低剂量氚照射对仔鼠中枢神经系统的影响及其机理研究

Abstract

Abstract: Effects of low-dose tritium on thecentral nervous system of offspring mice and rats were studied. C57BL/6J mice and Wistar ratswere given single intraperitoneal injection of tritiated water, at doses of 0.00, 24.09, 48.18 and 144.54 (×10⁴ Bq/g) on the 12.5th and 13th day of gestation respectively. The results showthat pre-natal tritium irradiation leads to: (1)hindered growth and development after birth, a transform of neurobehavioral behavior from the early excitatory state to a late inhibition state, significantly reducedlearning ability and memory function; (2) thinning brain and cerebellar cortex thickness, decreased number of pyramidal cells, markedly decreased number and ratio of primary and secondary dendrites, decreased number of hippocampal pyramidal cells and loss of brain neuron cells; (3)increased pituitary somatostatin and decreased hypothalamic arginine vasopressin; (4)decreased Ca²⁺ current, increased apoptosis, increased DNA ladder map and p53 protein expression for developing neurons; (5)teratogenic thresholds of 0.03-0.092 Gy for 45 biological indicators, and 0.093-0.30 Gy for the other 11 ones studied concurrently. It is concluded that the material basis of central nervous system damage caused by tritium radiation is mainly the change of brain neuroactive substances, the decrease of Ca²⁺ current amplitude of hippocampal neurons, the increase of brain cell apoptosis, and the abnormal expression of p53 protein.

Key words: Tritium, Mouse, Rat, Central nerve system, Hippocampus, Ca²⁺ current, Apoptosis, Threshold dose

CLC Number:

Q345+.1

Wang Bing, Lyu Huimin, Liu Yulong, Gao Weimin, Wang Chunyan, Dong Jinchan, Zhang Wei, Chen Weibo. Effects of Low-dose Tritium Irradiation onthe Central Nervous System of Offspring Rats and Its Mechanism[J].RADIATION PROTECTION BULLETIN, 2019, 39(5): 2-18.

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Effects of Low-dose Tritium Irradiation onthe Central Nervous System of Offspring Rats and Its Mechanism

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