RAN基因沉默对辐射诱发基因组不稳定肝细胞HL-7702凋亡及细胞周期的影响

Abstract

Abstract: RNAi was used to inhibit RAN gene’s transcription and expression level of RAN. The apoptosis was detected by Annex in Ⅴ-APC flow cytometry (FCM) and the cell cycle was determined by FCM with single PI staining. Results of real time fluorescence quantitative PCR tests demonstrated that RAN gene can be inhibited efficiently by RNAi. The apoptosis percentage in RNAi group was lower than that in the negative control, the number of cells in G₁ phase were higher than in the negative control. These results indicated that RAN gene may maintain the genomic stability through regulating the cell apoptosis and cell cycles.

Key words: RAN gene, genomic instability, RNA interference, cell cycle, cell apoptosis

CLC Number:

Dang Xuhong, ZuoYahui, Yuan Yayi, Liu Hongyan, Liu Jiangong, Zhang Ruifeng. Effects of RAN Gene Silencing by siRNA on Apoptosis and Cell Cycle in Radiation Induced Genomic Instable Liver Cells[J].RADIATION PROTECTION, 2014, 34(6): 361-365.

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Effects of RAN Gene Silencing by siRNA on Apoptosis and Cell Cycle in Radiation Induced Genomic Instable Liver Cells

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