60Co γ射线照射人肝癌细胞诱导PIF1基因mRNA变化

RADIATION PROTECTION ›› 2016, Vol. 36 ›› Issue (3): 149-153.

Previous Articles Next Articles

Changes of PIF1 gene expression induced by ⁶⁰Co γ ray in HepG2

Wang Bin^1,3, Zhang Ying^2,3, Zhao Degen⁴, Li Zhongqiu^2,3, Yang Yang^2,3, Li Chao^1,3, Zhu Maoxiang³, Gu Yongqing³

1. College of Life Sciences Shihezi University,Xinjiang Shihezi 832003;
2. College of Medicine Shihezi University, Xinjiang Shihezi 832003;
3. Institute of Radiology and Radiation Medicine,the Chinese Academy of Military Medicine, Beijing 100850;
4. Bengbu Third People's Hospital,Anhui Bengbu 233000

Received:2016-01-11 Online:2016-05-20 Published:2021-11-12

Abstract

Abstract: HepG2 cells were irradiated by 2 Gy and 4 Gy ⁶⁰Co γ ray, the mRNA was extracted and real-time PCR was used to detect the changes of PIF1 mRNA, to explore whether PIF1 involved in DNA damaged respond after irradiation. The results showed that PIF1 gene expression level in HepG2 decreased slightly at first and then increased after different doses of ⁶⁰Co γ ray, and the high expression continued about 12 h. PIF1 gene expression can be induced by ionizing radiation indicating its roles in DNA damage response, and the expression level is related to radiation doses and time.

Key words: PIF1 helicase, ionizing radiation, DNA damage and repair, real-time PCR

CLC Number:

Q344

Wang Bin, Zhang Ying, Zhao Degen, Li Zhongqiu, Yang Yang, Li Chao, Zhu Maoxiang, Gu Yongqing. Changes of PIF1 gene expression induced by ⁶⁰Co γ ray in HepG2[J].RADIATION PROTECTION, 2016, 36(3): 149-153.

References

[1] Matson SW, George JW, Bean DW. DNA helicases:enzymes with essential roles in all aspects of DNA metabolism[J]. Bioessays, 1944, 16(1):13-22.
[2] Hanada K, Hickson ID. Molecular genetics of RecQ helicase disorders[J]. Cell Mol Life Sci, 2007, 64(17):2 306-2 322.
[3] Bochman ML, Sabouri N, Zakian VA. Unwinding the functions of the PIF1 family helicases[J]. DNA Repair (Amst), 2010, 9(3):237-249.
[4] Shimada K, Gasser SM. DNA replication:PIF1 pulls the plug on stalled replication forks[J]. Curr Biol, 2012, 22(10):404-405.
[5] Wang J, Englund PT, Jensen RE.TbPIF8,a trypanosomabrucei protein related to the yeast Pif1 helicase, isessentialfor cell viability and mitochondrialgenome maintenance[J]. Molmicrobiol, 2012, 83(3):471-485.
[6] Schuldt A. DNA replication: Pif1 overcomes a quadruplex hurdle[J]. Nat Rev Mol Cell Biol, 2011, 12(7):402-408.
[7] Maier P, Hartmann L, Wenz F, et al. Cellular pathways in response to ionizing radiation and their target ability for tumor radiosensitization[J]. Int J Mol Sci,2016 , 17(1):1-32.
[8] Ceccaldi R, Rondinelli B,Dandrea AD. Repair pathway choices and consequences at the double-strand break[J]. Trend Cell Biol,2015, 26(1):52-64.
[9] Makovets S, Blackburn EH. DNA damage signaling prevents deleterious telomere addition at DNA breaks[J]. Nat Cell Biol,2009, 11(11): 1 383-1 386.
[10] Pike JE, Burgers PM, Campbell JL, et al. Pif1 helicase lengthens some Okazaki fragment flaps necessitating DNA2 nuclease/helicase action in the two nuclease processing pathway[J]. J Biol Chem,2009, 284(37): 25 170-25 180.
[11] Gu YQ, Masuda Y, Kenji K. Biochemical analysis of human PIF1 helicase and functions of its N-terminal domain[J]. Nucleic Acid Res, 2008, 36(19): 6 296-6 308.
[12] 顾永清. 人类重组PIF1蛋白的表达纯化和解螺旋酶活性的分析[J]. 生物工程学报, 2009, 25(2):268-273.
[13] 顾永清. 不含氨基末端的PIF1解螺旋酶的纯化及初步功能研究[J]. 西安交通大学学报(医学版), 2009, 30(3): 274-276.
[14] 李珊珊.人PIF1及其截短体重组蛋白的真核表达及其细胞内定位[J]. 西安交通大学学报(医学版), 2014, 35(5):595-598.

Changes of PIF1 gene expression induced by ⁶⁰Co γ ray in HepG2

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 5

Metrics

Comments

Recommended 0

[1]	HUANG Yue, CHEN Naiyao, ZHAO Hui, YAN Zhenyu, ZHANG Haixia, ZHAO Xuecong, ZHANG Dingping. Effects of N-acetylcysteine on oxidative stress, proliferation and apoptosis of HT22 cells induced by radiation [J]. RADIATION PROTECTION, 2021, 41(2): 165-173.
[2]	WANG Chengfang, SHAO Shuai, QU Gonglin, QI Xuesong, GOU Qiao. The protection effect of Sijunzi Decoction (Four Noble Drugs Decoction) on radiation damage of human intestinal epithelial cells [J]. RADIATION PROTECTION, 2020, 40(5): 456-461.
[3]	GAO Fei, CHEN Dongfeng, XIAO Xuefu, XU Yang. Study on pulsed X ray response characteristics of active ionization radiation dosimeters [J]. RADIATION PROTECTION, 2019, 39(6): 469-474.
[4]	DANG Xuhong, ZUO Yahui, WANG Fang, ZHANG Jingyun, DONG Juancong, ZHANG Zhongxin, DUAN Zhikai. Detection and analysis of MXR7 gene expression in human liver cells induced by radiation and peripheral blood of radiation workers [J]. RADIATION PROTECTION, 2018, 38(6): 517-521.
[5]	Li Chao, Li Zhongqiu, Li Xueping, Yang Yang, Zeng Yan, Pan Xiujie, Yang Zhihua, Zhu Maoxiang, Gu Yongqing. Effects of salt-inducible kinase 2 on autophagy and apoptosis induced by ionizing radiation [J]. RADIATION PROTECTION, 2017, 37(3): 214-222.

Changes of PIF1 gene expression induced by 60Co γ ray in HepG2

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 5

Metrics

Comments

Recommended 0

Changes of PIF1 gene expression induced by ⁶⁰Co γ ray in HepG2