端粒长度检测及其在辐射损伤研究中的应用

辐射防护通讯 ›› 2016, Vol. 36 ›› Issue (1): 23-27.

端粒长度检测及其在辐射损伤研究中的应用

郭月凤, 张睿凤

中国辐射防护研究院, 太原, 030006

收稿日期:2015-12-28 出版日期:2016-02-20 发布日期:2021-11-19
作者简介:郭月凤(1962- ),女,1983年毕业于北京大学生物系生理专业,学士; 研究员。主要从事放射医学研究。

Telomere Detection and Its Application in Radiation Damage Study

Guo Yuefeng, Zhang Ruifeng

China Institute for Radiation Protection, Shanxi, Taiyuan, 030006

Received:2015-12-28 Online:2016-02-20 Published:2021-11-19

摘要/Abstract

摘要： 介绍了端粒长度主要检测方法TRF、Q-FISH、FlowFISH、Q-PCR、STELA的基本原理及其优缺点,端粒检测在辐射损伤研究及辐射流行病学调查和临床研究中的应用,以及本实验室改进后的Q-PCR方法及其应用。

关键词: 端粒, 长度变异, 检测, 辐射损伤

Abstract: The principle, forte and weakness of telomere length measurement methods such as TRF, Q-FISH, flow FISH, Q-PCR, STELA are reviewed. The telomere detection means used in radiation damage study, radiation epidemiological survey and clinical study are then introduced, along withthe improved Q-PCR in CIRP and its application.

Key words: Telomere, Length variation, Measurements, Radiation damage

中图分类号:

R811

郭月凤, 张睿凤. 端粒长度检测及其在辐射损伤研究中的应用[J]. 辐射防护通讯, 2016, 36(1): 23-27.

Guo Yuefeng, Zhang Ruifeng. Telomere Detection and Its Application in Radiation Damage Study[J]. RADIATION PROTECTION BULLETIN, 2016, 36(1): 23-27.

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