双着丝粒染色体半自动化分析估算辐射生物剂量专家共识

Abstract

Abstract: Over the past ten years, semi-automatic analysis of dicentric chromosome (dic) has been widely used for estimating radiation biological dose internationally due to its technical maturity. However, there are still no relevant technical specifications and standards in China, although both technical reports published by the IAEA and technical standards published by the International Organization for Standardization have recommended this method for dose estimation. The present technical consensus of biological dose estimation was established from the aspects of the dic semi-automatic analysis principle, main technical content, factor analysis, and application examples, based on more than 30 years of practical experience for dic manual and semi-automatic analysis at home and abroad. Compared with the existing national standard GB/T 28236, it could significantly improve the efficiency of biological dose estimation, and could also reduce the requirements for technical proficiency of professionals, thus could be better promoted and applied. Similar to manual analysis, dic semi-automatic analysis could be used to estimate and reconstruct the exposed dose under different radiation exposure scenarios including acute uniform, local, extensional irradiation, and delayed sampling. Considering the inefficiency of current domestic dose estimation which cannot meet the needs of clinical classification and diagnosis for large-scale nuclear and radiation accidents that have a large number of exposed people, the promotion and application of dic semi-automatic analysis might solve these bottleneck problems and provide technical support for further development of relevant national standard.

Key words: ionizing radiation, dicentric chromosome, semi-automatic analysis, biodosimetry, expert consensus

CLC Number:

R144.1

Nuclear Emergency Medicine Branch of Chinese Nuclear Society, Chinese Society of Radiological Medicine and Protection of Chinese Medical Association, Radiological Health Professional Committee of Chinese Preventive Medicine Association, Nuclear and Radiation Emergency Response Branch of China Society of Radiation Protection. Expert consensus on radiation biodosimetry by semi-automatic analysis of dicentric chromosome[J].RADIATION PROTECTION, 2024, 44(3): 199-209.

References

[1] HAN L, GAO Y, WANG P, et al. Cytogenetic biodosimetry for radiation accidents in China [J]. Radiat Med Prot, 2020, 1(3): 133-139.
[2] 王平, 韩林, 李杰, 等. 双着丝粒染色体分析在核与辐射事故应急生物剂量估算中的应用与进展[J]. 辐射防护通讯, 2020, 40(Z1): 97-102.
WANG P,HAN L, LI J, et al. Application of dicentric chromosome analysis in nuclear/radiation emergency and its progress [J]. Radiat Prot Bulletin, 2020, 40(Z1): 97-102.
[3] International Atomic Energy Agency. Cytogenetic dosimetry: Application in preparedness for and response to radiation emergencies[R].Vienna: IAEA, 2011.
[4] International Organization for Standardization. Radiation protection—Performance criteria for laboratories performing initial cytogenetic dose assessment of mass casualties in radiological or nuclear emergencies—General principles and application to dicentric assay:ISO 21243[S]. ISO, Geneva, 2022.
[5] 中华人民共和国卫生部中国国家标准化管理委员会. 染色体畸变估算生物剂量方法:GB/T 28236—2011 [S]. 北京:中国标准出版社, 2012.
[6] Romm H, Ainsbury E, Barnard S, et al. Automatic scoring of dicentric chromosomes as a tool in large scale radiation accidents [J]. Mutat Res, 2013, 756(1-2):174-183.
[7] 戴宏, 刘玉龙, 冯骏超, 等. 双着丝粒染色体自动分析生物剂量估算研究[J]. 中华放射医学与防护杂志, 2017, 37(3):182-186.
[8] 中华人民共和国国家卫生和计划生育委员会. 放射工作人员职业健康检查外周血淋巴细胞染色体畸变检测与评价:GBZ 248—2014 [S].北京:中国标准出版社, 2014.
[9] 陈英, 骆亿生, 曹珍山, 等. 特大剂量γ射线照射的染色体畸变剂量效应曲线[J]. 辐射研究与辐射工艺学报, 2006, 24(4): 241-244.
[10] 陈英, 杜杰, 张学清, 等. 太原“4.11”钴源事故受照者生物剂量估算及照后一年细胞遗传学随访[J]. 辐射防护, 2010, 30(4): 201-207.
CHEN Y, DU J, ZHANG X Q, et al. Biological dose estimation in victims of Taiyuan “4.11” cobalt source accident and cytogenetic follow-up observation after one year [J]. Radiat Prot, 2010, 30(4): 201-207.
[11] Deperas J, Szluinska M, Deperas-Kaminska M, et al. CABAS: a freely available PC program for fitting calibration curves in chromosome aberration dosimetry[J]. Radiat Prot Dosim, 2007, 124(2): 115-123.
[12] Endesfelder D, Oestreicher U, Bucher M, et al. RENEB Inter-Laboratory Comparison 2021: The Dicentric Chromosome Assay[J]. Radiat Res, 2023, 199(6):556-570.
[13] Vaurijoux A, Gruel G, Pouzoulet F, et al. Strategy for population triage based on dicentric analysis[J]. Radiat Res, 2009, 171(5): 541-548.
[14] 韩林, 陆雪, 李杰, 等. 双着丝粒体半自动与人工分析估算生物剂量的比较研究[J].中华放射医学与防护杂志, 2020, 40(11): 826-831.
HAN L, LU X, LI J, et al. Study on the comparison of semi-automatic and manual dicentric detection for biological dosimetry [J]. Chin J Radiol Med Prot, 2020, 40(11): 826-831.
[15] Gruel G, Gregoire E, Lecas S, et al. Biological Dosimetry by automated dicentric scoring in a simulated emergency [J]. Radiat Res, 2013, 179(5): 557-569.
[16] Ryan T L, Escalona M B, Smith T L, et al. Optimization and validation of automated dicentric chromosome analysis for radiological/nuclear triage application [J]. Mutat Res, 2019, 847: 503087.
[17] Romm H, Ainsbury E, Barnard S, et al. Validation of semi-automatic scoring of dicentric chromosomes after simulation of three different irradiation scenarios [J]. Health Phys, 2014, 106(6): 764-771.
[18] 韩林, 张冰洁, 王平, 等. 一起介入治疗意外照射导致背部大面积放射性皮肤损伤患者的生物剂量估算[J]. 中华放射医学与防护杂志, 2021, 41(12):886-891.
HAN L, ZHANG B J, WANG P, et al. Biodosimetry estimation of a case of large area back skin injury caused by accidental irradiation in interventional procedure [J]. Chin J Radiol Med Prot, 2021, 41(12):886-891.
[19] 吕玉民, 刘玉龙, 沈军生, 等. 大规模核与辐射事故受照人员生物剂量估算的应对策略[J]. 辐射防护通讯, 2022, 42(4-5): 69-73.
LYU Y M, LIU Y L, SHEN J S, et al. Strategy of biological dosimetry for those exposed to large-scale nuclear/radiological incidents [J]. Radiat Prot Bulletin, 2022, 42(4-5): 69-73.

Expert consensus on radiation biodosimetry by semi-automatic analysis of dicentric chromosome

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 10

[1]	HU Weixiang, ZHAO Hongling, ZHANG Yu, MA Lanfang, ZHOU Pingkun, GUAN Hua. Study on the protective effect of hesperidin and hesperetin on radiation-induced cardiovascular injury in mice [J]. RADIATION PROTECTION, 2024, 44(3): 304-314.
[2]	WANG Zhiyun, GOU Wenfeng, GUO Jianghong, XU Feifei, LI Yiliang, HOU Wenbin. Study on the protective effect of Ampelopsis grossedentata extract on the hematopoietic system damage caused by ionizing radiation [J]. RADIATION PROTECTION, 2024, 44(2): 182-191.
[3]	Nuclear Emergency Medicine Branch of the Chinese Nuclear Society; Chinese Society of Radiological Medicine and Protection, Chinese Medical Association; Radiological Health Professional Committee of Chinese Preventive Medicine Association; Nuclear and Radiation Emergency Response Branch of the Chinese Society of Radiation Protection. Expert consensus on occupational health surveillance of radiation workers [J]. RADIATION PROTECTION, 2024, 44(2): 101-109.
[4]	Nuclear and Radiation Emergency Medicine Branch of the Chinese Nuclear Society, Nuclear and Radiation Emergency Branch of China Society of Radiation Protection Safety, Radiological Health Professional Committee of Chinese Preventive Medicine Association, Environmental Protection and Waste Management Committee of Science and Technology Commission of China National Nuclear Corporation. Expert consensus on clinical diagnosis and treatment techniques for local radiation injuries caused by external Iridium-192 source irradiation [J]. RADIATION PROTECTION, 2023, 43(5): 393-411.
[5]	WANG Yangyang, LIU Yuanduo, LIU Lian. Effects of ionizing radiation on the development of cerebellum and prefrontal cortex in mice during neonatal period [J]. RADIATION PROTECTION, 2023, 43(3): 271-279.
[6]	WANG Ping, FAN Li, LU Xue, GAO Ling, LIU Qingjie, TIAN Mei. Identification of differential mRNA expression profiles in lens epithelial cells induced by low-dose ionizing radiation [J]. RADIATION PROTECTION, 2023, 43(2): 175-185.
[7]	DAI Yongzhi, XIA Houqiong, CAO Jinjia, XIAO Hailiang, CHEN Qiang, LI Fang. The effect of ⁶⁰Co γ irradiation on the electrostatic adsorption efficiency of protection masks [J]. RADIATION PROTECTION, 2022, 42(3): 229-235.
[8]	HUANG Yue, CHEN Naiyao, ZHAO Hui, YAN Zhenyu, ZHANG Haixia, ZHAO Xuecong, ZHANG Dingping. Effects of N-acetylcysteine on oxidative stress, proliferation and apoptosis of HT22 cells induced by radiation [J]. RADIATION PROTECTION, 2021, 41(2): 165-173.
[9]	WANG Chengfang, SHAO Shuai, QU Gonglin, QI Xuesong, GOU Qiao. The protection effect of Sijunzi Decoction (Four Noble Drugs Decoction) on radiation damage of human intestinal epithelial cells [J]. RADIATION PROTECTION, 2020, 40(5): 456-461.
[10]	GAO Fei, CHEN Dongfeng, XIAO Xuefu, XU Yang. Study on pulsed X ray response characteristics of active ionization radiation dosimeters [J]. RADIATION PROTECTION, 2019, 39(6): 469-474.
[11]	DANG Xuhong, ZUO Yahui, WANG Fang, ZHANG Jingyun, DONG Juancong, ZHANG Zhongxin, DUAN Zhikai. Detection and analysis of MXR7 gene expression in human liver cells induced by radiation and peripheral blood of radiation workers [J]. RADIATION PROTECTION, 2018, 38(6): 517-521.
[12]	Li Chao, Li Zhongqiu, Li Xueping, Yang Yang, Zeng Yan, Pan Xiujie, Yang Zhihua, Zhu Maoxiang, Gu Yongqing. Effects of salt-inducible kinase 2 on autophagy and apoptosis induced by ionizing radiation [J]. RADIATION PROTECTION, 2017, 37(3): 214-222.
[13]	Zheng Junzheng. New progress in the field of radiological protection [J]. RADIATION PROTECTION, 2016, 36(6): 393-407.
[14]	Zheng Junzheng. Overview on safety standards of International Atomic Energy Agency [J]. RADIATION PROTECTION, 2016, 36(4): 252-259.
[15]	Wang Bin, Zhang Ying, Zhao Degen, Li Zhongqiu, Yang Yang, Li Chao, Zhu Maoxiang, Gu Yongqing. Changes of PIF1 gene expression induced by ⁶⁰Co γ ray in HepG2 [J]. RADIATION PROTECTION, 2016, 36(3): 149-153.