高能中子辐射对线虫的剂量效应研究

Abstract

Abstract: This paper studies the dose effect of high-energy neutron radiation on Caenorhabditis elegans and discusses the relative biological effects of high-energy neutron radiation. Hermaphrodite C.elegans were randomly divided into control group and 10 irradiation dose groups of 1 047, 476, 199, 89, 18.2, 8.4, 1.83, 0.351, 0.171 and 0.087 Gy, respectively. C.elegans in each group are placed in different distance from neutron source accordingly with same irradiation time. After a single high-energy neutron whole-body irradiation, part of C.elegans were transferred into the new dish immediately for follow-up detection of spawning rate and lifespan. The other part were transferred 24 hours later for germ cell death detection. The results showed that the spawning rate presented a general decline trend with the increase of dose, especially the damage of 1.83 Gy irradiation on the number of C.elegans progeny. C.elegans with more than 89 Gy radiation stopped spawning. The lifespan of C.elegans showed a trend of decreasing with the increase of radiation dose, particularly the shortening effect of 1.83 Gy was obvious. When irradiated C.elegans with greater than 8.4 Gy, the germ cell death rised significantly with the increase of dose. The above results show that high-energy neutron radiation has a dose effect on C.elegans. There might be a stronger damage effect at low dose, which provides scientific basis for neutron low-dose radiation protection. In this paper, it is also discussed that the relative biological effect of HINEG high-energy neutron radiation is 1.25, which is calculated by comparing the neutron irradiated data with the previous γ experimental results. It showed the biological effect difference between γ and neutron irradiation. The quality factor(Q)we suggested is different from that of ICRP report. It still needs more effort to improve the reference animal database.

Key words: neutron, Caenorhabditis elegans, biological effectiveness, low-dose hyper-radiosensitivity

CLC Number:

Q691

XU Zhao, CHEN Ni, WANG Zhigang, LI Taosheng. Dose effect of high-energy neutron radiation on Caenorhabditis elegans[J].RADIATION PROTECTION, 2019, 39(2): 150-156.

References

[1] 夏寿萱. 放射生物学[M]. 北京:军事医学科学出版社, 1998.
XIA Shouxuan.Radiation biology[M]. Beijing: Military Medical Science Press,1998.
[2] 段志凯, 郭万龙, 刘建功. 中子辐射生物效应研究进展[J]. 辐射防护通讯, 2008, 28(5):19-22.
DUAN Zhikai, GUO Wanlong, LIU Jiangong, et al. Development of neutron-induced biological effectiveness research [J]. Radiation Protection Bulletin, 2008, 28(5):19-22.
[3] 周永茂. 迈入新世纪的硼中子俘获疗法(BNCT)[J]. 中国工程科学, 2012, 14(8):4-13.
ZHOU Yongmao. The boron neutorn capture therapy (BNCT) status to go into the new era [J]. Engineering Sciences, 2012, 14(8):4-13.
[4] WU C, Bordeos A, Madamba M R S, et al. Rice lesion mimic mutants with enhanced resistance to diseases[J]. Molecular Genetics & Genomics, 2008, 279(6):605-19.
[5] Bruggemann E, Handwerger K, Essex C, et al. Analysis of fast neutron-generated mutants at the Arabidopsis thaliana HY4, locus[J]. Plant Journal, 1996, 10(4):755-760.
[6] Henikoff S, Comai L. Single-nucleotide mutations for plant functional genomics[J]. Annual Review of Plant Biology, 2003, 54(1):375-401.
[7] Edgley M, D’Souza A, Moulder G, et al. Improved detection of small deletions in complex pools of DNA[J]. Nucleic Acids Research, 2002, 30(12):e52.
[8] Sasaki M S, Endo S, Hoshi M, et al. Neutron relative biological effectiveness in Hiroshima and Nagasaki atomic bomb survivors: a critical review[J]. Journal of Radiation Research, 2016, 57(6):583-595.
[9] Baiocco G, Barbieri S, Babini G, et al. The origin of neutron biological effectiveness as a function of energy[J]. Scientific Reports, 2016, 6(6):34 033.
[10] Phillips T L, Fu K K. Biological of 15 MeV neutrons[J]. International Journal of Radiation Oncology Biology Physics, 1976,1(11-12):1 139.
[11] 王晶晶, 许安, 代慧, 等. 利用模式生物秀丽线虫评价磁场生物效应进展[J]. 应用与环境生物学报, 2015, 21(6):1 003-1 011.
WANG J J, XU An, DAI H, et al. Progress in assessing biological effects of magnetic fields using model organism progress in assessing biological effects of magnetic Caenorhabditis elegans[J]. Chinese Journal of Applied & Environmental Biology, 2015, 21(06):1 003-1 011
[12] WU Y C. Development of high intensity D-T fusion neutron generator HINEG[J].International Journal of Energy Research,2016, 42(1):68-72.
[13] 吴宜灿,刘超,宋钢,等. 强流氘氚聚变中子源HINEG设计研究[J].核科学与工程, 2016, 36(1):77-83.
WU Y C, LIU C, SONG G, et al. Design study of high intensity D-T fusion neutron generator HINEG[J]. Nuclear Science and Engineering, 2016, 36(1):77-83.
[14] 王刚,于前锋,王文,等. 10～(12)n/s氘氚聚变中子发生器旋转氚靶设计与传热分析[J].核科学与工程.2015, 64(1):13-17.
WANG G, YU Q F, WANG W, et al. Design and heat transfer analysis of rotating tritium target of 10～(12)n/s D-T fusion neutron generator[J]. Nuclear Science & Engineering, 2015, 64(1):13-17.
[15] 田志恒,潘自强.辐射剂量学[M].北京:原子能出版社,1992.
TIAN Zhiheng, PAN Ziqiang.Radiation dosimetry[M].Beijing: Atomic Energy Press,1992.
[16] 李星洪.辐射防护基础[M].北京:原子能出版社,1982.
LI Xinghong.Basis of radiation protection[M].Beijing: Atomic Energy Press,1982.
[17] 刘家炉, 郭肖颖, 黎青青, 等. 碳离子辐射诱导秀丽隐杆线虫生殖细胞凋亡研究[J]. 原子核物理评论, 2015, 32(1):105-109.
LIU Jialu, GUO Xiaoying, LI Qingqing, et al. Effects of carbon ion irradiation on the germ cell apoptosis in Caenorhabditis elegans[J]. Nuclear Physics Review, 2015, 64(1):13-17.
[18] 黎青青. 线虫辐射损伤信号空间转导与机制研究[D].中国科学技术大学,2017.
LI Qingqing. The spatial function and the mechanism of damage signal transduction in Caenorhabditis elegans[D]. University of Science and Technology of China,2017.
[19] Marples B, Krueger S A, Collis S J, et al. Low dose hyper-radiosensitivity: a historical perspective [M]//Targeted Radionuclide Tumor Therapy. Springer, Dordecht, 2008: 329-347.
[20] 李德平. ICRP和辐射防护的发展——祝贺ICRP诞生70周年[J]. 辐射防护, 1998, 18(Z1):130-138.
LI Deping. Progress of ICRP and radiation protection:celebration of ICRP 70th [J]. Radiation Protection, 1998, 18(Z1):130-138.
[21] 叶侃,辐射对秀丽线虫的生长损伤及其防护研究[D]. 苏州大学,2011
YE Kan. Study on the radiation damage of growth and the protection in Caenorhabditis elegans[D]. Soochow University,2011.
[22] ICRP. 1990 Recommendations of the International Commission on Radiological Protection[M]. ICRP Public 60. Oxford: Pergamon Press, 1991.
[23] А.Г.斯维尔德洛夫. 中子的生物效应和化学防护[M]. 北京:原子能出版社, 1981.
Свердлов А Г. Biological effect and chemical protection of neutron[M]. Beijing: Atomic Energy Press, 1981.
[24] ICRP. The 2007 Recommendations of the International Commission on Radiological Protection[M]. ICRP Public 103. Oxford: Pergamon Press, 2007.

Dose effect of high-energy neutron radiation on Caenorhabditis elegans

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