关键词: 相对端粒长度, 人淋巴细胞, ¹²C离子, 定量PCR

Abstract: This paper is to investigate the effect of¹²C ion irradiation on telomere length in culture human lymphocytes. Culture human lymphocyte line Peng-EBV were exposed to ¹²C at 0.3-0.5 Gy/min at doses of 0,0.1,0.5,1.0 and 2.0 Gy, respectively. The cells were collected at 24, 48, 72 hours after exposure, respectively. Genome DNA was extracted. Relative telomere length was measured by fluorescence quantitative PCR. The relative telomere length obviously increased at 48 and 72 hours in each dose group for exposed cells. ¹²C ion irradiation may all result in telomere extension in culture human lymphocytes.

Key words: Telomere length, Human lymphocytes, ¹²C ion, Fluorescence quantitative PCR