γ射线照射人肝细胞对胰岛素样生长因子相关基因表达的影响

Abstract

Abstract: The study analyzed the effect-relationship of γ-ray irradiation with different doses (0.1, 0.2, 0.5, 1, 2 and 4 Gy) and different times(6, 12 and 24 h) of IGF related genes in human hepatocytes by real-time fluorescent quantitative PCR. The results indicated that insulin-like growth factor-1 receptor (IGF1R) showed low expression with different dosage and duration; insulin-like growth factor binding protein-1 (IGFBP1) showed high expression after radiation 6 h, 24 h showed low expression, 0.1 and 0.2 Gy showed high expression and 0.5, 1, 2 and 4 Gy low expression after radiation 12 h; IGF2BP3 showed low expression with 0.2 Gy and 6 h of 0.5 Gy, and high expression with other dosage and duration; IGFBP4 showed low expression with 6 h, and high expression with 12 and 24 h.

Key words: gamma-ray, human hepatocytes, insulin-like growth factor (IGF), real-time fluorescent quantitative PCR

CLC Number:

Q343

Li Jianguo, Yuan Hui, Yin Jingjing, Qin Xiujun, Zhang Wei, Wen Jianhua. Study of γ-ray Irradiation with Expressive Influence of IGF Related Genes in Human Hepatocytes[J].RADIATION PROTECTION, 2014, 34(2): 102-108.

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Study of γ-ray Irradiation with Expressive Influence of IGF Related Genes in Human Hepatocytes

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