四君子汤对人小肠上皮细胞辐射损伤的保护作用

Abstract

Abstract: Irradiation-induced intestinal injury is a common complication caused by radiotherapy of abdominal and pelvic malignant tumors, which seriously affects the quality of patients’ life. It has been reported that Sijunzi Decoction (Four Noble Drugs Decoction) showed a protective effect on BALB/c mice after irradiation. To further explore the role of Sijunzi Decoction (Four Noble Drugs Decoction) in intestinal radiation damage, in this paper, human intestinal epithelial cells(HIEC-6)was used as the subject to investigate the protective effects of Sijunzi Decoction (Four Noble Drugs Decoction) on radiation damage. The experimental results showed that 0.95 mg/mL Sijunzi Decoction (Four Noble Drugs Decoction) could repair radiation damage on HIEC-6, reduce apoptosis, and slow down cell cycle block. The mechanism of Sijunzi Decoction (Four Noble Drugs Decoction) reducing radiation damage was related to the regulation on protein expression of Bcl-2 and γ H2AX. This work provided a reference for the treatment of radiation-induced intestinal injury by using Sijunzi Decoction (Four Noble Drugs Decoction).

Key words: Sijunzi Decoction (Four Noble Drugs Decoction), ionizing radiation, radiation protection, human intestinal epithelial cells

CLC Number:

R142+.4

WANG Chengfang, SHAO Shuai, QU Gonglin, QI Xuesong, GOU Qiao. The protection effect of Sijunzi Decoction (Four Noble Drugs Decoction) on radiation damage of human intestinal epithelial cells[J].RADIATION PROTECTION, 2020, 40(5): 456-461.

References

[1] ZHANG H,YAN H,ZHOU X,et al.The protective effects of Resveratrol against radiation-induced intestinal injury [J]. BMC Complement Altern Med, 2017, 17(1):410.
[2] 姚斌伟,彭瑞云,高亚兵,等. 安多霖对微波辐射致大鼠生殖远后损伤的保护作用研究 [J]. 中国体视学与图像分析,2014,19(3):272-279.
YAO Binwei, PENG Ruiyun, GAO Yabing, et al.Protective effect of a Chinese medicine composite (Anduolin) on the long-term reproductive damage in rats exposed to microwave radiation [J]. Chinese Journal of Stereology and Image Analysis, 2014,19(3):272-279.
[3] XU P, CAI X, ZHANG W, et al. Flavonoids of Rosa roxburghii Tratt exhibit radioprotection and anti-apoptosis properties via the Bcl-2 (Ca²⁺)/Caspase-3/PARP-1 pathway [J]. Apoptosis, 2016, 21(10):1125-1143.
[4] 段雅彬, 姚星辰, 张娟玲, 等. 中药抗辐射作用研究进展[J]. 药学研究,2015,34(2):95-99.
DUAN Yabin, YAO Xingchen, ZHANG Juanling, et al. Research advance on anti-radiation effect of traditional Chinese medicine [J]. Journal of Pharmaceutical Research,2015,34(2):95-99.
[5] 李辰,邵帅,曲功霖,等. 四君子汤对受照小鼠体重及外周血象的影响[J]. 辐射防护,2015, 35(6):345-350.
LI Chen, SHAO Shuai, QU Gonglin, et al. Effects of Sijunzi decoction on body weight and peripheral blood in ⁶⁰Co γ irradiated mice [J]. Radiation Protection,2015, 35(6):345-350.
[6] 邵帅,曲功霖,田梅,等. 四君子汤对电离辐射所致小鼠免疫损伤和骨髓损伤的防治作用[J]. 中华放射医学与防护杂志,2015, 35(11)::815-818.
SHAO Shuai, QU Gonglin, TIAN Mei, et al. Protective effect of Sijunzi Tang on mouse immune damage and bone marrow damage caused by ionizing radiation [J]. Chinese Journal of Radiological Medicine and Protection,2015, 35(11):815-818.
[7] 曲功霖, 邵帅, 李辰,等. 四君子汤抗辐射活性部位的初步筛选[J]. 辐射防护,2017,37(4):303-308.
QU Gonglin, SHAO Shuai, LI Chen, et al. Screening of radiation protective constituents of Sijunzi Decoction [J]. Radiation Protection,2017,37(4):303-308.
[8] Ishihara H,Tanaka I,Yakumaru H,et al.Pharmaceutical drugs supporting regeneration of small intestinal mucosa severely damaged by ionizing radiation in mice [J].J Radiat Res,2013,54(6):1057-1064.
[9] Matsuzaki-Horibuchi S,Yasuda T,Sakaguchi N,et al.Cell-permeable intrinsic cellular inhibitors of apoptosis protect and rescue intestinal epithelial cells from radiation-induced cell death [J].J Radiat Res, 2015, 56(1):100-113.
[10] Gurley K E,Ashley A K,Moser R D,et al.Synergy between Prkdc and Trp53 regulates stem cell proliferation and GI-ARS after irradiation[J].Cell Death Differ, 2017, 24(11):1853-1860.
[11] 代金明,林汝仙,王升启. 细胞周期调控与细胞抗辐射能力[J]. 辐射研究与辐射工艺学报,2005,23(5):262-265.
DAI Jinming, LIN Ruxian, WANG Shengqi. Cell cycle modulation and radiation resistance [J]. Journal of Radiation Research and Radiation Processing,2005,23(5):262-265.
[12] 崔钰珠, 于慧会, 崔巧妍, 等. 四君子汤加减改善大肠癌患者生存质量研究进展[J]. 辽宁中医药大学学报,2017,19(2):74-76.
CUI Yuzhu, YU Huihui, CUI Qiaoyan,et al.Research of Sijunzi Decoction on improving life quality of colorectal cancer patients [J]. Journal of Liaoning University of Traditional Chinese Medicine,2017,19(2):74-76.
[13] 张广唱, 武哲丽, 李杰斌. 四君子汤治疗肝癌的临床研究进展[J]. 辽宁中医杂志,2015,42(1):218-220.
ZHANG Guangchang, WU Zheli, LI Jiebin. Clinical research progress of Sijunzi Decoction in treatment of hepatic carcinoma [J]. Liaoning Journal of Traditional Chinese Medicine,2015,42(1):218-220.

The protection effect of Sijunzi Decoction (Four Noble Drugs Decoction) on radiation damage of human intestinal epithelial cells

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