神经肽P物质对放射损伤皮肤成纤维细胞周期及凋亡的影响

Abstract

Abstract: To explore the effects of substance P on cell cycle and apoptosis of irradiated skin fibroblasts, Human Foreskin Fibroblasts (HFF-1) in logarithmic growth phase were divided into five experimental groups: 0 Gy group, 12 Gy group, 18 Gy group, 12 Gy+SP group and 18 Gy+SP group. The experimental groups were irradiated with electron beam at doses of 12 Gy and 18 Gy, while the 12 Gy+SP group and 18 Gy+SP group were treated with 10^-7 mol/L SP one hour before irradiation. After 48 hours of irradiation, cell cycle and apoptosis were detected by flow cytometry. The expression of apoptosis-related genes Bcl-2 and Bax in cells of each experimental group was quantitatively detected by real-time quantitative PCR. Cell cycle detection showed that the percentage of cells in G₂ phase in 12 Gy and 18 Gy experimental groups was significantly higher than that in 0 Gy group. And, compared with the 18 Gy experimental group, the percentage of cells in G₂ phase in the 18 Gy+SP experimental group was significantly lower than that in the 18 Gy group. Apoptosis detection showed that compared with 0 Gy experimental group, the apoptosis rate of 12 Gy and 18 Gy irradiation group increased significantly, while the apoptosis rate of substance P intervention group (12 Gy+SP, 18 Gy+SP) was significantly lower than that of radiation group (12 Gy, 18 Gy). Real-time fluorescence quantitative PCR detection of Bcl-2 and Bax expression showed that compared with 0 Gy experimental group, Bax gene expression was significantly increased and Bcl-2 gene expression was significantly decreased in 12 Gy and 18 Gy irradiated groups. And, the expression of Bcl-2 gene in substance P intervention group (12 Gy+SP and 18 Gy+SP) was significantly higher than that in radiation group (12 Gy and 18 Gy), but there was no significant difference in Bax gene expression. The above results suggest that electron irradiation can induce apoptosis and cell G₂ phase arrest of human skin fibroblasts. Substance P can inhibit the apoptosis and G₂ phase arrest of human skin fibroblasts damaged by radiation.

Key words: substance P, human skin fibroblasts, radiation injury, cell apoptosis, cell cycle

CLC Number:

LIU Xiaoming, DANG Xuhong, ZHANG Ruifeng, LI Xiaozhen, YUAN Yayi, LIU Hongyan, CHAI Dongliang, REN Yue, ZHANG Zhongxin, ZUO Yahui. Effects of substance P on cell cycle and apoptosis of irradiated skin fibroblasts[J].RADIATION PROTECTION, 2023, 43(1): 83-89.

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Effects of substance P on cell cycle and apoptosis of irradiated skin fibroblasts

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