李祎亮, 侯文彬. 藤茶提取液对电离辐射损伤造血系统的防护作用研究[J]. 辐射防护, 2024, 44(2): 182-191.

WANG Zhiyun, GOU Wenfeng, GUO Jianghong, XU Feifei, LI Yiliang, HOU Wenbin. Study on the protective effect of Ampelopsis grossedentata extract on the hematopoietic system damage caused by ionizing radiation[J]. RADIATION PROTECTION, 2024, 44(2): 182-191.

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