辐射旁效应及其机制研究进展

摘要/Abstract

摘要： 辐射旁效应是未受辐射的细胞表现出与受辐射的细胞相同或相似的生物学反应的现象。本文简要介绍了生物体外、体内辐射旁效应的研究现状,简述辐射旁效应中有关时间-效应方面的研究,以及辐射旁效应产生机制的研究进展。

关键词: 辐射旁效应, 体内研究, 体外研究, 细胞间隙连接通讯, 细胞介质

Abstract: Radiation induced bystander effect isa phenomenon that non-radiated cells have the same or similar biological reaction with those radiated cells. A large number of studies show the objective existence of this effect. The paper briefly reviews its research status, in vitro andin vivo, and discusses studies on time-effect and research progress of generation mechanism in the respect.

Key words: Radiation induced bystander effect, Study in vivo, Study in vitro, Gap junction intercellular communication, Cell medium

中图分类号:

R818.03

张朝宁, 李金田, 刘永琦. 辐射旁效应及其机制研究进展[J]. 辐射防护通讯, 2015, 35(3): 19-24.

Zhang Chaoning, Li Jintian, Liu Yongqi. Advances in Research of Radiation Induced Bystander Effect and Its Mechanism[J]. RADIATION PROTECTION BULLETIN, 2015, 35(3): 19-24.

参考文献

[1] Li P T. Low Concentration of Exogenous Carbon Monoxide Protects Mammalian Cells against Proliferation Induced by Radiation-induced Bystander Effect[J]. Mutation Research, 2014,759(2):9.
[2] Najafi M, Fardid R, Hadadi G, et al. The Mechanisms of Radiation-Induced Bystander Effect[J]. Journal of Biomedical Physics Engineering,2014,15(4):163.
[3] PARSONS W B Jr, WATKINS C H, PEASE G L, et al. Changes in Sternal Marrow Following Roentgen-ray Therapy to The Spleen in Chronic Granulocytic Leukemia[J]. Cancer Letter, 1954,7(1):179.
[4] Littlefield L G, Hollowell J J, Pool W J, et al. Chromosomal Aberrations Induced By Plasma From Irradiated Patients: an Indirect Effect ofX Radiation. Further Observations and Studies of a Control Population[J]. Radiology, 1969,93(4):879.
[5] Demoise C F, Conard R A. Effects of Age and Radiation Exposure on Chromosomes in a Marshall Island Population[J]. Journal of Gerontology,1972,27(2):197.
[6] Nagasawa H, Little J B. Induction of Sister Chromatid Exchanges by Extremely Low Doses of alpha-Particles[J]. Cancer Research, 1992,52(22):6394.
[7] 王转子, 李文建, 魏巍, 等. 电离辐射旁效应的研究方法概述[J]. 辐射研究与辐射工艺学报, 2009,27(4):193.
[8] 余思逊, 海春旭,梁欣, 等. ⁶⁰Co γ射线辐照肿瘤细胞对未照射细胞的旁观者效应及Trolox的干预作用[J]. 癌变·畸变·突变, 2008,2(3):212.
[9] 孙大庆, 肖瑶, 韩玲, 等. AP1S1在辐射旁效应中高表达及其在旁效应中的作用[J]. 辐射防护, 2010,30(6):356.
[10] 贾荣飞, 陈红红, 于雷, 等. ¹²⁵I籽源持续低剂里率照射诱导人肺癌细胞的旁效应[J]. 辐射研究与辐射工艺学报, 2007,25(6):363.
[11] 郭鲜花, 涂序珉, 左雅慧, 等. ⁶⁰Co γ射线照射K562细胞后培养基转移介导的旁效应研究[J]. 辐射防护, 2009,29(2):86.
[12] 陈纤, 蒋友芹, 尹晓明, 等. 外泌体—电离辐射诱导旁效应的另一种机制[J]. 辐射研究与辐射工艺学报, 2014,32(3):1.
[13] Mothersill C, Seymour C B. Cell-Cell Contact During Gamma Irradiation Is Not Required to Induce a Bystander Effect in Normal Human Keratinocytes:Evidence for Release During Irradiation of a Signal Controlling Survival Into the Medium[J]. Radiation Research, 1998,149(4):256.
[14] Yang H, Anzenberg V, Held K D, et al. The Time Dependence of Bystander Responses Induced By Iron-Ion Radiation in Normal Human Skin Fibroblasts[J]. Radiation Research, 2007,168(3):292.
[15] 杜亚蓉, 潘冬, 陈亚雄, 等. 一氧化氮合成酶高表达促进辐射旁效应的产生[J]. 辐射研究与辐射工艺学报, 2013,31(2):1.
[16] Khan M A, Van Dyk J, Yeung I W, et al. Partial Volume Rat Lung Irradiation: Assessment of Early DNA Damage in Different Lung Regions and Effect ofRadical Scavengers[J]. Radiotherapy and Oncology, 2003,66(1):95.
[17] Koturbash I, Rugo R E, Hendricks C A, et al. Irradiation Induces DNA Damage and Modulates Epigenetic Effectors in Distant Bystander Tissue in Vivo[J]. Oncogene, 2006,25(31):4267.
[18] 杨龙, 王奕萍, 荣曙, 等. ⁶⁰Coγ射线左半身照射对小鼠非照射区域骨髓造血组织VEGF表达的影响[J]. 中国老年学杂志, 2007,27(4):643.
[19] 陈凤, 张凤翔. 电离辐射诱发胎鼠的形态及胎脑组织甲基化结合蛋白MeCP2改变[J]. 中国辐射卫生, 2012,21(1):13.
[20] Raiche J, Rodriguez-Juarez R, Pogribny I, et al. Sex-and Tissue-Specific Expression of Maintenance and de novo DNA Methyltransferases upon LowDose X-Irradiation in Mice [J]. Biochemical and Biophysical Research Communications, 2004,325(1):39.
[21] Pogribny I, Raiche J, Slovack M, et al. Dose-Dependence,Sex-andTissue-Specificity, and Persistence of Radiation-Induced Genomic DNA Methylation Changes[J]. Biochemical and Biophysical Research Communications, 2004,320(4):1253.
[22] Koturbash I, Kutanzi K, Hendrickson K, et al. Radiation-Induced Bystander Effects in Vivo are Sex Specific[J]. Mutation Research, 2008,642(1-2):28.
[23] Yang S, Xu J, Shao W, et al. Radiation-Induced Bystander Effectsin A549 Cells Exposed to 6 MV X-rays[J]. Cell Biochemical and Biophysics, 2015,17(2):221.
[24] 杨剑, 韩玲, 高娴. PTEN在电离辐射诱导旁效应细胞中的表达变化[J].第二军医大学学报, 2009,302:137.
[25] 高娴, 孙顶, 杨剑, 等. γ射线致旁效应细胞脆性组氨酸三联体的表达[J]. 中华放射与防护杂志, 2008,28(3):217.
[26] 崔大超, 李强, 吴庆丰, 等. C离子辐照引起细胞分泌旁效应信号因子浓度随时间的变化[J]. 原子核物理评论, 2012,29(2):189.
[27] Azzam E I, de Toledo S M, SpitzD R, et al. Oxidative Metabolism Modulates Signal Transduction and Micronucleus Formation in Bystander Cells from alpha-Particle-Irradiated Normal Human Fibroblast Cultures[J]. Cancer Research, 2002,62(19):5436.
[28] Azzam E I, de Toledo S M, Little J B, et al. Direct Evidence for The Participation of Gap Junction-Mediated Intercellular Communication in The Transmission of Damage Signals from alpha -Particle Irradiated to Non-irradiatedCells[J]. Proceedings of the National Academy of Sciences of the United States of America, 2001,98(2):473.
[29] 张蕾蕾, 吴李君, 韩伟, 等. 应用hprt和CD59基因研究辐射旁效应的产生及机理[J]. 中国科学院研究生院学报, 2006,23(6):753.
[30] Autsavapromporna N, Suzukia M. Gap Junction Communication and thePropagation of Bystander Effects Induced by Micro-beam Irradiation in Human Fibroblast Cultures: The Impact of Radiation Quality[J]. Radiation Research, 2013,180(4):367.
[31] Desai S, Kobayashi A, Konishi T, et al. Damaging and Protective Bystander Cross-talk Between Human Lung Cancer and Normal Cells after Proton Micro-beam Irradiation[J]. Mutation Research, 2014,763(4):39.
[32] Belchior A, Balásházy I, Monteiro Gil O, et al. Dose the Numberof Irradiated Cells Influence the Spatial Distribution of Bystander Effects[J]. Dose-response, 2014,12(4):525.
[33] 赵镁嘉, 沈波, 邵春林. 电离辐射旁效应信号因子的研究进展[J]. 中华放射医学与防护杂志, 2008,28(3):310.
[34] Little J B, Azzam E I, de Toledo S M. Nagasawa H Bystander Effects: Intercellular Transmission of Radiation Damage Signals[J]. Radiation Protection Dosimetry, 2002,99(1-4):159.
[35] 刘文文. 沙眼衣原体感染诱生的活性氧(ROS)对宿主细胞坏死的影响[J].复旦学报(医学版), 2012,39(4):385.
[36] 涂序珉, 雷苏文, 王菊芳, 等. 用培养基介导法研究辐射旁效应及其机理[J]. 中华放射医学与防护杂志, 2005,25(4):333.
[37] Wang X, Zhang J, Fu J, et al. Role of ROS-mediated Autophagy in Radiation-induced Bystander Effect of Hepatoma Cells[J]. International Journalof Radiation Biology, 2015,24(2):1.
[38] Jiang Y, Chen X, Tian W. The Role of TGF-β1-miR-21-ROS Pathway in Bystander Responses Induced by Irradiated Non-small-cell Lung Cancer Cells[J]. British Journal of Cancer, 2014,111(4):772.
[39] 周海, 潘晓军. 一氧化氮及一氧化氮合酶在急性吸入高浓度氧大鼠肺泡上皮细胞凋亡中的作用[J]. 中国病理生理杂志, 2012,28(1):109.
[40] 邵春林, FOLKARD Melvyn, PRISE K M. 一氧化氮在亚细胞结构精确照射诱导旁效应中的作用[J]. 辐射研究与辐射工艺学报, 2007,25(2):120.
[41] 蔡宇伽, 张红. X射线诱导的HeLa细胞旁效应中ROS和NO关系研究[J]. 原子核物理评论, 2009,26(30):254.
[42] 李青, 徐树红. 神经肽P物质对高氧肺损伤的保护作用调控机制研究[J].重庆医学, 2014,43(21):2749.
[43] 傅应亚, 黎友伦. JNK信号通路与细胞凋亡[J]. 重庆医科大学学报, 2014,39(3):281.
[44] Zhou H N, Ivanov V N, Gillespie J G. Mechanism of Radiation-induced Bystander Effect Role of the Cyclooxygenase-2 Signaling Pathway[J]. Proceedings of the National Academy of Sciences of the United States of America, 2005,102(41):14641.
[45] Dong C, He M, Ren R, et al. Role of the MAPK Pathway in the Observed Bystander Effect in Lymphocytes Co-cultured with Macrophages Irradiated withγ-Rays or Carbon Ions[J]. Life Science, 2015,3205(15):134.