低剂量辐射对小鼠肠道损伤的影响

Abstract

Abstract: Objective: To compare the changes of intestinal injury indexes in mice exposed to different low-dose radiation, so as to provide scientific basis for the establishment of intestinal injury model in mice exposed to low-dose radiation.Methods: Balb/c mice in three groups were irradiated with three different low dose rates (0.04 Gy/d, 0.12 Gy/d, and 0.2 Gy/d) for five consecutive days (cumulative exposure of 0.2, 0.6, and 1.0 Gy, respectively), and a control group (NC) was established. After the irradiation, the body weight, peripheral blood picture, organ index, malondialdehyde (MDA) content in small intestinal tissue, superoxide dismutase (SOD) activity, immune cytokine (IL-1β, IL-2, IL-6, TNF-α), DNA damage were determined, to explore the optimal dose of low dose radiation for intestinal injury in mice.Results: After five days of continuous irradiation, compared with the control group, the body weight and organ index of mice in each irradiation group were decreased to different extents (p< 0.05 in the spleen index L group, p< 0.01 in the M and H groups). MDA content in small intestinal tissue was significantly increased (p< 0.05 in Group L, and p< 0.01 in Group M and H); SOD content was decreased to different degrees. TNF-α, IL-2, IL-1β and IL-6, the typical proinflammatory factors, showed dose-dependent increases (IL-1β: M, group H p< 0.05, IL-2: M group p< 0.05, group H p< 0.01, TNF-α: M, group H p< 0.05). Group M and H had significant DNA damage and apoptosis.Conclusion: The optimal dose and method for low dose rate radiation induced intestinal injury in mice is 0.12 Gy/d continuous irradiation and 0.6 Gy accumulation for 5 days.

Key words: low dose radiation, model, mouse, intestinal

CLC Number:

R818

HU Changkun, ZHANG Xuemei, MA Zenchun, GAO Yue. Effects of low dose radiation on intestinal injury in mice[J].RADIATION PROTECTION, 2021, 41(4): 315-320.

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Effects of low dose radiation on intestinal injury in mice

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