Abstract: The protective effect of hydrogen rich water on the acute hematological damage of Beagle dogs exposed to ⁶⁰Co γ ray irradiation was studied. Male Beagle dogs were randomly divided into control group, irradiation group and hydrogen rich water intervention group. After a single irradiation of 2.0 Gy gamma ray, the peripheral blood of the animals was collected on the 2^nd and the 7^th day. The indexes of hematology, antioxidation, lymphocyte apoptosis rate and 8-OHdG were detected. The results showed that on the 7^th day after irradiation, RBC, HGB and HCT of the hydrogen rich water group were higher than those of the irradiation group (p<0.01, p<0.05, p<0.05); on the 2^nd day and the 7^th day after irradiation, T-AOC of the hydrogen rich water group was higher than that of the irradiated group (p<0.01, p<0.05), and MDA was lower than that of the irradiation group (p<0.01, p<0.05); on the 2^nd day and the 7^th day after irradiation, lymphocyte apoptosis rate of the hydrogen rich water group was lower than that of the irradiation group (p<0.05, p<0.01); on the 7^th day after exposure, the concentration of 8-OHdG in hydrogen rich water group was lower than that of the irradiation group (p<0.05). These results indicate that hydrogen rich water can improve the blood index of Beagle dogs by the mechanism of anti oxidative stress and anti apoptosis.

Key words: hydrogen rich water, Beagle dog, gamma ray, hematological parameter, radiation protection