137Cs γ 射线累积照射SD大鼠后肝脏蛋白质组的分析

Abstract

Abstract: This study was designed to investigate the changes of proteomics in liver of Sprague-Dawley(SD)rats after accumulated irradiation by ¹³⁷Cs γ ray. Isobaric tags for relative and absolute quantitation (iTRAQ) was used to analyze the different protein expression in liver of irradiated rats. Thirty mature SD rats were randomly divided into three groups: 0.2 Gy group, 2 Gy group and healthy control group. Rats were irradiated at dose rate of 0.336 mGy/min for 10 days and 20 days continuously. Gene Ontology and protein-protein interaction network analysis were conducted using softwares. The results demonstrated that 2 220 protein spots were identified. 122 proteins were differentially expressed both in two groups compared with control, of which 52 were up-regulated and 70 down-regulated. Based on the information of GO categories,these differentially expressed proteins mainly located in the cytoplasm and membrane,involving in the function of protein binding and catalytic activity. Analysis with the PAJEK software demonstrated that those 16 differentially expressed proteins could form a complicated interaction network where SF3B1, ATP6V1C1 might be key nodes.

Key words: accumulated irradiatin, proteomics, isobaric tags for relative and absolute quantitation (iTRAQ), interaction network

CLC Number:

Q691

Zuo Yahui, Dang Xuhong, Yuan Yayi, Wang Chao, Zhang Zhongxin, Liu Jiangong, Zhang Ruifeng, Duan Zhikai. Study on liver proteomics of rats accumulated ¹³⁷Cs γ ray irradiation[J].RADIATION PROTECTION, 2015, 35(2): 87-92.

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Study on liver proteomics of rats accumulated ¹³⁷Cs γ ray irradiation

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Study on liver proteomics of rats accumulated 137Cs γ ray irradiation

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Study on liver proteomics of rats accumulated ¹³⁷Cs γ ray irradiation