单细胞单粒子微束的发展及放射生物学应用现状

Abstract

Abstract: The latest development of single-cell single-particle microbeam and its applications in radiobiology are reviewed. Particle beam from accelerator can be collimated or be focused into micrometer or sub-micrometer size in the air. Focused microbeam has become the mainstream of development due to its higher spatial resolution and faster electromagnetic scanning irradiation throughput. Coupling with advanced fluorescence microscopy and time-lapse imaging, the current microbeam can visualize the early response of living cell to radiation induced DNA damage online. Based on the characteristics of targeted irradiation individual cell or subcellular compartment with precise controlled particle number, microbeam has become a unique experimental research tool for low dose effect, radiation induced bystander effect and radiation sensitivity of subcellular compartment. Its applications are gradually expanding from cell culture model to more complex tissue or in vivo system in order to investigate the spatial and temporal evolution of radiation response of irradiated biological samples.

Key words: single-cell single-particle microbeam;particle accelerator;sub-micrometer resolution;radiation biology

CLC Number:

Q691

CHEN Faguo, LIN Haipeng, WANG Yong, DANG Xuhong, LIANG Runcheng, REN Yue. Development of single-cell single-particle microbeam and applications in radiobology[J].RADIATION PROTECTION, 2022, 42(3): 184-192.

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Development of single-cell single-particle microbeam and applications in radiobology

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