2型转谷氨酰胺酶对人胶质母细胞瘤细胞U251放射敏感性的影响

Abstract

Abstract: To investigate the effect of transglutaminase 2 (TG2) on radiosensitivity of glioblastoma, bioinformatic analysis was adopted to analyze the relationship between TG2 expression and the prognosis of glioma patients and TG2 level in GBM radioresistant cell line U251; TG2-overexpression cells (U251-TG2) or control cells (U251-EV) were obtained by transient transfection; cell growth curve was drawn by CCK8 method, and cell migration ability was measured by transwell; colony formation ability, ROS level and γ-H2AX foci were measured after X-ray treatment; Beclin-1, LC3 and mTOR phosphorylation levels were detected by Western blot. The results showed that glioma patients with high TG2 expression had worse progression-free interval and overall survival, and TG2 expression in radioresistant U251 was significantly higher than that in wild type U251; the cell proliferation and migration ability of U251-TG2 were higher than that of U251-EV; after X-ray treatment, compared with U251-EV, U251-TG2 showed higher colony-forming ratio, lower ROS level and γ-H2AX foci number; the levels of Beclin-1 and LC3-II/I in U251-TG2 were higher than those in U251-EV; mTOR phosphorylation level in U251-TG2 was lower than that in U251-EV. The results indicated that TG2 could promote autophagy by inhibiting mTOR phosphorylation, and then decrease the radiosensitivity of U251 cells.

Key words: glioblastoma, transglutaminase 2, radiosensitivity

CLC Number:

Q274

LAN Ruilong, WU Bing, WANG Zeng. Effects of transglutaminase 2 on radiosensitivity of human glioblastoma cell line U251[J].RADIATION PROTECTION, 2024, 44(5): 537-545.

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Effects of transglutaminase 2 on radiosensitivity of human glioblastoma cell line U251

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