代谢组学及其在辐射防护研究中的应用研究进展

摘要/Abstract

摘要： 代谢组学是重要的生物学技术,该技术在辐射防护领域中的应用日益广泛,其中很多研究内容立足于寻找代谢产物中与辐射剂量相关的生物标志物。本文介绍了代谢组学分析检测技术和研究方法特点,简述代谢组学在辐射防护研究中有关动物和人的生物标志物方面的研究进展。

关键词: 代谢组学, 辐射防护, 生物标志物

Abstract: Metabolomics is an important biological technology. This technology is increasingly and widely applied in the field ofradiation protection,with many focusing on identifying the radiation dose-related biomarkers in metabolites. This paper introduces the concept, metabonomics,research methods and features, as well as the progress in biomarker research ofanimal and human in the radiation protection area.

Key words: Metabonomics, Radiation protection, Biomarker

中图分类号:

Q691,TL818

陈忠民, 南新中, 刘波, 刘亮. 代谢组学及其在辐射防护研究中的应用研究进展[J]. 辐射防护通讯, 2014, 34(2): 28-31.

Chen Zhongmin, Nan Xinzhong, Liu Bo, Liu Liang. Advance of Metabonomics and its Application in Radiation Protection[J]. RADIATION PROTECTION BULLETIN, 2014, 34(2): 28-31.

参考文献

[1] Nicholson J K, Lindon J C, Holmes E. ‘Metabonomics’: Understanding the Metabolic Responses of Living Systems to Pathophysiological Stimuli via Multivariate Statistical Analysis of Bbiological NMR Spectroscopic Data[J]. Xenobiotica, 1999,29(11):1181.
[2] Monton M R N, Soga T. Metabolome Analysis by Capillary Electrophoresis-Mass Spectrometry[J]. Journal of Chromatography A, 2007,1168:237.
[3] Bill L. Metabolomics: What's Happening Downstream of DNA[J]. Environ Health Perspect, 2004,112(7):410.
[4] Suhre K, Meisinger C, Döring A, et al. Metabolic Footprintof Diabetes: a Multiplatform Metabolomics Study in an Epidemiological setting[J]. Public Library of Science One, 2010,5(11):13953.
[5] Jung J Y, Kim I Y, Kim Y N, et al. ¹H NMR-based Metabolite Profiling of Diet-Induced Obesity in a Mouse Mode[J]. BMB Reports, 2012,45(7):419.
[6] Zhao Y, Fu L, Li R, et al. Metabolic Profiles Characterizing Different Phenotypes of Polycystic Ovary Syndrome: Plasma Metabolomics Analysis[J]. BMC Medicine, 2012,10(1):153.
[7] Zhang Y, Yang C, Wang S, et al. Liver Atlas: a Unique Integrated Knowledge Database for Systems-Level Research of Liver and Hepatic Disease[J]. Liver International, 2013,33(8):1239.
[8] Bain J R, Stevens R D, Wenner B R, et al. Metabolomics Applied to Diabetes Research: Moving from Information to Knowledge[J]. Diabetes, 2009,58(11):2429.
[9] 许国旺, 杨军. 代谢组学及其研究进展[J]. 色谱, 2003,21(4):310.
[10] Tyburski J B, Patterson A D, Krausz K W, et a1. Radiation Metabolomics: Identification of Minimally Invasive Urine Biomarkers for Gamma-radiationExposure in Mice[J]. Radiation Research, 2008,170(1):1.
[11] Khan A R, Rana P, Devi M M, et al. Nuclear Magnetic Resonance Spectroscopy-based Metabonomic Investigation of Biochemical Effects in Serum of Irradiated Mice[J]. International Journal of Radiation Biology, 2011,87(1):91.
[12] Lanz C, Patterson A D, Slavik J, et a1. Radiation Metabolomics Biomarker Discovery in the Urine of Gamma-Irradiated Rats Using a Simplified Metabolomics Protocol of Gas Chromat Graphy-Mass Spectrometry Combined withRandom Forests Machine Learningalgorithm[J]. Radiation Research, 2009,172(2):198.
[13] Johnson C H, Patterson A D, Krausz K W, et al. Radiation Metabolomics.4.UPLC-ESI-QTOFMS-Based Metabolomics for Urinary Biomarker Discovery in Gamma-IrradiatedRats[J]. Radiation Research, 2011,175(4):473.
[14] Coy S L, Cheema A K, Tyburski J B, et al. Radiation Metabolomics and its Potential in Biodosimetry[J]. International Journal of Radiation Biology, 2011,87(8):802.
[15] Liu H X, Wang Z D, Zhang X Q, et al. Selection of Candidate Rradiation Biomarkers in the Serum of Rats Exposed to Gama-rays by GC/TOFMS-based Metabolomics[J]. Radiation Protection Dosimetry, 2013,154(1):9.
[16] 张慧芳, 党旭红, 邢利虹, 等. 大鼠尿液中γ辐射损伤特征代谢物[J].辐射防护通讯, 2013,33(4):8.
[17] 李艳丽, 王洪彬, 郭灿雄, 等. 利用基于¹H-NMR的代谢组学分析研究重离子辐射对大鼠额叶皮质区的影响[J]. 波谱波学杂志, 2010,27(1):103.
[18] Johnson C H, Patterson A D, Krausz K W, et al. Identification of Urinary Biomarkers of Ionizing Radiation Exposure in Nonhuman Primates by Mass Spectrometry-Based Metabolomics[J]. Radiation Research, 2012,178(4):328.
[19] Hu Z P, Kim Y M, Sowa M B, et al. Metabolomic Response of Human Skin Tissue to Low Dose Ionizing Radiation[J]. Molecular BioSystems, 2012,8(7):1979.