Abstract: To study the irradiation effects at different doses (0.1, 0.2, 0.5,1.0,2.0,4.0 Gy) and different time (6,12,24 h) on the expression of BMP pathway related genes in human hepatocytes by real-time quantitative PCR. Results Solution curves of β-actin, BMP2, BMP7, BMPR1A, BMPR1B, and BMPR2 with different doses and time were single peak, showed the specific amplification. The expression levels of BMP2 were positively correlated with time; the expression levels of BMP7 were negatively correlated with time at doses of 0.1, 0.2, and 0.5 Gy, and positively correlated with dose at 12 and 24 h after irradiation; the expression levels of BMPR1A were positively correlated with time at doses of 0.1, 0.2, and 1.0 Gy; the expression levels of BMPR1B were positively correlated with time at doses of 0.2, 0.5, and 1.0 Gy, and negatively correlated with time at dose of 4.0 Gy; the expression levels of BMPR2 were negatively correlated with dose at 12 h after irradiation.

Key words: human hepatocytes, gamma ray, BMP pathway, gene expression