基于元素分析仪的生物基体碳含量分析

doi:10.27045/j.1000-8187.202603006

Abstract

Abstract: ¹⁴C,a long-lived radionuclide, is widely used as a tracer in human mass balance and pharmacokinetic studies. The ¹⁴C/¹²C ratio in biological samples was measured by a high-sensitivity accelerator mass spectrometer, and the carbon content in the samples was analyzed by the elemental analyzer, which can reflect the content of ¹⁴C markers in different biological matrices ( including serum, plasma, whole blood and urine). In this paper, a high-precision measurement program for carbon content in biological samples was established by studying the key parameters of carbon content in biological samples measured by the elemental analyzer. The results show that the relative standard deviation of carbon content measurement in various biological samples is less than 5% when the sample sampling amount is 60~100 mg and the oxygen flow is ≥75 mL. It can be applied to the high-precision analysis of carbon content in different types of biological samples, and provides an effective analytical method for the accurate calculation of ¹⁴C activity in subsequent samples.

Key words: carbon content, biological samples, elemental analyzer

CLC Number:

X830.2

HUANG Hui, YUAN Ni, AN Quan, LUO Maoyi, WANG Zhipeng, GUO Yuanyuan, WANG Yuanfei. Determination of carbon content in biological samples using elemental analyzer[J].RADIATION PROTECTION, 2026, 46(3): 234-242.

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Determination of carbon content in biological samples using elemental analyzer

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