海带多糖通过促进Sirt3/MnSOD表达来改善受照射后小鼠的下颌下腺损伤

Abstract

Abstract: The aim of this study is to investigate protective effect and the underlying mechanism of Laminaria Japonica Polysaccharide (LJP) on radiation-induced submandibular gland injury in mice. Eight-week-old female mice were randomly divided into four groups, including control group, LJP group, irradiation group, and irradiation plus LJP group. LJP (100 mg/kg/day) was intraperitoneally injected 3 days before irradiation and for consecutive 7 days (once daily).The mice from irradiation alone and irradiation plus LJP groups were exposed to a single dose of 15 Gy by using the ⁶⁰Co gamma rays. Histopathology and function of the submandibular glands (SMG) were detected on the 28th day post irradiation. The protein and mRNA expression of Sirt3 and MnSOD were respectively examined by real-time qPCR and immunohistochemical staining methods. Results showed that LJP injection significantly increased saliva flow rates in irradiated mice, improved the morphology and structure of SMG, and markedly up-regulated expressions of the Sirt3 and MnSOD. This suggests that LJP treatment could alleviate radiation-induced submandibular gland injury through activation of the Sirt3/MnSOD pathway.

Key words: Laminaria Japonica Polysaccharide, submandibular gland, Sirtuin3, manganese superoxide dismutase, radiation-induced xerostomia

CLC Number:

R142+.4

ZHANG Siqin, AO Pian, CHEN Shaoyong, CHAI Rong, WANG Shaojun, WEI Li, XU Yang, CHEN Chen. Laminaria Japonica Polysaccharide attenuates radiation-induced submandibular gland injury in mice via promoting Sirt3/MnSOD expression[J].RADIATION PROTECTION, 2021, 41(3): 264-270.

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Laminaria Japonica Polysaccharide attenuates radiation-induced submandibular gland injury in mice via promoting Sirt3/MnSOD expression

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