黄芪甲苷对肝细胞放射损伤预防作用的机制研究

Abstract

Abstract: The radioprotection effects and molecular mechanism of Astragaloside IV (ASIV) on the liver cell L-02 was studied. The experiments were conducted with four groups: the control group, the ASIV group, the irradiation group and the ASIV+irradiation group. The rate of apoptosis and cell cycle were detected by flow cytometry and Rhodamine-123 fluorescent probe was used to detect potential alternation of the mitochondrial membrane in L-02 cell. γH2AX foci were detected by immunofluorescence confocal microscopy while the expressions of Nrf2, HO-2, NQO1 proteins were observed by western blot method. The results showed that the apoptosis rate and the arrested cell cycle G2 decreased with increasing concentrations of Astragaloside IV after irradiation and the membrane potential increased with the rise of Astragaloside IV concentration as observed by spectrophotofluorimetry after Rh123 dyeing. The confocal microscopy results showed that the number of γH2AX focus in Astragaloside+irradiation group was significantly less than in the irradiation group. Western Blot results indicated that the expression of Nrf2, HO 1-and NQO1 increased in the irradiated cells, while the opposite trend showed in Astragaloside + irradiation group with increasing drug concentration.

Key words: Astragaloside IV, apoptosis, cell cycle, γH2AX, Nrf2

CLC Number:

Q691

Hu Yameng, Long Ying, Chen Kelian, Liu Bengbo, He Shuya, Huang Bo. Mechanism of radioprotection effects of Astragaloside IV on the liver cell[J].RADIATION PROTECTION, 2017, 37(4): 309-316.

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Mechanism of radioprotection effects of Astragaloside IV on the liver cell

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