盐诱导激酶2对电离辐射诱导的自噬与凋亡的影响

Abstract

Abstract: The purpose of this study is to explore the effects of salt-inducible kinase 2 (SIK2) on autophagy and apoptosis induced by ionizing radiation in the hope of providing a new idea for tumor radiotherapy. Lentivirus LV-GFP and LV-SIK2 were transfected into HeLa cells in vitro. The success of transfection was examined by fluorescence microscopy after 48 h. Real-time PCR and Western blot were used to measure the efficiency of the SIK2 knockdown. After exposure to 8 Gy ⁶⁰Co γ irradiation, the effects of the SIK2 knockdown on autophagy related protein LC3-II and on the P62 expression were examined by Western blot to observe the changes of autophagy. Flow cytometry was performed to examine the effect of the SIK2 knockdown on the apoptosis after 8 Gy ⁶⁰Co γ irradiation. The results showed that the SIK2 knockdown of stably transfected cell line was established successfully. In the down-regulating SIK2 cell line after 8 Gy γ irradiation, the expression of LC3-II was down-regulated while the P62 expression was up-regulated, indicating that autophagy was reduced. Flow cytometric analysis showed that FITC⁺/PI^- cells ratio was already increased significantly at 12 h post irradiation in the down-regulating SIK2 cell line, indicating that the SIK2 knockdown increased the cell apoptosis. In conclusion, the SIK2 knockdown could lead to a significant increase of cell apoptosis induced by ionizing radiation.

Key words: SIK2, ionizing radiation, autophagy, apoptosis, tumor radiotherapy

CLC Number:

Q691

Li Chao, Li Zhongqiu, Li Xueping, Yang Yang, Zeng Yan, Pan Xiujie, Yang Zhihua, Zhu Maoxiang, Gu Yongqing. Effects of salt-inducible kinase 2 on autophagy and apoptosis induced by ionizing radiation[J].RADIATION PROTECTION, 2017, 37(3): 214-222.

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Effects of salt-inducible kinase 2 on autophagy and apoptosis induced by ionizing radiation

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