Abstract: To explore the effect of ionizing radiation on CDKN1A gene expression in human peripheral blood, blood samples were exposed to ⁶⁰Co γ-rays at different doses. The resulted CDKN1A mRNA expression change was observed by real time PCR. For a dose of 0～2 Gy, the relationship between the CDKN1A-dose represents certain line correlation. A linear regression equation(Y=0.8465+1.1015D,R=0.93,p<0.01) and curvilinear regression equation (Y=0.6273+3.0883D-0.7485D²,R=0.96,p<0.05) has been obtained using regression analysis methods. γ-ray can induced CDKN1A gene expression changed and increase dose-dependently, but the stabilityof regression equation should be tested further.

Key words: Ionizing radiation, Human peripheral blood, CDKN1A, Gene expression