电离辐射对断乳期小鼠脾脏发育的影响研究

Abstract

Abstract: This experiment investigates the effects and mechanism of ionizing radiation on spleen development of mice during weaning age. Totally fourteen C57 mice with 21-days-old were randomly divided into two groups (control group and irradiation group, each with seven mice). The mice in the irradiation group have received the whole body X-ray radiation with the dose of 5 Gy. The samples were taken from mouse of each group at seven days after radiation respectively. The body weight and spleens were weighed and calculated spleen indicators. Biochemical method was utilized to detect the changes of peripheral blood of mice. Immunohistochemistry and western blotting were used to detect the protein expression of phospho-H₂AX in spleen. Compared to the control group,the body weights and the spleen weights of mice in the irradiation group were significantly reduced (p<0.01), indicating that radiation can significantly reduce the content of white blood cells, platelets, red blood cells and hemoglobin, as well as the expression of phospho-H₂AX (p<0.05, p<0.01). X-ray exposure could resulted abnormal functions of the spleen in weaning age mice, which may be related to the decreased expression of p-H₂AX, thus reducing the ability of DNA repair.

Key words: X-ray, spleen, H₂AX, DNA damage

CLC Number:

Q691

ZHENG Yudong, LIU Yuanduo, WANG Changzheng, HUANG Jiangrong, LIU Lian. Effect of ionizing radiation on spleen development of mice during weaning age[J].RADIATION PROTECTION, 2021, 41(5): 467-472.

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Effect of ionizing radiation on spleen development of mice during weaning age

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